High Performance Liquid Chromatography-Mass Spectrometry

High performance liquid chromatography-mass spectrometry 

Volmer et al. [11] evaluated the performances of both thermospray and electrospray ion sources for determining trace levels of sulphonylurea herbicides in water. In terms of specificity, they concluded that the latter source, in combination with a tandem mass spectrometer, was superior to the former one. 

Further examples of the application of high performance liquid chromatography to the analysis of non saline waters are reviewed in Table 5.1. 

Barcelo [13] characterised selected pesticides by negative ion chemical ionisation thermospray high performance liquid chromatography-mass spectrometry. Ions observed 

High Performance Liquid Chromatography - Mass Spectrometry 

HPLC is a universal separation technique that is capable of separating both volatiles and non-volatiles without the need for derivatization. We are developing methods that employ both on-line photodiode array (PDA) detection and mass selective detection, HPLC/PDA/MS. This approach also utilizes an ion-trap mass 

HPLC/PDA/MS analysis of an alfalfa root extract. The HPLC retention time, the UV absorbance spectrum, and the mass spectrum readily identify the peak eluting at 45 minutes as medicarpin, a known phytoalexin in alfalfa. 

The utility of mass selective detection is greatest when analyzing compounds that do not contain chromophores or when structural information is needed for chemical identification. Triterpene saponins contain very weak chromophores and have long been associated with a variety of biological activities including 

Saponins isolated from the legume Acacia victoriae have been reported to trigger 

Applications of this technique are growing and include the analysis of mixtures of pesticides and herbicides, including organophosphorus, phenoxyacetic acid, carbamate, urea types, mixtures of various types of organic compounds, alkylbenzene sulphonates, polyethylene glycols, nonylphenyl ethoxylates, dioctadecylmethyl ammonium, ozonisation products and chlorination products. 

Based on a new technology, particle-beam enhanced liquid chromatography-mass spectrometry expands a chemist s ability to analyse a vast variety of substances. Electron impact spectra from the system are reproducible and can be searched against standard or custom libraries for positive compound identification. A simple adjustment to the particle-beam interface is all it takes. 

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In this book, I have tried to show the way in which high performance liquid chromatography-mass spectrometry (LC-MS) has developed, somewhat slowly it has to be said, into a powerful hybrid analytical technique. 

The Need for High Performance Liquid Chromatography-Mass Spectrometry... 

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