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Factor analytical techniques matrices

Since the rank of Y can be estimated by factor-analytical technique with consideration of experimental noise, the number of unexpected interferents, M, can be obtained easily by subtracting N from the rank of Y. The information on the number of interferents is crucial in this situation, this makes the distinction between matrix calibration and vector calibration. Assuming the bilinear structure of the response, one can factor-decompose the overall background responses of M interferents into the product of two matrices... [Pg.74]

The sample workup necessary for pesticide residue analysis will vary with each combination of analyte and antibody, each of which may have a different tolerance for the matrix and other factors. The effects of these factors must be considered as with the development of any other analytical technique. Matrix effects for one ELISA system are summarized in Figure 4. While the effect of the matrix on the antibodies in Figure 4 is different for each antibody-solvent-matrix combination, the competitive ELISA standard curves for most of these combinations are similar when expressed as percent of the appropriate control. Some systems may not require extensive adjustment, but this must be tested with each individual system. For example, our molinate assay performs equally well in a variety of water types at high concentrations of molinate (Figure 5). The small difference seen between the buffer and water standard curves in Figure 5 was eliminated by the addition of small amounts of concentrated buffer to water samples to equalize them to the buffer composition. [Pg.315]

Factors to consider in selecting a suitable extraction procedure are (a) the analytical information required, (b) the nature of the food matrix, (c) the form in which the vitamin occurs naturally or is added, (d) the nature and relative amounts of potentially interfering substances, (e) the stability of the vitamin in heat and extremes of pH, and (f) the selectivity and specificity of the analytical technique to be used. [Pg.337]

As with any analytical technique, generation of a reproducible standard curve with minimal error is critical. An assay calibration consists of several steps during which the value of the primary standard is transferred to the calibrators used in the final assay [22]. Immunoassay optimization is usually difficult due to protein heterogeneity and matrix effects and these factors, heterogeneity and matrix effects, will also affect MIP based assays [22]. [Pg.130]

There may be occasions when a standard is set at a concentration below current analytical limits of detection (LODs) or limits of quantification (LOQs). This could be because high uncertainty leads to the application of large assessment factors to toxicity data to derive a standard or because analytical techniques for a particular environmental matrix have higher LODs/LOQs than those available for the medium in which the standard was derived (e.g., sewage effluent versus laboratory water). An inability to measure concentrations of a chemical at the standard does not necessarily render the standard totally useless. For example, a water quality standard set in a receiving watercourse may be below the LOD/LOQ, but measurement of concentrations from an effluent may be above these limits. Appropriate modeling may allow good estimation of whether the standard in the watercourse has been exceeded. [Pg.44]

Since immunoassays are primarily analytical techniques, in addition to studies for a better understanding of the nature of antibody-antigen interaction, there are continuous efforts to improve immunoassay performance (e.g., sensitivity, selectivity, precision and accuracy) in terms of robustness and reliability when analysing complex samples. The present chapter attempts to summarize the most commonly used immunoassay concepts, as well as the main approaches employed for the improvement of immunoassay sensitivity, selectivity and precision. The discussion is focussed aroimd the main thermodynamic and kinetic principles governing the antibody-antigen interaction, and the effect of diverse factors, such as assay design, concentration of reactants, incubation time, temperature and sample matrix, is reviewed in relation to these principles. Finally, particular aspects on inummoassay standardization are discussed as well as the main benefits and limitations on screening vs. quantification of analytes in real samples. [Pg.578]

Identification of constituents in plastics depends on a number of factors, i.e. the solubility or insolubility of the constituent in the plastic matrix, the fact that many are incorporated at relatively low concentrations, their reactivity and stability. The chemical identity and analysis for constituents may use both chemical and physico-chemical analytical procedures. These range from estimations on density, melt flow index (MFI), ash, melting point, observation of burning, visual characterisation to more sophisticated analytical techniques such as ... [Pg.212]

To ensure the reliability of analytical techniques, they need to be validated. Validation provides information on the overall performance of the assay as well as on individual parameters and factors that can be used to estimate the degree of uncertainty associated with an assay (Ellison et al., 2000). An adequate validation procedure assesses, and therefore ensures, that the immunoassay performs within an acceptable range of established criteria. Parameters used to evaluate the performance of the assays may be affected by (1) factors inherent to the analytical technique, such as antibody specificity and antibody cross-reactivity, and (2) external factors such as environmental conditions (temperature) and type of sample (matrix, processed food vs. raw ingredients). A... [Pg.237]

High-performance liquid chromatography-mass spectrometry (HPLC-MS) is a powerful analytical technique widely used in recent years for the analysis of biomarkers and metabolites. Biomarker determination and quantification, whether metabolic or adducted biomolecules, are commonly used to evaluate exposure and support biomonitoring research, especially in the area of occupational exposure and health. Some of the common problems and strategies of HPLC-MS biomarker analysis involve matrix effects, the use of isotope-labeled internal standard compounds, and sample cleanup usually all of these factors must be evaluated within the development phase of an analysis procedure. Specific examples of biomarker analysis using HPLC-MS include acrylamide, aromatic compounds, and 1-bromopropane, and these examples are discussed in detail. [Pg.238]

Most of the analytical problems arise from three factors the situation of the additive in a more or less insoluble polymer matrix, the high reactivity and low stability of many types of additives, especially antioxidants, and the low concentrations of additives present in many instances in the polymer matrix. The first factor severely limits the choice of analytical techniques that can be applied to the sample without prior separation of the additive from polymer, a procedure which is itself hindered by the nature of the polymer matrix. In addition, any extract of the polymer is liable to contamination by low molecular weight polymer wax which may interfere with subsequent analysis and is difficult to remove. [Pg.3]

Quantitative analysis using FAB is not straightforward, as with all ionisation techniques that use a direct insertion probe. While the goal of the exercise is to determine the bulk concentration of the analyte in the FAB matrix, FAB is instead measuring the concentration of the analyte in the surface of the matrix. The analyte surface concentration is not only a function of bulk analyte concentration, but is also affected by such factors as temperature, pressure, ionic strength, pH, FAB matrix, and sample matrix. With FAB and FTB/LSIMS the sample signal often dies away when the matrix, rather than the sample, is consumed therefore, one cannot be sure that the ion signal obtained represents the entire sample. External standard FAB quantitation methods are of questionable accuracy, and even simple internal standard methods can be trusted only where the analyte is found in a well-controlled sample matrix or is separated from its sample matrix prior to FAB analysis. Therefore, labelled internal standards and isotope dilution methods have become the norm for FAB quantitation. [Pg.369]

Selection of a suitable ionisation method is important in the success of mixture analysis by MS/MS, as clearly shown by Chen and Her [23]. Ideally, only molecular ions should be produced for each of the compounds in the mixture. For this reason, the softest ionisation technique is often the best choice in the analysis of mixtures with MS/MS. In addition to softness , selectivity is an important factor in the selection of the ionisation technique. In polymer/additive analysis it is better to choose an ionisation technique which responds preferentially to the analytes over the matrix, because the polymer extract often consists of additives as well as a low-MW polymer matrix (oligomers). Few other reports deal with direct tandem MS analysis of extracts of polymer samples [229,231,232], DCI-MS/MS (B/E linked scan with CID) was used for direct analysis of polymer extracts and solids [69]. In comparison with FAB-MS, much less fragmentation was observed with DCI using NH3 as a reagent gas. The softness and lack of matrix effect make ammonia DCI a better ionisation technique than FAB for the analysis of additives directly from the extracts. Most likely due to higher collision energy, product ion mass spectra acquired with a double-focusing mass spectrometer provided more structural information than the spectra obtained with a triple quadrupole mass spectrometer. [Pg.403]


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