Interface ion spray

D. Puig, L. Silgoner, M. Grasserbauer and D. Barcelo, Part-per-trillion level determination of priority methyl-, nirto-, and clilor ophenols in river water samples by automated online liquid/solid exrtaction followed by liquid chr omatography/mass spectr ometry using atmospheric pressure chemical ionization and ion spray interfaces . Anal. Chem. 69 2756-2761 (1997). 

The ionspray (ISP, or pneumatically assisted electrospray) LC-MS interface offers all the benefits of electrospray ionisation with the additional advantages of accommodating a wide liquid flow range (up to 1 rnl.rnin ) and improved ion current stability [536]. In most LC-MS applications, one aims at introducing the highest possible flow-rate to the interface. While early ESI interfaces show best performance at 5-l() iLrnin, ion-spray interfaces are optimised for flow-rates between 50 and 200 xLmin 1. A gradient capillary HPLC system (320 xm i.d., 3-5 xLmin 1) is ideally suited for direct coupling to an electrospray mass spectrometer [537]. In sample-limited cases, nano-ISP interfaces are applied which can efficiently be operated at sub-p,Lmin 1 flow-rates [538,539]. These flow-rates are directly compatible with micro- and capillary HPLC systems, and with other separation techniques (CE, CEC). 

A.P. Bruins, T.R. Covey and J.D. Henion, Ion spray interface for combined liquid chromato graphy/atmospheric pressure ionization mass spectrometry, Anal. Chem., 59,2642 2646 (1987). 

Puig et al. [450] determined ng/1 levels of priority methyl-, nitro-, and chloro-phenols in river water samples by an automated on-line SPE technique, followed by liquid chromatography-mass spectrometry (LC-MS) using atmospheric pressure chemical ionization (APCI) and ion spray interfaces. 

Bruins, A.P. Covey, T.R. Henion, J.D. Ion Spray Interface for Combined Liquid Chromatography/Atmospheric Pressure lonization-MS. Anal. Chem. 1987, 59, 2642-2646. 

The resulting protocol of the coupling of HPLC with an MS/MS system, using an ion-spray interface, provides a means for sensitive and specific analysis of several BAs. Chromatographic separation allows the differentiation of isomeric forms. The MRM mode provides the necessary specificity even in the case of co-eluting components, thanks to the different transitions monitored this is evidenced by extracting the specific reconstructed ion chromatogram (RIC). The RIC traces of a BA standard mixture are shown in Fig. 5.4.13. 

The best answer I have heard for this question is deep pockets. You will need an ionizing interface and a mass spectrometer. The least expensive conversion that I know of was a customer who bought a slightly used GC/MS unit from a hospital lab for 50,000, pulled off the GC, bought an ion spray interface for 4,000, hooked it up to his HPLC, and got the instrument up and running. Normally, you would expect to pay at least twice that for a basic LC/MS, but prices show signs of coming down, so check around. 

Methyl, nitro and chlorophenols (EPA priority pollutants) LC-MS (atmospheric pressure chemical ionisation and ion spray interfaces) ppt [18]... 

ESI-MS/MS Electro spray ionization-tandem mass spectrometry TIS-MS/MS Turbo Ion Spray interface-tandem mass spectrometry EAB-MS Fast atom bombardment-mass spectrometry DCI-MS Desorption chemical ionization-mass spectrometry... 

Ion spray interface introduction (9 1) waste to MS split ESI interface Detection ... 

In order to ensure the column effluents are compatible with a mass selective system, a micromembrane suppressor for trapping nonvolatile ion-pairing agents used in LC-MS with a moving-belt interface is described by Escott et al. Both cationic and anionic membrane suppressors for LC-MS with an ion-spray interface were reported by Conboy et al., whereas Forngren et al. " removed nonvolatile mobile phase ingredients with an ion exchanger placed between the separation column and MS interface. 

The initial series of experiments assessed the stability of the conjugates to the pH and temperature conditions (37 °C) found in the mammalian GIT pH 1 and pH 9 were chosen to represent the normal extremes of acidity and basicity in the human. While pH 5 provided an intermediary value, it is also relevant to parts of the ruminant digestive system. The rate of degradation of the conjugates was monitored over a 24-hour period by LC-MS/MS (turbo ion spray interface). Optimization of the LC system provided an efficient method in which several compoimds could be analyzed simultaneously. During the early... 

Another way to speed up HPLC that does not interfere with the existing gradient separation method is by parallel operation of several HPLC columns. The development in this direction started a couple of years ago when fast gradient separation was first combined with mass spectrometry-based detection. Parallel column operation was achieved by a single pumping system and a splitter tee that transferred the gradient flow onto two HPLC columns. The effluent of the two columns was simultaneously sprayed into a modified ion spray interface of a quadrupole mass spec-trometer. From the overlay chromatogram both desired and previously known compounds were identified after their molecular ions were filtered from the total ion current (TIC). In this first system, however, it was difficult to enhance the parallelization, and the detection system created a bottleneck. ... 

Curini, R. et al.. Solid-phase extraction followed by high-performance liquid chromatography-ion spray interface-mass spectrometry for monitoring of herbicides in environmental water,... 

Detector MS, PE Sciex API 365 triple stage quadrupole LC-MS-MS, PE Sciex Turbo Ion Spray interface, positive ion mode, needle voltage 5.2 kV, nebulizer gas air at 60 psi, curtain gas nitrogen at 40 psi, collision cell gas nitrogen at 40 psi, turbo ionspray heater 375°, heater gas flow 7 L/min... 

Bruins, A. P. Covey, T. R. Henion, J. D. Ion spray interface for combined liquid chromatography/... 

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