Biotinylated oligonucleotide probes

Lebaron, P. Catala, P. Fajon, C. Joux, F. Baudart, J. Bernard, L. A new sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification. Appl. Environ. Microbiol. 1997, 63, 3274-3278. 

Cubie, H.A., and Norval, M. (1989) Detection of human papilloma viruses in paraffin wax sections with biotinylated synthetic oligonucleotide probes and immunogold staining./. Clin. Pathol. 42, 988-991. 

Lloyd, R.V., Jin, L., and Fields, K. (1990) Detection of chromogranins A and B in endocrine tissues with radioactive and biotinylated oligonucleotide probes. Am. I. Surg. Pathol. 14, 35-43. 

Perhaps the most common method of DNA biotinylation is through enzymatic incorporation with the use of a biotin-labeled deoxynucleoside triphosphate. First reported by Langer et al. and Leary et al. in 1981, the procedure is probably the most popular nonradioactive labeling technique reported for oligonucleotide probes. Although biotinylated derivatives of dCTP and dATP are reported in the literature, by far the most frequently employed derivative is biotin—dUTP prepared from the reaction of an amine-modified dUTP with an amine-reactive biotinylation reagent, such as NHS-LC—biotin (Chapter 8, Section 3.1). 

Breast cancer E908X-WT DNA Hybridisation of biotinylated target strand to oligonucleotide probe-coated magnetic beads, then addition of streptavidin-Au particles Dissolution of Au tag, and its chronopotentiometric stripping analysis at a single-use SPCE +5.0gA... 

Immobilisation of oligonucleotide probes onto the electrode surface 40 pL of 3 -biotinylated oligonucleotide probes (0.5ng/pL) is left on the electrode surface for 15 min. Finally, the electrodes are rinsed with 2 x SSC buffer pH 7.2 containing 1% of BSA. 

Form the sensing phase placing 40 pL of S -biotinylated oligonucleotide probes (0.5ng/pL) for 15 min. 

The LiPA HIV-1 RT typing test is based on the reverse hybridization principle. Biotinylated DNA material obtained during PCR is hybridized with specific oligonucleotide probes immobilized as parallel lines on membrane-based strips. After hybridization, streptavidin labeled with alkaline phosphatase is added and bound to any biotinylated hybrid previously formed. Incubation with BCIP/NBT chromogen results in a purple/brown precipitate. 

Pringle JH, Ruprai AK, Primrose L, et al. In situ hybridization of immunoglobulin light chain mRNA in paraffin sections using biotinylated or hapten-labelled oligonucleotide probes. J Pathol. 1990 162(3) 197-207. 

Fig. 43. General scheme for sandwich-type gene probe assays utilizing dioxetanes of the type shown in Fig. 41. Both alkaline phosphatase (Aik. Phos.) and biotin (B) are covalently attached to oligonucleotide probes. In the scheme shown on the right-hand side, the enzyme is covalently attached to streptavidin (SA), which is in turn captured by hybridizing to a biotinylated probe.

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