Myrothecium verrucaria

The question of the involvement of oxidative enzymes in the breakdown of cellulose has been raised periodically, but now there is definite evidence that such enzymes occur in some culture filtrates. This was established by Eriksson and his colleagues (2) when they found that concentrated cell-free filtrates of the fungi S. pulverulentum, Polyporus adustus, Myrothecium verrucaria, and T. viride solubilized cellulose at a slower rate under anaerobic than under aerobic conditions. 

Fungi present in Coniophora cerebella and Lenzites trabea 90 none in Aspergillus fumi-gatus, A. niger, A. oryzae, A. terreus,90 and Myrothecium verrucaria.°3... 

Myrothecium verrucaria and M. roridum,383 but neither [l,8-14C2]-(l jR,6i S)-a-bisabolol nor its (l S)-isomer was incorporated into the verrucarol skeleton,384 thus indicating that bisabolene derivatives are probably not intermediates in the formation of the trichothecane skeleton. The recent report of the occurrence of trichodiol (118) and of (119) has led to speculation385 that direct cyclization of 2-cis-6-trans-farnesol might be involved in the formation of the trichothecane skeleton the conversion of an intermediate such as trichodiene (120) into (118) should involve... 

Candida famata Cryptococcus sp. UFMG-Y28 Cryptococcusflavus UFMG-Y61 Comamonas testosteroni ATCC 55744 Klebsiella oxytoca 38.1.2 Myrothecium verrucaria Pseudomonas chlororaphis B23 (PERM BP-187)... 

Marier-Greiner, U.H., Obermaier-Skrobranek, B.M.M., Estermaier, L.M., et al. 1991. Isolation and properties of nitrile hydratase from the soil fungus Myrothecium verrucaria that is... 

Full details of the neat synthesis of the ketone (126) and its methylenation to give trichodiene (127) have been published.82 Unfortunately, sixteen attempts with different reagents to carry out a similar methylenation of the diastereoisomeric ketone (128) to give bazzanene (129) failed. A number of new and very interesting trichothecane sesquiterpenoids have been isolated from Myrothecium verrucaria. These include trichodermadienediol A (130), trichoverrol A (131), and trichoverrin A (132) together with their B counterparts which are epimeric at C-7. 83 The trichoverrins are clearly biosynthetic intermediates of the more toxic macrocyclic... 

Myrothecium verrucaria BOD derivatives, on glassy carbon electrode pH = 7.0, aeration of cell 0.3 0.03 ... 

Myrothecium verrucaria BOD Adsorption to carbon aerogel-modified 0 0.205 ry a... 

The use of highly specific enzymes was proposed to remove bilirubin from the bloodstream. More than 50 enzymes were tested, and an enzyme from Myrothecium verrucaria, bilirubin oxidase, which catalyzes the oxidation of bilirubin with Oz, was chosen. The initial product of the enzymatic reaction is biliverdin, which is much less toxic than bilirubin. The stability of bilirubin oxidase was greatly enhanced by immobilization At physiological temperature and pH, free bilirubin oxidase lost half of its activity in 12 h, whereas the half-life of the immobilized enzyme was 60 h. 

New trichoverroids from Myrothecium verrucaria Isolated by high speed countercurrent chromatography. J. JVfl/ Prod. 55,1441-1446. 

Figure 5. Relationship of change in number of end groups to change in chain length during random hydrolysis of carboxymethylcellulose by cellulase of Myrothecium verrucaria (23)...

However, cell-free enzyme preparations from many, according to the above definition, truly cellulolytic microorganisms fail to solubilize native cotton completely unless this is first converted into a reactive form by swelling in acidic or basic milieu (14,41). Thus, these organisms do not seem to produce a Ci enzyme. The most well-known example is culture filtrate from Myrothecium verrucaria (26, 55). 

Gel Filtration Technique. Cellulases were among the first proteins to be separated by the gel filtration technique. Cellulolytic enzymes from Polyporus versicolor were thus separated by Pettersson et ah (39) on Sephadex G-75 (Pharmacia Fine Chemicals, Uppsala, Sweden) and simultaneously Whitaker et ah (56) applied the same technique in studies on the cellulolytic enzymes from Myrothecium verrucaria. The gel filtration method has since been so frequently used in separation and... 

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