Preparative high-performance liquid chromatography

J Rivier, R McClintock, R Galyean, H Anderson. Reversed-phase high-performance liquid chromatography preparative purification of synthetic peptides. J Chromatog 288, 303, 1984. 

As a method of research, has been used high-performance liquid chromatography in reversed - phase regime (RP HPLC). The advantage of the present method is the following the additional information about AIST and FAS composition (homologous distribution) simple preparation of samples (dilution of a CS sample of in a mobile phase). 

SynChropak GPC supports were introduced in 1978 as the first commercial columns for high-performance liquid chromatography of proteins. SynChropak GPC columns were based on research developed by Fred Regnier and coworkers in 1976 (1,2). The first columns were only available in 10-yu,m particles with a 100-A pore diameter, but as silica technology advanced, the range of available pore diameters increased and 5-yu,m particle diameters became available. SynChropak GPC and CATSEC occasionally were prepared on larger particles on a custom basis, but generally these products have been intended for analytical applications. 

Despite the difficulties caused by the rapidly expanding literature, the use of chiral stationary phases (CSPs) as the method of choice for analysis or preparation of enantiomers is today well established and has become almost routine. It results from the development of chiral chromatographic methods that more than 1000 chiral stationary phases exemplified by several thousands of enantiomer separations have been described for high-performance liquid chromatography (HPLC). 

Commercially available poiybrominated aromatic ethers have been analyzed by reversed phase high performance liquid chromatography. NMR spectra of material isolated by preparative methods served to identify the observed peaks as congeners of tetrabromo to nonabromo diphenyl ether. A bromination pathway was clearly indicated. 

Lloyd, L Warner, FP, Preparative High-Performance Liquid Chromatography on a Unique High-Speed Macroporous Resin, Journal of Chromatography 512, 365, 1990. 

High performance liquid chromatography (HPLC) has been by far the most important method for separating chlorophylls. Open column chromatography and thin layer chromatography are still used for clean-up procedures to isolate and separate carotenoids and other lipids from chlorophylls and for preparative applications, but both are losing importance for analytical purposes due to their low resolution and have been replaced by more effective techniques like solid phase, supercritical fluid extraction and counter current chromatography. The whole analysis should be as brief as possible, since each additional step is a potential source of epimers and allomers. 

Colin, H., Hilaireau, P., and de Tournemire, J., Dynamic axial compression columns for preparative high performance liquid chromatography, LC-GC, 8, 302, 1990. 

Snyder, L. R., Dolan, J. W., and Cox, G. B., Preparative high-performance liquid chromatography under gradient conditions. III. Craig simulations for heavily overloaded separations, J. Chromatogr., 484, 437, 1989. 

Torres, A. R., Edberg, S.C., and Peterson, E. A., Preparative high-performance liquid chromatography of proteins on an anion exchanger using unfractionated carboxymethyl displacers, /. Chromatogr., 389, 177, 1987. 

Lee, A. L., Liao, A. W., and Horvath, Cs., Tandem separation schemes for preparative high-performance liquid chromatography of proteins,. Chromatogr., 443, 31, 1988. 

Siitfeld, R., Preparative liquid chromatography with analytical separation quality interval injection/displacement reversed-phase high-performance liquid chromatography, ]. Chromatogr., 464, 103, 1989. 

Herraiz, T., Sample preparation and reversed phase-high performance liquid chromatography analysis of food-derived peptides, Analytica Chimica Acta, 352, 119, 1997. 

Cox, G. B., Loscombe, C. R., Slucutt, M. J., Sugden, K., and Upfield, J. A., The preparation, properties and some applications of bonded ion-exchange packings based on microparticulate silica gel for high-performance liquid chromatography, J. Chromatogr., 117, 269, 1976. 

Suatoni, J.C. Swab, R.E. Preparative Hydrocarbon Compound Type Analysis by High Performance Liquid Chromatography, J. Chromatogr. Sci. 1976,14, 535. 

Threadgill, G.J., Conrad, R.C., Cannon, M., Craven, G.R. (1987). A rapid and preparative method for the separation of yeast ribosomal proteins by using high-performance liquid chromatography. Biochem. J. 244, 523-532. 

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