Klebsiella oxytoca

The hydrolysis with a (R)-specific amidase from Klebsiella oxytoca resolved 2-hydroxy- and 2-amino-3,3,3-trifluoro-2-methylpropionamide, giving the (R)-acid and the remaining (S)-amide (Figure 6.39) [104,105]. 

Patent literature reports on the analogous resolutions of phosphinotricin using, among others, penicillin G-acylase, penicillin G-amidase, subtilisin or microorganisms such as Enterobacter aerogenes, Klebsiella oxytoca, Corynebac-terium sp., Rhodococcus rubropertinctus and others7°... 

Hacisalihoglu A, JA Jongejan, JA Duine (1997) Distribution of amine oxidases and amine dehydrogenases in bacteria grown on primary amines and characterization of the amine oxidase from Klebsiella oxytoca. Microbiology (UK) 143 505-512. 

The deamination of primary amines such as phenylethylamine by Escherichia coli (Cooper et al. 1992) and Klebsiella oxytoca (Flacisalihoglu et al. 1997) is carried out by an oxidase. This contains copper and topaquinone (TPQ), which is produced from tyrosine by dioxygenation. TPQ is reduced to an aminoquinol that in the form of a Cu(l) radical reacts with O2 to form H2O2, Cu(ll), and the imine. The mechanism has been elucidated (Wihnot et al. 1999), and involves formation of a Schiff base followed by hydrolysis in reactions that are formally analogous to those involved in pyridoxal-mediated transamination. 

The catechol-type ligand appears to be restricted to siderochromes derived from prokaryotic microorganisms. Klebsiella oxytoca, an organism closely related to members of the genus Aerobacter, forms the 2,3-dihy-droxy-N-benzoyl derivates of serine and threonine in three day cultures (72). It is not known if the latter amino acid occurs in trimers but examination of space-filling CPK models does indicate that enterobactin could accomodate a methyl substituent on the carbon of the serine residue. Catechols occur in higher protist organisms but their formation... 

Figure 8.3 Preparation of (R)-2,2-HTFMPS and (S)-2,2-HTFMPA from racemic 3,3,3-trifluoro-2-hydroxy-2-methyl-propionamide using Klebsiella oxytoca PRS1...

Shaw, N.M., Naughton, A., Robins, K., et al. (2002) Selection, purification, characterisation, and cloning of a novel heat-stable stereo-specific amidase from Klebsiella oxytoca, and its application in the synthesis of enantiomerically pure (R)- and (S)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acids and (S)-3,3,3-trifluoro-2-hydroxy-2-methylpropionamide. Organic Process Research Development, 6, 497-504. 

Li J, Gu J-D (2006) Biodegradation of dimethyl terephthalate by Pasteurella mul-tocida Sa follows a novel biochemical pathway. Ecotoxicol 15 391-397 Li J, Gu J-D (2007) Complete degradation of dimethyl isophthalate requires the biochemical cooperation between Klebsiella oxytoca Sc and Methylobacte-rium mesophilium Sr isolated from wetland sediment. Sci Total Environ 380 181-187... 

Klebsiella oxytoca. Two French studies of patients with acute AAD used culture of colorectal biopsy to detect K. oxytoca, despite negative stool culture [19, 20], Colonoscopic findings included diffuse and focal colitis and right-sided hemorrhage colitis. The pathogenicity of these strains is still under debate. 

Benoit R, Dorval D, Loulergue J, Bacq Y, Oliver JM, Audurier A, Metman EH Post-antibiotic diarrheas Role of Klebsiella oxytoca (in French). Gastroenterol Clin Biol 1992 16 860-864. 

Activated sludge 4-Nitroaniline (an intermediate of dye), yeast extract Acinotobacter sp., Citrobacter freundii, Klebsiella oxytoca [14]... 

Known producers of CGTase include Bacillus mascerans (7), Bacillus megaterium (8), Bacillus ohbensis (9), alkalophilic Bacillus sp. (lO M), Bacillus amyloliquefacien (15), Bacillus subtilis (16), Klebsiella oxytoca (17), and Micrococcus sp. (18), However, none of these CGTase enzymes are sufficiently thermostable for use in industrial starch liquefaction. 

Condensation products of DHB (which usually is found also in the fermentation broth) with amino acids were reported, viz. with glycine ixom Bacillus subtilis (164) named subsequently itoic acid (282) with serine from Escherichia coli (261) and Klebsiella oxytoca (196) with threonine from Klebsiella oxytoca (196) and Rhizobium spp. (275, 327) with arginine from Pseudomonas stutzeri (62) with glycine and threonine from Rhizobium sp. (240) with threonine and lysine as well as with leucine and lysine from Azospirillum lipoferum (312, 320). In most cases the isolate (sometimes designated as being a siderophore) was hydrolyzed and the constituents were determined by paper chromatography. The relative amounts of the constituents, the chiralities of the amino acids and the molecular mass of the isolate have not been determined. Hence it is not known whether condensation products of the enterobactin type exist. 

Korth H (1970) Uber das Vorkommen von 2,3-Dihydroxybenzoesaure und Uuer Aminosau-rederivate in Kulturmedien von Klebsiella oxytoca. Arch Microbiol 70 297... 

Urinary tract infections Urinary tract infections (complicated and uncomplicated), including pyelonephritis and cystitis (initial and recurrent) caused by Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella oxytoca, Citrobacter sp., and Serratia marcescens. 

Following the first approach, Escherichia coli and Klebsiella oxytoca have been engineered to be highly effective ethanol produc-... 

Ohta, K., Beall, D. S., Mejia, J. R, Shanmugam, K. T., and Ingram, L. O., Metabolic engineering of Klebsiella oxytoca M5A1 for ethanol production from xylose and glucose. Appl Environ Microbiol 1991, 57 (10), 2810-5. 

Bothast, R. J., Saha, B. C., Flosenzier, A. V, and Ingram, L. O., Fermentation of L-arabinose, D-xylose and D-glucose by ethanologenic recombinant Klebsiella oxytoca strain P2. Biotechnology Letters 1994, 16 (4), 401-406. 

Candida famata Cryptococcus sp. UFMG-Y28 Cryptococcusflavus UFMG-Y61 Comamonas testosteroni ATCC 55744 Klebsiella oxytoca 38.1.2 Myrothecium verrucaria Pseudomonas chlororaphis B23 (PERM BP-187)... 

Ewert, C., Lutz-Wahl, S., and Fisher Lutz. 2008. Enantioselective conversion of a-arylnitriles hy Klebsiella oxytoca. Tetrahedron Asymmetry, 19 2573-8. 

Tobimatsu, T., Sakai, T., Hashida, Y., Mizoguchi, N., Miyoshi, S., and Toraya, T., 1997, Heterologous expression, purification, and properties of diol dehydratase, an adenosylcobalamin-dependent enzyme of Klebsiella oxytoca, Arch. Biochem. Biophys. 347 1320140. 

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