High performance liquid chromatography packing columns

Williams, R.C., Vasta-Russell, J.F., Glajch, J.L., and Golebiowski, K., Separation of proteins on a polymeric fluorocarbon high-performance liquid chromatography column packing, J. Chromatogr., 371, 63, 1986. 

Melo, L.F.C. Collins, C.H. Collins, K.E. Jardin, I.C.S.F. Stability of high performance liquid chromatography columns packed with poly(methyloctylsiloxane) sorbed and radiation-immobilized onto porous silica and zirconized silica. J. Chromatogr., A 2000, 869, 129-135. 

JJ Kirkland, JL Galjch, RD Farlee. Synthesis and characterization of highly stable bonded phases for high-performance liquid chromatography column packings. Anal Chem 61 2—11, 1988. 

Source From Stability of high performance liquid chromatography columns packed with poly(methyloctylsiloxane) sorbed and radiation-immobilized onto porous silica, and zirconized silica in J. Chromatogr. 

Wang, T., R. Hartwick, N. T. Miller, and D. C. Shelly, Packing of Preparative High-Performance Liquid Chromatography Columns by Sedimentation, J. Chromatogr. 523, 23-34 (1990). 

Unfortunately, most column and sorbent manufacturers do not develop column packing materials mainly for SEC work, but for the bigger high-performance liquid chromatography (HPEC) market. However, there are many important differences to consider when designing packings for different modes of chroma-... 

Reversed-phase high-performance liquid chromatography (HPLC) column 50 mm x 3.2-mm i.d. with Kromasil 5- um Gig packing High-performance liquid chromatograph coupled to a triple-quadrupole mass spectrometer with an atmospheric pressure chemical ionization (APCI) source Gel permeation chromatograph with a 60 mm x 25-mm i.d. column packed with Bio-Beads SX-3 (50-g)... 

Tsuda, T. and Novotny, M., Packed microcapillary columns in high performance liquid chromatography, Anal. Chem., 50, 271, 1978. 

Gusev, I., Huang, X., Horvath, C. (1999). Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography. J. Chromatogr. A 855, 273-290. 

Kohne, A.P., Welsch, T. (1999). Coupling of a microbore column with a column packed with non-porous particles for fast comprehensive two-dimensional high-performance liquid chromatography. J. Chromatogr. A 845, 463-469. 

Nimura, N., Itoh, H., Kinoshita, T., Nagae, N., Nomura, M. (1991). Fast protein separation by reversed-phase high-performance liquid-chromatography on octadecylsilyl-bonded non-porous silica-gel — effect of particle-size of column packing on column efficiency. J. Chromatogr. 585(2), 207-211. 

Particulate sorbents are available almost exclusively in the shape of micrometersized beads. These beads are packed in columns and represent currently the most common stationary phases for high-performance liquid chromatography (HPLC). Despite their immense popularity, slow diffusional mass transfer of macromolecular solutes into the stagnant pool of the mobile phase present in the pores of the separation medium and the large void volume between the packed particles are considered to be major problems in the HPLC of macromolecules, frequently impairing their rapid and efficient separation [1]. 

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