Intraepithelial neoplasia tissues

Maitra A, Adsay NV, Argani P, et al. Multicomponent analysis of the pancreatic adenocarcinoma progression model using a pancreatic intraepithelial neoplasia tissue microarray. Mod Pathol. 2003 16 902-912. 

Goodwin AC, Jadallah S, Toubaji A, Lecksell K, Hicks JL, Kowalski J, Bova GS, De Marzo AM, Netto GJ, Casero RA Jr (2008) Increased spermine oxidase expression in human prostate cancer and prostatic intraepithelial neoplasia tissues. Prostate 68(7) 766-772. 

A recent smdy examined the level of DiAcSpm in colorectal cancer and intraepithelial neoplasia tissues (Kuwata et al. 2013). DiAcSpm was very scant in normal tissues but was markedly increased in resected stage II, HI, and IV colon cancer tissues. The level of DiAcSpm was also maikedly elevated in metastatic liver lesions in individuals with stage IV colon cancer compared to adjacent normal tissue. HPLC analysis indicated that the levels of Af-acetylspermidine and Af-acetylspermine are also increased in cancer tissues. This is the first report that definitively identifies the presence of DiAcSpm in human tissues. Importantly, DiAcSpm is markedly increased in tumor tissue compared to normal tissue. The levels of A/ -acetylspermidine and Af, V -diacetylspermidine were very low or undetectable in normal tissues and were not significantly increased in cancer tissues. 

The presence of human papilloma virus (HPV) is associated with female genital tract diseases such as condyloma, Bowenoid papulosis, and cervical, vaginal, and vulvar intraepithelial neoplasia and carcinoma. A general concern is the association of HPV with cervical cancer (Gl). The HPV consists of an icosahedral viral particle (virion) containing 8000 base pairs, a circular, double-stranded piece of DNA surrounded by a protein capsid. Viral replication takes place within the nuclei of infected squamous epithelial cells (H5). Following infection of epithelial cells, the viral DNA penetrates throughout the entire thickness of the epithelium, but intact viruses are found only in the upper layers of tissue. 

C34. Cope, G., Thorpe, G., Holder, R., Luesley, D., and Jordan, J., Serum and tissue antioxidant capacity in cervical intraepithelial neoplasia investigated using an enhanced chemiluminescent reaction. Am. Clin. Biochem. 36, 86-93 (1999). 

The mean number of microscopic blood vessels in tissue is higher in prostate cancer and prostatic intraepithelial neoplasia (PIN) than normal prostate tissue. In a study evaluating microvessel density (MVD) on needle biopsy the authors found MVD, when combined with Gleason score and preoperative PSA, provided improved ability to predict extraprostatic extension at radical prostatectomy. " Although microvessel density was significant in the multivariate analysis, Gleason score and serum PSA were much more powerful predictors of extraprostatic... 

The international Society for the Study of Vulvar Disease (ISSVD) introduced a new classification (1989) of vulvar intraepithelial neoplasia (VIN) terminology replacing previously used terms such as Bowen s disease, erythroplasia of Queyrat etc. VIN is a type of precancerous vulvar tissue abnormality. It is no longer considered as a problem of postmenopausal women, but can develop in any age. 

Figure 4.11 Raman spectra of normal cervical epithelial cells and invasive carcinoma cells (top), and (b) Raman spectra of cervical intraepithelial neoplasia (CIN) tissue (bottom). Assignments of the main Raman vibrational modes are detailed in Table 4.1.

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