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Procedure for Making Knockout Mice Using Embryonic Stem Cells

2b Procedure for Making Knockout Mice Using Embryonic Stem Cells. [Pg.21]

Embryonic stem cells (ES cells) and homologous recombination are utilized to inactivate an endogenous gene from a host s genome. ES cell lines are derived from a 3-day embryo (ICM cells) and are undifferentiated but remain totipotent. Mouse [Pg.21]

Introduce DNA into ES cello end select for cells thet heve integreted the DNA [Pg.22]


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