L929 cells growth

Evaluation of the cell growth by the growth inhibition test according to ISO 10993-5 using mouse fibroblasts L929 and crystal violet as staining substance... 

The title substituted 6 phenylpurine bases and nucleosides 10—13, as well as some acetyl derivatives 8, were tested on their in vitro inhibition of the cell growth in the following cell cultures mouse leukemia L1210 cells (ATCC CCL 219) murine L929 cells (ATCC CCL 1) human cervix carcinoma HeLa S3 cells (ATCC CCL 2.2) and human T lymphoblastoid CCRF-CEM cell line (ATCC CCL 119). Only substituted 6-phenylpurine ribonucleosides 12 and their triacetates 8 exhibited significant activity in these assays (Table 1), while the bases 10 and 11, as well as the 2 amino 6 phenylpurine ribonucleosides 13, were entirely inactive. 

Fig. 4.2. Growth cycle of mouse L929 cells. Mouse L929 cells were inoculated into 2 oz medical flat bottles (5 X 10s cells in 5 ml Eagle s Minimal Essential Medium containing 10% calf serum). The medium was changed every two days. Bottles were incubated for 60 min with 2 /xCi (6-3H)-thymidine (80 /iCi/pmol) at a final concentration of 5 gM after which they were harvested by trypsinisation, their...

Fig. 11.8. Inhibition of cell growth by thymidine. About 10s cells in 5 ml Eagle s MEM, supplemented with extra vitamins and calf serum, were inoculated into 5 cm dishes and incubated in the presence of the indicated concentrations of thymidine. After 4 days the cells were trypsinised and counted using a Coulter counter. , L929 o, HeLa a, BSCI X, X CHO.

A second index of biocompatibility was the quantitative analysis of cell growth inhibition, again on mouse fibroblast L929 cells, induced by the liquid components of the adhesive system and water extracts of two solid crosslinked materials. Table IV is a summary of the percent inhibition of cell growth (percent ICG). The mean protein values at 4 °C have been subtracted from the mean protein values (five test samples) at 37 °C for each treatment condition. The percent inhibition of cell growth (percent ICG) is shown for each treatment condition. The precision of the assay is approximately 10%. 

The data given In Tables 5 and 6 are offered to Illustrate the variety of results. Thus for Poliovirus, platinum polyamines can effect an Increase or decrease In the growth of the virus or exhibit no effect at concentrations below that where the HeLa cells are affected. Similar results are found for the polyamines In L929 cells Infected with EMC strain MM. Thus with treatment for 24 hours, polymers 9,11 and 12 suppressed viral replication (Table 6). With the exception of polymer 6, all of the polymers suppressed EMC viral replication when treatment was extended to 48 hours. Again viral replication was affected at polyamlne concentrations well below that necessary to Inhibit the L929 cells. 

For comparison, the GI for cisplatin for L929 cells is 50pg/ml. Thus, all of the tested compounds except for the tetrachloroplatinate exhibit GI values less than that of cisplatin, and with the exception to the tetrachloroplatinate exhibit similar abilities to inhibit cell growth. 

FIGURE 2.3.9 The growth of L929 cells proliferated inside the 10 and 20 wt% 8-arms PEG-PLLAoptical microscope image of the cross-section of the gel after 7 days. Reprinted with permission from Ref. [84]. 

PHBHHx Hyaluronan coating Improved hydrophilicity, low fibroblast L929 cellular growth 1.6x10 (4x10 ) cells per ml. Could be employed in biomaterial selection and design. 77... 

The biocompatibiUty of PHBHHx were evaluated in vitro The mouse fibroblast cell line L929 was inoculated on films made of PHB, PHBHHx and their blends, polylactic acid (PLA) as control. It was found that the growth of the eells L929 was poor on PHB and PLA films. The viable cell number ranged from 8.8 x 10 to 1.8 x 10" /em only. Cell growth on the films made by blending PHB and PHBHHx showed a dramatie improvement. The viable cell number observed increased from 9.7 x 10 to 1.9 x 10 on a series of PHB/... 

The cytotoxicity test described in ISO 10993-5 is a good example. It is a rapid, standardised test, very sensitive and can characterise materials and significant quantities of harmful extractables and their effect on cellular growth. Because of the high sensitivity, mouse fibroblasts L929 are used as the test cells routinely. 

Chang, N.S., Transforming growth factor-betal blocks the enhancement of tumor necrosis factor cytotoxicity by hyaluronidase Hyal-2 in L929 fibroblasts, BMC Cell Biol., 3, 8, 2002. 

In the early years primary cells were used as viral hosts. Although these have limited growth potential they support the replication of a wide variety of viruses. Continuous cell strains such as mouse L929 or human HeLa cells are now commonly used for virus growth in biochemistry laboratories, but for vaccine production untransformed, diploid cell strains are the preferred hosts. 

Fig. 4 Effect of treatment type on in vitro growth inhibition of ( ) specific cell-line, L929 expressing H2Kk protein, and ( ) non-specific cell-line, BALB/c3T6. The targeted and non-targeted liposomes were conjugated with anti-H2Kk IgG2A and anti-sheep erythrocyte IgG2A, respectively. (Adapted in part from Ref. l)...

L929 mouse fibroblast cells in this device changed their morphology due to the forces exerted on them. A gradient was created by introducing a standard culture medium with fetal bovine serum (FBS) at one inlet and a serum-free medium at the other. FBS contains proteins, such as serum albumin, that are needed for cell survival, division and growth. The cell density and attachment depended on a combination of sheer stress and FBS concentration [28]. 

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