Liquid chromatography/mass spectrometry capillaries

Liquid Chromatography-Mass Spectrometry Vaporizer Seated capillary Skimmer... 

Figure 5.54 Structures of Praziquantel and its metabolites, cis- and fraw5-4-hydroxy-praziquantel. Reprinted from 7. Chromatogr., B, 708, Lerch, C. and Blaschke, G., Investigation of the stereoselective metabolism of Praziquantel after incubation with rat liver microsomes by capillary electrophoresis and liquid chromatography-mass spectrometry , 267-275, Copyright (1998), with permission from Elsevier Science.

Opiteck, G.J., Jorgenson, J.W., MacNair, J.E., Moseley, M.A., 3rd (1997). Two-dimensional SEC/RPLC coupled to mass spectrometry for the analysis of peptides. Rapid separation and characterization of protein and peptide mixtures using 1.5 microns diameter non-porous silica in packed capillary liquid chromatography/mass spectrometry. Anal. Chem. 69, 2283-2291. 

Sanz-Nebot, V., Benavente, F., Barbosa, J. (2002). Liquid chromatography-mass spectrometry and capillary electrophoresis combined approach for separation and characterization of multicomponent peptide mixtures application to crude products of leuprolide synthesis. J. Chromatogr. A 950, 99-111. 

All gas chromotography/mass spectrometry (GC/MS) analyses of monomers and intermediates were performed on a Finnigan 1020 GC/MS using a 30-m RSL-150 fused silica capillary column. Liquid chromatography/mass spectrometry (LC/NS) was performed on a Finnigan 4500 mass spectrometer using acetonitrile-water eluent and a moving belt LC/MS interface. 

Many of the more established techniques have been validated through collaborative studies which becomes of greater importance as laboratories seek to become accredited via ISO, EN or related systems where the use of official or well validated methods is mandatory. New instrumental techniques are constantly being reported in the literature but it often requires many years before procedures are introduced, validated and then applied within the food industry. Recent techniques that can be included in this category are capillary electrophoresis and liquid chromatography-mass spectrometry (LC-MS). In time procedures based on these techniques will also become accepted as routine methods and are likely to be adopted by some of the official international bodies like the AOAC International, CEN, ISO, etc. 

C.T. da Costa, D. Horton and S.A. Margolis, Analysis of anthocyanins in foods by liquid chromatography, liquid chromatography-mass spectrometry and capillary electrophoresis. J. Chromatogr. 881 (2000) 403 110. 

Mordehai, A., Lim, H. K., and Henion, J. D. (1995). Ion-spray liquid-chromatography mass-spectrometry and capillary electrophoresis mass-spectrometry on a modified benchtop ion-trap mass-spectrometer. In Practical Aspects of Ion-Trap Mass Spectrometry Chemical, Environmental and Biomedical Applications (R. E. March, and J. F. J. Todd, Eds), Vol. 3, pp. 215—237, CRC Press, Boca Raton, FL. 

Jenkins et al. developed a capillary electrophoresis system for the measurement of iohexol as a marker of the glomerular filtration rate (GFR) with a run time of 5.25 min and a coefficient of variation (CV) of 4.3% at 80 mg L" [121]. The GFR, calculated from the plasma clearance, had a reproducibility of 5.47 %. A similar approach (liquid chromatography-mass spectrometry with positive electrospray ionization after enrichment by solid phase extraction) was applied by Putschew et al. for the determination of iodinated contrast agents in treatment plant effluents and surface waters [118]. 

Davis, M. T., Stahl, D. C., and Lee, T. D., Low-flow high-performance liquid-chromatography solvent delivery system designed for tandem capillary liquid-chromatography mass-spectrometry. Journal of the American Society for Mass Spectrometry 6(7), 571-577, 1995. 

R.E. Sawicki, E. and Krost, K./ Estimation of N-nitroso-dimethylamine Levels in Ambient Air by Capillary Gas-Liquid Chromatography/ Mass Spectrometry, Anal. Lett. 1976, 9(6) 579-594. 

Blum, W., Aichholz, R., Ramstein, P., Kuhnol, J., Bruggen, J., O Reilly, T., andFlorsheimer, A. (2001). In vivo metabolism of epothilone B in tumor-bearing nude mice Identification of three new epothilone B metabolites by capillary high-pressure liquid chromatography/ mass spectrometry/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 15 41-49. 

N. Kawasaki, S. Itoh, M. Ohta, and T. Hayakawa, Microanalysis of JV-linked oligosaccharides in a glycoprotein by capillary liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry, Anal. Biochem., 316 (2003) 15-22. 

Ackermann, B. L. Gillespie, T. A. Regg, B. T. Austin, K. E Coutant, J. E. 1996b. Application of packed capillary liquid chromatography/mass spectrometry with electrospray ionization to the study of the human biotransformation of the anti-emetic drug dolasetron. /. Mass Spectrom., 37,681-689. 

As an instrumental approach to conventional electrophoresis, capillary electrophoresis offers the capability of on-line detection, micropreparative operation and automation (6,8,45-47). In addition, the in tandem connection of capillary electrophoresis to other spectroscopy techniques, such as mass spectrometry, provides high information content on many components of the simple or complex peptide under study. For example, it has been possible to separate and characterize various dynorphins by capillary electrophoresis-mass spectrometry (33). Therefore, the combination of CE-mass spectrometry (CE-MS) provides a valuable analytical tool useful for the fast identification and structural characterization of peptides. Recently, it has been demonstrated that the use of atmospheric pressure ionization using Ion Spray Liquid Chromatography/ Mass Spectrometry is well suited for CE/MS (48). This approach to CE/MS provides a very effective and straightforward method which allow the feasibility of obtaining CE/MS data for peptides from actual biological extracts, i.e., analysis of neuropeptides from equine cerebral spinal fluid (33). 

P. Thibault, Ionspray mass spectrometry of marine toxins. III. Analysis of paralytic shellfish poisoning toxins by flow-injection analysis, liquid chromatography/mass spectrometry and capillary electrophoresis/mass spectrometry, Rapid Commun. Mass Spectrom., 6, 14-24 (1992). 

Niemela K, Sjostrom E (1986) Simultaneous identification of aromatic and aliphatic low molecular weight compounds from alkali pulp liquor by capillary gas-liquid chromatography-mass spectrometry Holzforschung 40 361-368... 

Holzl, G. et al. Analysis of biological and synthetic ribonucleic acids by liquid chromatography-mass spectrometry using monolithic capillary columns. Anal. Chem. 2005,77, 673-680. 

J. Abian, A. J. Oosterkamp, and E. Gelpi, Comparison of conventional, narrow-bore and capillary liquid chromatography/mass spectrometry for electrospray ionization mass spectrometry Practical consideration, J. Mass Spectrom. 34 (1999), 244-254. 

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