Hela-S3 cells

Amino-2 -deoxypurines. 2 -AmiQo-2 -deoxyadenosine (15) is a naturally occurring A[-nucleoside isolated from A.ctinomadura that shows antknycoplasmal activity (1,4). Adenosine is the direct precursor for its biosynthesis (30). 2 -Arnino-2 -deoxyguanosine (16), isolated from a strain of Enterobacter cloacae (1,4), shows the growth of HeLa S3 cells and Sarcoma 180 in vivo and has been tested for antibacterial activity. 

Pyrene is metabolized by the fungus Crinipellis stipitaria to 1-hydroxypyrene, and this has a spectrum of toxic effects substantially greater than those of pyrene these include cytotoxicity to HeLa S3 cells, toxicity to a number of bacteria and to the nematode Cae-norhabditis elegans (Lambert et al. 1995). 

DIH, DNA single-strand breaks, HeLa S3 cells in vitro + NT 21 Painter (1978)... 

The macrolide glycosides, aurisides A (17) and B (18), from the Japanese sea hare Dolabella auricularia show cytotoxicity against HeLa S3 cells with IC50 values of 0.17 and 1.2 pg/ml, respectively [35]. 

The bromotriterpene aurilol (19) also was isolated from the sea hare Dolabella auricularia. Aurilol exhibits cytotoxicity against HeLa S3 cells with an IC50 of 4.3 pg/ml [36]. 

Dolabellin (169), a bisthiazole metabolite from the Japanese sea hare Dolabella auricularia containing 7,7-dichloro-octanoic acid, shows cytotoxicity against HeLa S3 cells (IC5o 6.1 pg/ml). The dechloro analogue has only one third of the toxicity [130]. 

Toxic to HeLa-S3 cells in vitro no in vivo antitumor activity 360... 

Active against HeLa and HeLa-S3 cells in vitro, against Ehrlich ascites and SI80 cells in vivo (mice) antibacterial toward gram-positive species 360... 

The title substituted 6 phenylpurine bases and nucleosides 10—13, as well as some acetyl derivatives 8, were tested on their in vitro inhibition of the cell growth in the following cell cultures mouse leukemia L1210 cells (ATCC CCL 219) murine L929 cells (ATCC CCL 1) human cervix carcinoma HeLa S3 cells (ATCC CCL 2.2) and human T lymphoblastoid CCRF-CEM cell line (ATCC CCL 119). Only substituted 6-phenylpurine ribonucleosides 12 and their triacetates 8 exhibited significant activity in these assays (Table 1), while the bases 10 and 11, as well as the 2 amino 6 phenylpurine ribonucleosides 13, were entirely inactive. 

Human cervix carcinoma HeLa S3 cells (ATCC CCL 2.2)... 

We then examined the effect of phospholipid composition on the transfection activity. Liposomes containing various combinations of phospholipids were tested for transfection activity on BHK-21 and HeLa-S3 cells. As described in HVJ-AVE liposomes, the cationic liposomes containing all of ePC, DOPE, and eSph in equal molar amounts showed the highest transfection efficiency both with BHK-21 and HeLa-S3 cells. The same results were obtained with Ltk-, HEK 293, and NB-1 cells. We also examined other phospholipids, but none was observed to be more effective. We then examined the effect of the cholesterol/phospholipid ratio on the transfection efficiency. The phospholipid composition (ePC DOPE eSph = 1 1 1) and DC-Chol content (10% of total... 

Attardi and Ching (1979) describe a method which has been used to reproducibly isolate mitochondria from cultured cells. Their method originally used Hela S3 cells growing in suspension ... 

Fig. 10.9. Accumulation of mitotic cells. The mitotic collection function (log 1 + Nm) is plotted against time for Chinese hamster cells (T = 12.4 h) and HeLa S3 cells ( T — 20.1 h) to which colcemid was added at zero time. (Reproduced from Puck, 1964, with kind permission of the author and publisher.)...

Fig. 10.10. Accumulation of labelled mitotic cells. A similar experiment with HeLa S3 cells to that shown in Fig. 10.9 except that [3H]thymidine was added with the colcemid at zero time. After an initial lag the lines for accumulation of all mitotic cells and labelled mitotic cells are parallel and separated by tG2. (Reproduced from Puck and Steffen, 1963, with kind permission of the authors and publisher.)...

Human (HeLa S3 cells) Unscheduled DNA synthesis — — Barrett 1985... 

BarrettRH. 1985. Assays for unscheduled DNA synthesis in HeLa S3 cells. In Ashby J, de Serres FJ, Draper M, et al., eds. Progress in mutation research 5. Evaluation of short-term tests for carcinogens Report of the International Program on Chemical Safety s collaborative study on in vitro assays. Amsterdam, Netherlands Elsevier Science Publisher, 347-352. 

Topoisomerase I was partially purified from the nuclei of HeLa S3 cells by a procedure that involved lysing the nuclei and precipitating nucleic acid with polyethylene glycol. 

Azahypoxanthine prevented cloning of human malignant HeLa S3 cells, but not of normal human cells, whereas 8-azaadenine prevented cloning of either type. °° 8-Azainosine (1 fiM) inhibited colony formation by human epidermoid (type 2) carcinoma cells in culture. In cell-free extracts of HeLa cells, protein synthesis was inhibited by the incorporation of 8-aza-guanine into the mRNA of polysomes, in which it blocked formation of peptide bonds. ... 

Chisholm PM, Danpure HJ, Healey G, Osman S (1979) Cell damage resulting from the labelling of rat lymphocytes and HeLa S3 cells with In-lll-oxine. J Nucl Med 20 1308-1311 Danpure HJ, Osman S, Carroll MJ (1988) The development of a clinical protocol for the radiolabelling of mixed leukocytes with " Tc-hexamethylpropyleneamine oxime. Nucl Med Commun 9 465-475... 

The sea hare Dolabella auricularia (Aplysiidae) has been extensively investigated as a rich source of bioactive natural products, and a number of bioactive peptides and depsipeptides such as dolastatin 10 were isolated from both Western Indian Ocean specimens and Japanese specimens of this animal [for example, 134, 135]. This mollusk was also found to contain macrolide-type natural products. From D. auricularia, collected by hand at a depth of 0-1 m off the coast of the Shima Peninsula, Mie Prefecture, Japan, a 22-membered macrolide, dolabe-lide A (65), was isolated as a cytotoxin with an IC50 value of 6.3 pg/ml against HeLa-S3 cells. The gross structure of 65 was determined by spectroscopic analysis, and its absolute stereochemistry was elucidated by a combination of chemical means and the NMR spectroscopic method [136]. 

Hela-S3 cell <6> (<6> uridine kinases mRNA expression is higher than in normal tissue [17]) [17]... 

Crystal violet staining uses lethal cytotoxicity a.s the end point (Itagaki et al., 1991), Cultured HeLa S3 cells or SlRC cells (an established line of rabbit corneal epithelial cells) are incubated with serial dilutions of the lest substance, and then crystal violet is used to stain residual viable cells. The... 

Among the naphthalenoid ansamycin antibiotics, the streptovaricin complex was reported to inhibit focus formation by MSV (MLV murine leukaemia virions) and to inhibit the splenomegaly-induced Rauscher leukaemia by virus selectively [226,227]. On the other hand, awamycin (26) [34,35] was shown to possess activity against HeLa S3 cells and antitumor activity against experimental murine tumors. Antitumor activity tests on streptovaricin C (44) were conducted [226,227]. 

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