Ethylenediamine tetraacetic disodium

Para-phenylenediamine is an excellent reagent for detection of an aldehyde group in a hchen substance. Its disadvantages are instability and suspected carcinogenic activity. To overcome the rapid oxidation of an alcoholic solution of p-phenylenediamine a method for stabilizing the solution for at least one year by adding disodium edetate (ethylenediamine tetraacetic disodium salt) has been described 441). 

Ethylenediamine tetraacetic acid (EDTA) [60-00-4] (Sequestrene), an anticoagulent at 1 mg of the disodium salt per mL blood, complexes with and removes calcium, Ca ", from the blood. Oxalate, citrate, and fluoride ions form insoluble salts with Ca " and chelate calcium from the blood. Salts containing these anticoagulants include lithium oxalate [553-91-3] 1 mg/mL blood sodium oxalate [62-76-0]2 mg/mL blood ... 

The perturbation of monolayers with agents (e.g., disodium ethylenediamine tetraacetate, Ca+2-free medium, sodium citrate, cytochalasin D) to open tight junctions and the effect on the transmonolayer flux of permeants are addressed in this section. It has been observed that permeants taking predominantly the trans-cellular route are not affected by perturbants of the paracellular route, compared to extracellular or relatively hydrophilic permeants (Artursson and Magnusson, 1990). Let us put these general observations into a quantitative intepretation in the light of the transmonolayer kinetic studies of steroids in this section and of paracellular permeants in Section III. There are three cases to consider (1) ABL-controlled permeants, (2) monolayer-controlled permeants transported principally by the transcellular route, and (3) monolayer-controlled permeants for which the paracellular route dominates. 

Cory-Slechta DA, Weiss B, Cox C. 1987. Mobilization and redistribution of lead over the course of calcium disodium ethylenediamine tetraacetate chelation therapy. J Pharmacol Exp Ther 243 804-813. 

Aqueous solution of ethylenediamine tetraacetic acid disodium salt (Na2 (EDTA)), 4 x 10 4M, 20 mL... 

Charge item 1, sucrose, D-mannitol, saccharin sodium, and disodium ethylenediamine tetraacetate in an agitating granulator. 

Tris(hydroxymethyl)methylamine (TRIS), sodium chloride, sodium citrate, ethylenediamine tetraacetic acid disodium salt (EDTA), lithium chloride, Tween 20, streptavidin 10 nm colloidal gold labelled, hydrochloric acid (37%), nitric acid, streptavidin-coated paramagnetic beads (MB) with a diameter of 2.8 pm, Dynabeads M-280 Streptavidin (Dynal Biotech, Oslo, Norway) biotinylated probe oligonucleotides which sequences are shown in Table 53.1. 

EDTA reagent Add 50 g disodium ethylenediamine tetraacetate dihydrate to 100 mL of 10% NaOH solution (10 g NaOH/100 mL distilled water). Heat if necessary to dissolve. 

Simultaneous determination of chromium(lll) and chromium(VI) in water extract from metal fumes Sample solution at pH 5 reacted with disodium ethylenediamine tetraacetic acid at 50 °C for 1 hour HPLC on anion exchange column with Na2C03 eluting solution and simultaneous UV and AAS detection 0.2 ng by UVfor chromium(VI) 2.0 ng by UV 5.0 ng by AAS for chromium (IV) 5 ng by AAS for chromium (III) 95-105% at 0.002-2.0 pg Suzuki and Serita 1985... 

These reactions were reported to give inconsistent results1. Purification of the phenyliodo-niobenzoates by rccrystallization from water in the presence of disodium ethylenediamine-tetraacetate gave the monohydrates that reacted reproducibly with the ditellurium compounds2. 

Barium sulphate precipitate may also be dissolved in a hot 3 to 5 per cent solution of disodium ethylenediamine tetraacetate (Na2EDTA) in the presence of ammonia. 

Calcium chloride solutions (pH =6.2) labeled with Ca or 36ci were displaced vertically downward through columns of homogeneous, repacked, water-saturated sandy soil by a chemically identical solution labeled with Cl or Ca, respectively. Constant water fluxes, and solution activities of 1 to 15 pCi/dm, were used. Calcium solutions were analyzed by titration with disodium dihydrogen ethylenediamine tetraacetate to a murexide end point (11). The activity of radioactively labeled solutions was obtained by liquid scintillation techniques. Concentrations of adsorbed calcium were calculated from isotope dilution. The concentration of calcium chloride in the influent solution was 0.08 N. Because exchange of calcium for itself was the only chemical process affecting transport, the calcium chloride concentration remained constant at 0.08 N throughout each experiment, both within the column and in the effluent. 

As a means of decreasing side reactions in the dehydrogenation of tertiary amines with mercuric acetate, Knabe studied the oxidation of alkaloids of the type of papaverine (1) in the presence of an equivalent amount of the sequestering agent ethylenediamine tetraacetic acid (EDTA) as the disodium salt. The products were the papaverinium salt (2) and the dihydro salt (3) in the yields noted- In the absence of the complexing agent, papaverine is cleaved at the methylene bridge. 

EDTA solution approx. 0.01 (N) Dissolve 2.0 g disodium dihydrogen ethylenediamine tetraacetate (Na2 H2 CjoH 2 8 2 2H2O) and 0.05 g magnesium chloride hexahydrate in one litre water and standardize against standard calcium solution. 

Leonard and coworkers " have developed the mercuric acetate oxidation of cyclic tertiary amines into a eneral method for the synthesis of heterocyclic enamines. This method has been used by other authors , A solution of the amine with a four-molar excess of mercuric acetate in 95% aqueous acetic add was refluxed on a steam bath and, after 1.5 hours, the mercurous acetate had precipitated and the amine obtained in 60-80% yield. It was assumed that 1,2-elimination, which requires an antiperiplanar arrangement of the nitrogen-electron pair and the eliminated hydrogen atom, took place, and that elimination of the hydrogen atom on the tertiary carbon atom is preferred, Oyeroxidation can be avoided by adding disodium ethylenediamine-tetraacetate to the reaction mixture . 

Problems (and solutions) include inaccurate and/ or unstable pH readings caused by crosscontamination (rinse electrode assembly with distilled water and blot dry between measure-ments) development of a protein film on the surface of the electrode (soak in 1% w/v pepsin in 0.1 mol L-1 HCI for at least an hour) deposition of organic or inorganic contaminants on the glass bulb (use an organic solvent, such as acetone, or a solution of 0.1 mol L 1 disodium ethylenediamine-tetraacetic acid, respectively) drying out of the internal reference solutions (drain, flush and refill with fresh solution, then allow to equilibrate in 0.1 mol L 1 HCI for at least an hour) cracks or chips to the surface of the glass bulb (use a replacement electrode). 

Sodium-ethylenediamine tetraacetic acid (EDTA) Disodium hydrogen phosphate 0.35 mg 13.3 mg Ascorbic acid 12 mg... 

Ammonium chloride Ammonium sulfate Barium chloride Calcium chloride Cesium chloride Citric acid Copper sulfate Disodium ethylenediamine tetraacetate (EDTA sodium) Ethanol Ethylene glycol Ferric chloride Formic acid D-Fructose D-Glucose Glycerol... 

The cysteinato complex A (1.2 g, 0.0019 mol) and disodium ethylenediamine-tetraacetate dihydrate Na2H2edta-2H20 (0.80 g, 0.0021 mol) are dissolved in hot water (18 mL), to which concentrated HCI (2.5 mL) is added. The temperature of the solution is kept above 90°C for 5 min. After the solution is ice-cooled, the pH of the solution is adjusted to 6 by use of concentrated NaOH. Then, MgCl2 (0.8 g) dissolved in water (2 mL) is added and the solution is kept in a refrigerator overnight. The crystals that deposited are filtered and washed with methanol and diethyl ether successively and air-dried. Yield 1.0 g (75%). 

Synonyms cas 139-33-3 ethylenediamine tetraacetic acid disodium salt disodium tetracemate ... 

Toxic and sublethal effects of lead and its compounds on birds held under controlled conditions vary widely with species, with age and sex, and with form and dose of administered lead. Several generalizations are possible decreased blood ALAD and increased protoporphyrin IX activity levels are useful early indicators of lead exposure lead shot and certain organolead compounds are the most toxic forms of lead nestlings are more sensitive than older stages and tissue lead concentrations and pathology both increase in birds given multiple doses over extended periods. Blood lead concentrations of lead-poisoned waterfowl may be reduced with initiation of disodium calcium ethylenediamine tetraacetate therapy however, ALAD levels remained depressed. 

Synonyms Disodium dihydrogen ethylenediaminetetraacetate Disodium edetate Disodium ethylenediamine tetraacetate Edetate disodium Ethylenediaminetetraacetic acid, disodium salt (Ethylenedinitrilo) tetraacetic acid, sodium salt Glycine, N,N -1,2-ethanediylbis[N-(carboxymethyl)-, disodium salt dihydrate Classification Substituted diamine Empirical CioHi4N2Na20s Formula rOOCCH2)2NCH2CH2N(CH2COO-)2 2H 2Na ... 

Disodium ethylenediamine tetraacetate. See Disodium EDTA Disodium etidronate CAS 7414-83-7... 

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