Crystal violet stain

Gram positive Bacterial cells which retain the crystal violet stain during a staining procedure. Most strains of bacilli are gram positive. 

Fig. 10.19 Lack of toxic effects of CM fullerene on breast epithelial cells. Cm does not inhibit cell proliferation. MCF 10A and (A) MDA MB 231 (B) breast cancer cell lines were cultured either in the absence or presence of methanol C60 and cell proliferation was assayed by crystal violet staining. Control, no C 1 Opg Cm, A 50 pg Cm, X 250 pg CM. (C). MDA MB 231 cells were simultaneously stained with calcein and ethidium using a live-dead assay kit. Lack of red-colored cells and the presence of cells stained in green indicate the lack of toxicity (D). MDA MB 231 cells were either untreated (open box) cultured with varying amounts 10 (gray ), 50 (patterned ) and lOOpg (filled ) of C60 for 48 h and analyzed for cell cycle progression by flow cytometry (Levi et al., 2006) (See Color Plates)...

Fig. 4. CHO cells on a microcarrier a optical microscopy (contrast enhancement by Crystal Violet staining) and b scanning electron microscopy...

Fig. 2.2. Positive and negative adhesion of fibroblasts on fibronectin and tenascin coating of a tissue culture dish, after saturation with BSA. After I h of plating in serum-free medium, cells appear loosely attached on BSA, well spread on fibronectin and not attached on tenascin. Crystal violet staining. (Photograph courtesy of Ruth Chiquet, Friedrich Miescher Institute.)...

Use of magnification (e.g., a microfiche reader) makes reading crystal violet-stained plaques easier. 

The thickness and chemical composition of the cell wall and its adjacent structures determine how avidly a cell wall takes up and/or retains specific dyes. Most cells can be differentiated on the basis of whether they retain crystal violet stain during the Gram stain procedure. Those that can retain the dye, called Gram positive, do so because their cell walls consist of a thick peptidoglycan layer. In contrast, Gram-negative cells possess a thin peptidoglycan layer. Often... 

Crystal violet staining uses lethal cytotoxicity a.s the end point (Itagaki et al., 1991), Cultured HeLa S3 cells or SlRC cells (an established line of rabbit corneal epithelial cells) are incubated with serial dilutions of the lest substance, and then crystal violet is used to stain residual viable cells. The... 

Itagaki, H., Hagino, S Kaio, S Kobayashi. T., and Umeda, M. (1991). An in vitro alternative to the Draize eye iiriiation test Evahiation of the crystal violet staining method. Toxicol in Vitm 5, 139-143. 

Quadriceps muscle biopsy histochemistry showed moderate recent denervation and prominent established reinnervation. No amyloid was seen by crystal violet staining or with the Askanas Congo red fluorescence (see Chapter 7). 

Among several of our patients with other non-IBM vacuolar myopathies, including acid-maltase deficiency, hypokalemic periodic paralysis, myofibrillar myopathy, and undefined types, none had true amyloid deposits as identified by crystal violet staining. Abnormal muscle fibers in myofibrillar myopathy (as originally reported by De Bleecker et al. [9]) indeed have fluorescence-enhanced congophilic accumulations, but we doubt that in... 

Crystal violet is an excellent stain that is widely used in cytology, histology, and bacteriology. It is a typical basic dye that can be dissolved in both water and alcohol, allowing the staining of cell nuclei. Crystal violet staining is often used in the evaluation of cytotoxicity and growth inhibition assays. 

Saotome, K., Morita, H., and Umeda, M. 1989. Cytotoxicity test with simplified crystal violet staining method using microtitre plates and its application to injection-drugs. Toxicology in Vitro, 3 317-21. 

Gram-positive bacteria are those that are stained dark blue or violet by Gram staining. This is in contrast to gram-negative bacteria, which cannot retain the crystal violet stain, instead taking up the counterstain (safranin or fuchsine) and appearing red or pink. 

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