Stainless-steel columns

Stainless steel column coated with y -bis(y -phenoxyphenoxy)benzene. 

DichIoro-l,3-butadiene [1653-19-6] M 123.0, b 41-43 /85mm, 98 /760mm. Crystd from pentane to constant melting point about -40°. A mixture of meso and d,l forms was separated by gas chromatography on an 8m stainless steel column (8mm i.d.) with 20% DECS (diethyleneglycolsilyl chloride) on Chromosorb W (60-80 mesh) at 60° and 80mL He/min. [Su and Ache J Phys Chem 80 659 1976.]... 

Gas chromatographic analysis at 79° using a flame detector in conjunction with a 183 x 0.32 cm. stainless-steel column containing Dow-Corning 550 fluid on silanized support gave peaks for l-bromo-3-chloropropane (6.5 minutes) and 6-chloro-2-hexyne (9.3 minutes) whose areas were shown to be proportional to the mole fractions. The latter were determined by integration of the expanded (50 Hz sweep width)... 

BioProcess stainless-steel columns are fixed bed height columns designed for the most stringent requirements in the routine production of biopharmaceuticals. Wetted materials include stainless steel, polypropylene, and EPDM. The BPSS series may be operated at pressures up to 3 bar (0.3 MPa) and are supplied with sanitary fittings of 10 or 22 mm i.d. The available column sizes and specifications for the BPSS column series are given in Table 2.18. 

Also available are semipreparative, 21.5-mm i.d. and preparative 55-, 108-, 158-, and 210-mm i.d. stainless-steel columns packed with TSK-GEL SW parti-... 

SynChropak GPC and CATSEC columns are packed in either stainless steel or PEEK columns with standard inverted fittings so that they readily connect to most instruments. Stainless-steel columns are available in 2.1, 4.6, 7.8, 10,... 

Columns need to withstand a pressure of at least several thousand pounds per square inch. Stainless-steel columns are recommended. 

Hotter and Balannec published one of the first real proposals to use SMB as a production tool for the pharmaceutical industry, and thus to scale down a process already used on a production scale [29]. The first commercially available plant (Licosep) SMB system was offered by Separex in 1991 and was exhibited for the first time in June 1991 during the Achema Exhibition. The system consisted of 24 stainless steel columns with adjustable lengths between a few centimeters up to almost 1 meter, HPLC pumps, and mulitpositional valves. To improve the robustness of the system, a rotary valve replaced two-way valves and the pumps were modified. 

A stainless steel column (4.6 mm internal diameter by 250 mm length) packed with 7 micron Zorbax ODS (Dupont) was equilibrated with 82 % Acetonitrile in water at a flow rate of 2.0 ml/min. provided by a Spectra Physics Model 87(X) pump and controller. The effluent was monitored at 230 nm using either a Tracor UV-Visible detector Model 970A or a Jasco Uvidec UV detector Model 1(X)-V. Peaks were recorded and calculated on a SpectraPhysics recording integrator. Model 4200 or Model 4270. Samples of 0.5 mg/ml in toluene were applied to the column automatically with a Micromeritics Autosampler Model 725 equipped with a 10 pi loop. 

Samples were fractionated by size on stainless steel columns packed with TSK-GEL ToyoPearl gel filtration medium, (Supelco Inc., Bellefonte, PA) in 50 mM NH4AC, pH 5.2. For HW-55 S (fractionation range for dextrans 1000-200,000 daltons) and HW-50 S (fractionation range for dextrans 500-20,000 daltons), the column was 10 x 500 mm and the flow rate was 1 ml/min. For HW-40 S (fractionation range for dextrans 100-7000 daltons),... 

The experimental set-up used for porous chromatography is virtually identical, to that used for HDC as described elsewhere (i6,1T). The use of stainless steel columns for the LEG work required 3l6 stainless steel column end-fittings and l/l6 O.D. capillary tubing. 

Three column sets were used in this study. All consisted of 0.95 X 122 cm stainless steel columns slurry packed with Control-led-Pore Glass from Electronucleonics. The configurations were ... 

Stainless-steel column, 4-mm i.d., 250-mm length Zorbax BP ODS Acetonitrile-water (3 2, v/v) l.OmLmin ... 

The 1/16" x 0.02" i.d. transfer line also functioned as a sample dilution device in other applications, a stainless steel column packed with glass beads has been found to be useful for dilution. This simple dynamic dilution technique has been used extensively in flow injection analysis.3 A refractive index detector is typically used to measure the sample transfer time. As shown in Figure 4, approximately 5 minutes is required to transfer the sample plug to the Rheodyne valve. As the apex of the sample band passes though the Rheodyne valve, the valve is activated and 1 pi injected onto the liquid chromatographic column. The sample transfer time was checked periodically over 1 year of operation and found to be stable. 

Format Stainless steel column Plastic cartridge... 

The chromatographic procedure may be carried out using (a) a stainless steel column (10 cm x 4.6 mm) packed with stationary phase C (3 pm) (Hypersil ODS in suitable), (b) as the mobile phase with a flow rate of 2 mL/min a 0.6% (w/v) solution of ammonium acetate in a mixture of 300 volumes of acetonitrile, 320 volumes of methanol and 380 volumes of water, and (c) a detection wavelength of 235 nm. 

Stainless steel column (25 cm x 4.6 mm) of YWG-C18H37 (10 pm) Aqueous 85% methanol [1.5 mL/min] 267 nm Reversed-phase HPLC analysis in rabbit serum. [97]... 

Zsolnay and Kiel [26] have used flow calorimetry to determine total hydrocarbons in seawater. In this method the seawater (1 litre) was extracted with trichlorotrifluoroethane (10 ml) and the extract was concentrated, first in a vacuum desiccator, then with a stream of nitrogen to 10 pi A 50 pi portion of this solution was injected into a stainless steel column (5 cm x 1.8 mm) packed with silica gel (0.063-0.2 mm) deactivated with 10% of water. Elution was effected, under pressure of helium, with trichlorotrifluoroethane at 5.2 ml per hour and the eluate passed through the calorimeter. In this the solution flowed over a reference thermistor and thence over a detector thermistor. The latter was embedded in porous glass beads on which the solutes were adsorbed with evolution of heat. The difference in temperature between the two thermistors was recorded. The area of the desorption peak was proportional to the amount of solute present. 

Solvent delivery system stainless steel columns injection port flow-through detector, recorder. Applications... 

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