High-performance liquid chromatography column-packing equipment

Apparatus Use a suitable high-performance liquid chromatography system (see Chromatography, Appendix IIA) equipped with a dual wavelength detector system such that the effluent can be monitored serially at 254 nm and 325 to 385 nm (wide-band pass). Use a 1-m x 2.1-mm (id) column, or equivalent, packed with a strong anion-exchange resin (Dupont No. 830950405, or equivalent). 

Sugars. The high performance liquid chromatography (HPLC) method described by Hunt et al. (8) was used to determine the sugars in the kiwifruit and nectars with some modifications (21). A Waters Association Chromatograph equipped with a Model 6000-A solvent delivery system, a Model R401 refractometer detector, a D6K universal injector column was a 30 cm x 4 mm i.d. stainless steel tube packed with u-bondapak-carbohydrate (Waters Associates). The precolumn was packed with CO-PELL PAC (Whatman). The eluent was acetonitril and distilled water (85/15, v/v). 

High-performance liquid chromatography (HPLC) uses high pressure to force eluent through a closed column packed with micrometer-size particles that provide exquisite separations. The analytical HPLC equipment in Figure 22-14 uses columns... 

High performance liquid chromatography was performed with a high pressure liquid chromatography apparatus equipped with a U.V. detector (280 nm). The U.V. monitor has an 8 pi flow cell. The chromatographic column was a 20 cm x 2.1 mm (internal diameter) pre-packed stainless-steel Micro-Pack" column (Varian, Palo Alto, Calif.,... 

Procedure (See Chromatography, Appendix IIA.) Use a high-performance liquid chromatograph capable of separating acesulfame potassium and 4-hydroxybenzoic acid ethyl ester with a resolution of 2. Use a chromatograph equipped with a UV or diode array (227 nm) detector and a 25-cm x 4.6-mm (id) stainless steel column, or equivalent, packed with 3 to 5 p,m of reversed phase C18 silica gel, or equivalent. The elution is isocratic. Use a 40 60 (v/v) solution of acetoni-trile 0.01 MlL tetrabutyl ammonium hydrogen sulfate (TBAHS) in water as the mobile phase, with a flow rate of about 1 mL/min. 

Chromatographic System (See Chromatography, Appendix IIA.) Use a suitable high-performance liquid chromatograph equipped with a detector measuring at 210 nm and a 250- x 4.6-mm (id) column packed with octadecyl silanized silica (10-p.m Partisil ODS-3, or equivalent), and operated under isocratic conditions at 40°. The flow rate of the Mobile Phase is about 2 mL/min. 

Procedure (See Chromatography, Appendix BA.) Use a suitable high-performance liquid chromatograph equipped with differential refractometer, autosampler injection unit, mobile-phase degasser, column heating block or oven, and a computing integrator. The column is Lichrosorb RP-18 250-mm x 4.5 mm (id) (GL Science, Inc., or equivalent) and YMC-Pack ODA-A A-303 250-mm x 4.5 mm (id) (YMC Company, Ltd., or equivalent) connected in a series, or equivalent, maintained at 50°. Use 80 20 acetone acetonitrile as the eluent, at a flow rate of 2 mL/min. 

Procedure (See Chromatography, Appendix IIA.) Use a high-performance liquid chromatograph equipped with an ultraviolet detector that measures absorption at 254 nm and a 25- to 30-cm x 4-mm (id) stainless-steel column, or equivalent, packed with octadecyl silane chemically bonded to porous silica or ceramic microparticles 5 to 10 pun in diameter, or equivalent. Maintain the mobile phase at a pressure and flow rate capable of giving the required resolution (see below). Inject a volume, up to 25 pL, of the System Suitability Solution in a similar manner. Calculate the resolution, R (>3.6), between calcium formyltetrahydrofolate and Folic Acid by the equation... 

Procedure Use a high-performance liquid chromatograph (see Chromatography, Appendix IIA) equipped with an ultraviolet detector that measures at 254 nm. Under typical conditions, the instrument contains a 300- x 4-mm (id) column packed with 10-p.m octadecylsilanized silica (jxBon-dapak C 18, or equivalent). Set the flow rate at about... 

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