Hexamethylpropyleneamine oxime

Tc-Liposome-Labeling Method Using Hexamethylpropyleneamine Oxime-Glutathione... 

One method for the labeling of liposomes with chelator, hexamethylpropyleneamine oxime (HMPAO) was developed by Phillips et al. (16). Lipophilic HMPAO enters the liposome where it interacts with glutathione and becomes converted to the hydrophilic form, which is trapped in the liposome. A detailed protocol for radiolabeling liposomes using Tc-HMPAO has been reported (3). In a typical experiment, 10 to 15mCi (370-555 MBq) of Tc in the form of sodium pertechnetate... 

Figure 1 Images of rats with unilateral Staphylococcus aureus abscess in calf muscle, recorded at 0 (live minutes), 1,6, and 24 hours postinjection of Tc-labeled hydrazino nicotinamide-liposomes (A) and hexamethylpropyleneamine oxime-liposomes (B).

Perfusion hexamethylpropyleneamine oxime (HMPAO) single-photon emission computed tomography (SPECT) or 2-[ F]fluoro-2-deoxy-D-glucose positron emission tomography (FDG PET) can be used to help to differentiate between Alzheimer s disease, vascular dementia and frontotemporal dementia if the diagnosis is in doubt. 

Becker W, Borner W, Borst U (1988 a) " Tc-hexamethylpropyleneamine-oxime (HMPAO) as a platelet label evaluation of labelling parameters and first in vivo results. Nucl Med Commtm 9 831-842... 

Chisholm PM, Danpure HJ, Healey G, Osman S (1979) Cell damage resulting from the labelling of rat lymphocytes and HeLa S3 cells with In-lll-oxine. J Nucl Med 20 1308-1311 Danpure HJ, Osman S, Carroll MJ (1988) The development of a clinical protocol for the radiolabelling of mixed leukocytes with " Tc-hexamethylpropyleneamine oxime. Nucl Med Commun 9 465-475... 

Hexamethylpropyleneamine oxime (HMPAO) stereoisomers and their technetium-99m complexes were resolved on a 40°C Chiralcel OD column [614]. The d- and /-uncomplexed isomers were separated in 20 min using a 97/3 hexane/IPA (0.01% diethylamine) mobile phase. The ""Tc complexes were also resolved but with an 85/15 hexane/IPA mobile phase. Temperature increases from 20°C to 40°C greatly improved peak shape and resolution. A table of resolution for the Tc complexes of meso-, d- and /-HMPAO was generated for 90/10 to 0/100 hexane/IPA mobile phases. Retention times for 65/35 to 85/15 hexane/IPA were also tabulated. For all these mobile phases the retention times were under 15 min. 

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