Fibrous-bed bioreactor

Continuous Production of Butanol by Clostridium acetobutylicum Immobilized in a Fibrous Bed Bioreactor... 

We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35°C. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the solventogenesis phase, and the optimal butanol productivity of 4.6 g/(L h) and a yield of 0.42 g/g were obtained at a dilution rate of 0.9 h1 and pH 4.3. Compared to the conventional acetone-butanol-ethanol fermentation, the new fermentation process greatly improved butanol yield, making butanol production from corn an attractive alternative to ethanol fermentation. 

Index Entries ABE fermentation butanol Clostridium acetobutylicum fibrous bed bioreactor dilution rate. 

The present study on butanol fermentation has been focused primarily on the effects of pH and dilution rate (D) in continuous cultures of the mutant strain from C. acetobutylicum ATCC 55025. To overcome the problems of low productivity and yield of butanol, cell immobilization in a convoluted fibrous bed bioreactor (FBB) and feeding with dextrose and butyric acid as cosubstrates to produce butanol and reduce production of ancillary byproducts were used in the fermentation. By changing the dilution rate from 0.1 to 1.2 h 1 at pH 4.3 and varying the pH from 3.5 to 5.5 at the dilution rate of 0.6 hr1, the optimal conditions for high productivity and butanol yield were investigated. 

Luo, J. Three Dimensional Culturing of Animal Cells in Fibrous Bed Bioreactor. Ph.D. Thesis, The Ohio State University, 2002. 

Chen, C. Huang, Y.L. Yang, S.-T. A fibrous bed bioreactor for continuous production of developmental endothelial Locus-1 by osteosarcoma cells. J. Biotechnol. 2002, 97, 23-29. 

Shim H., Yang S.T. 1999. Biodegradation of benzene, toluene. Ethylbenzene, and o-xylene by a coculture of Pseudomonas putida and Pseudomonas fluorescens immobilized in a fibrous-bed bioreactor, J. Biotechnol., 67, 99-112... 

Yang ST, Luo J, Chen C (2004) A fibrous-bed bioreactor of continuous production of monoclonal antibody by hybridoma. Adv Biochem Eng Biotechnol 87 61-%... 

Recently Lan et al. (2013) smdied the production of cellulolytic enzymes by Trichoderma viride in a rotating fibrous-bed bioreactor (RFBB) and compared its performance with that of an STR. It was envisaged that the immobilization of T. viride mycelia on solid support of polypropylene cloth would eliminate some of the difficulties associated with the use of STRs (such as disintegration of the mycelia by the impeller, fouling of the pH and temperature probes, and clogging of the sample... 

Lan TQ, Wei D, Yang ST, LiuX. (2013). Enhanced ceUulase production by Trichoderma viride inarotating fibrous bed bioreactor. Bioresour Technol, 133, 175-182. 

Various PA fermentation methods have been attempted. The most common approaches are batch and fed-batch culture, which have significantly improved PA production over the past few decades. Maximum PA (44.62 g/L) was produced by P. acidipropionici at 220 h in fed-batch fermentation, which is limited by feedback inhibition of PA and the high accumulation of by-produas. Extractive fermentation and cell immobilization have been developed to reduce end product inhibition, which has significantly enhanced PA production. Until now, the highest PA produaion reached 136 g/L, which was obtained from constant, fed-batch fermentation in a plant fibrous bed bioreactor with immobilized P.freudenreichii. Due to the drawbacks of extractive... 

Talabardon M, Schwitzguebel JP, Peringer P.Yang ST. Acetic acid production from lactose by an anaerobic thermophilic coculture immobilized in a fibrous-bed bioreactor. Biotechnol Progr 2000 16 1008-17. 

Suwannakham S.Yang ST. Enhanced propionic acid fermentation by Propionibacterium acidipropionici mutant obtained by adaptation in a fibrous-bed bioreactor. Biotechnol Bioeng 2005 91 325-37. 

Chen F, Feng XH, Xu H, Zhang D, Ouyang PK. Propionic acid production in a plant fibrous-bed bioreactor with immobilized Propionibacterium freudenreichii CCTCC M207015. J Biotechnol 2012 164 202-10. 

Feng XH, Chen F, Xu H, Wu B, Yao J, Ying HJ, Ouyang PK (2010) Propionic acid fermentation by Propionibacterium freudenreichii CCTCC M207015 with a fibrous-bed bioreactor. Bioprocess Biosyst Eng 24 1077-1085... 

Zhu, H. and Yang, S.T. (2004) Longterm continuous production of monoclonal antibody by hybridoma ceiis immobilized in a fibrous-bed bioreactor. Cytotechnology, 44, 1 — 14. 

Two processes for production of 24 t/day of CMA from 10 lbs of cheese whey permeate per day utilizing the fibrous bed bioreactors were evaluated for product quality and manufacturing costs (Yang et al. 1999). O Figure 1.8 diagrams both processes. 

Liang Z-X, Li L, Li S, Cai Y-H, Yang S-T, Wang J-F (2012) Enhanced propionic acid production from Jerusalem artichoke hydrolysate by immobilized Propionibacterium acidipropionici in a fibrous-bed bioreactor. Bioprocess Biosyst Eng 35(6) 915-921. doi 10.1007/s00449-011-... 

Huang WC, Ramey DE, Yang ST (2004) Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor. Appl Biochem Biotechnol 113-116 887-98... 

Taskin M, Esim N, Ortucu S (2012) Efficient production of L-LA from chicken feather protein hydrolysate and sugar beet molasses by the newly isolated Rhizopus oryzae TS-61. Food Bioprod Process 90 773-779. doi 10.1016/j.fbp.2012.05.003 Tay A, Yang ST (2002) Production of L(+)-LA from glucose and starch by immobilised cell of Rhizopus oryzae in a rotating fibrous bed bioreactor. Biotechnol Bioeng 80 1-12. doi 10.1002/ bit. 10340... 

Butyric acid Immobilized Clostridium tyrobutyricum Cane molasses Fed-batch fermentation in fibrous bed bioreactor 55.2 0.47g/g 1.45 Jiang et al. (2009a)... 

Huang, J., Cai, J., Wang, ]., Zhu, X., Huang, L., Yang, S.T., Xu, Z., 2011b. Efficient production of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor. Bioresource Technology 102 (4), 3923-3926. 

Jiang, L., Wang, J., liang, S., Wang, X., Cen, P., Xu, Z., 2009a. Butyric acid fermentation in a fibrous bed bioreactor with immobUized Clostridium tyrobutyricum from cane molasses. Bioresource Technology 100 (13), 3403-3409. 

Lu, C., Zhao, ]., Yang, S.-T., Wei, D., 2012. Fed-batch fermentation for n-butanol production from cassava bagasse hydrolysate in a fibrous bed bioreactor with continuous gas stripping. Bioresource Technology 104,380-387. 

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