Needle biopsy

Bioluminescence in vitro chemosensitivity assays are now used to assess the sensitivity of tumor cells (obtained by surgical or needle biopsy) to different dmgs and combinations of dmgs. Cells are grown in microwell plates in the presence of the dmgs at various concentrations. If the tumor cells are sensitive to the dmg then they do not grow, hence total extracted cellular ATP, measured using the bioluminescence firefly luciferase reaction, is low. This method has been used to optimize therapy for different soHd tumors and for leukemias (306). 

Transrectal prostate needle biopsy to be done if the patient has areas of nodularity or induration on digital rectal examination tissue biopsy can document the presence of prostate cancer, which can also cause enlargement of the prostate... 

Prostate needle biopsy Transrectally, a biopsy needle is inserted into the prostate. Tissue core is sent to a pathologist for analysis A normal prostate should have no evidence of BPH or prostate cancer The biopsy is consistent with BPH... 

Core Needle Biopsy of Right Breast Mass... 

Nordgren H, Nilsson S, Runn A-C, et al. Histopathological and immunohisto-chemical analysis of lung tumors description of a convenient technique for use with find needle biopsy. APMIS 1989 97 136-142. 

Pathologic confirmation of lung cancer is established by examination of sputum cytology and/or tumor biopsy by bronchoscopy, mediastinoscopy, percutaneous needle biopsy, or open-lung biopsy. 

The examinations are performed in real time and can visualize motion (30 frames per second or even more) respectively, needle biopsies and tissue ablation procedures are often performed under the guidance of ultrasound imaging. 

B17. Black, M., Billing, B. H., and Heirwegh, K. P. M., Determination of bilirubin UDP-glucuronyl transferase activity in needle-biopsy specimens of human liver. Clin. Chim. Ada 29, 27-35 (1970). 

Odifevre, M., and Luzeau, R., Measurement of the activity of bilirubin UDP-glucuronyl transferase. Application to needle biopsy specimens. Rev. Eur. Etud. Clin. Biol. 16, 84-85 (1971). 

Li Y, Ali S, Philip PA et al. Direct comparison of microarray gene expression profiles between nonamplification and a modified cDNA amplification procedure applicable for needle biopsy tissues. Cancer Detect Prev 2003 27 405 11. 

The in vitro system has some limitations and it can be used only as a model to address acquired drug resistance, because cell lines may not accurately reflect the in vivo situation of patients treated with anticancer drugs. In vivo analysis using patient samples to study anti-cancer drug resistance are described in the published literature. Chang and coworkers (64) showed that the gene-expression patterns from mRNA derived from needle biopsies of breast cancer patients treated with the anticancer drug, docetaxel, could be predictive of response to the therapy. 

A 53-year-old man developed unilateral gynecomastia following finasteride therapy for alopecia (75). On needle biopsy the mammary mass was diagnosed as... 

Jaundice and pruritus were observed at age 6 weeks and resolved spontaneously after approximately 2 months. He was hospitalized for pneumonia at age 20 months, and an enlarged liver was noted. A percutaneous needle biopsy specimen from the liver was interpreted to show postnecrotic cirrhosis, although reevaluation of the biopsy specimen showed the presence of globules that were periodic acid-Schiff (PAS) positive, diastase resistant (Fig. 4-1). He was then referred to our institution at age 2.5 years for liver transplantation. 

Buscarini, L., Fornari, F., Bolondi, L., Colombo, P., Livraghi, T., Mag-nolfi, F., Rapaedni, G.L., Sdmi, A. Ultrasound-guided fine-needle biopsy of focal liver lesions techniques, diagnostic accuracy and complications. A retrospective study on 2091 biopsies. J. Hepatol. 1990 11 344-348... 

Spamer, C., Brambs, H.-J., Koch, H.K., Gerok, W. Benign circumscribed lesions of the liver diagnosed by ultrasonically guided fine-needle biopsy. J. Clin. Ultrasound 1986 14 83-88... 

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