Nickel disodium EDTA

Nickel salts administered by intravenous or subcutaneous injection are comparatively toxic. For all routes of parenteral administration, the LD50 (lethal dose to 50% of the sample) range for injected nickel salts is 6.0 mg Ni/kg BW for dogs given nickel oxide intravenously to 600.0 mg Ni/kg BW for mice given nickel disodium EDTA intraperitoneally. 

Mix 0.01 M dimethyltryptamine, 0.02 M phosphate buffer pH 7.2 containing 5 mM ascorbic acid, 0.02 M disodium EDTA and 0.01 M ferrous sulfate (CuCI may substitute) and add with stirring at 20-22° 0.02 M H202 (0.01 M may increase yield). Let reaction proceed to completion (2 hours or less) and extract with ethyl acetate. Dry and evaporate in vacuum to get about 30% yield of psilocin. The product, which contains the other OH-DMT s as well, can be chromatographed on silica thin layer with t-butanol-acetic acid-water (ACS 22,1210 (1968)) or on a 5% alumina-Nickel... 

Note A number of salts of EDTA are available with uses identical or similar to the acid. The USP salts are called edetates (calcium disodium, disodium edetates) others are usually abbreviated to EDTA (tetrasodium, trisodium EDTA). Other salts, known chiefly under trademark names, are the sodium ferric, dihydrogen ferrous and arange of disodium salts with magnesium, divalent cobalt, manganese, copper, zinc, and nickel. 

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