Essential EDTA

EDTA Efhylenediamineletra-acetic acid. EFA Essential fatty acids. ... 

Calcium is essential to several steps in the enzyme cascade of the blood clotting process, such as the conversion of prothrombin to thrombin (23). Clotting can be inhibited in stored blood suppHes by addition of complexing agents such as EDTA or citrate which reduce the levels of the free ion, Ca(Il). 

Relationship between Ca2+ concentration and luminescence intensity. In the measurement of Ca2+ concentration with aequorin, the calibration of the relationship between Ca2+ concentration and luminescence intensity is essential. However, the application of this relationship is complicated by the chelator used to set the Ca2+ concentration, for the reason noted above. To minimize the complication, we used only a minimum amount of EDTA to protect aequorin in the measurements to obtain the relationship between Ca2+ -concentration and light intensity, and plotted the data as shown in Fig. 4.1.7 (Shimomura and Johnson, 1976). The concentration of EDTA was... 

EDTA and NTA are essentially the only two chelants formulated into BW deposit control programs. The rationale for selection of one product over another is often a point of contention. Looking first at the relative chelation stability constants (chelate log values, Ks) ... 

The rates are essentially independent of the distribution of metal in the MT with similar rates between Zn7—MTm (Cd, Zn)7-MT, and Cd7—MT. The values of the rate constants are ks = 6.9( 0.9) x 10 " s and kf=2.7( 1.2) x 10 s for the holo-protein. The slow rate constant is similar in magnitude to the first-order protein-dependent steps observed for reactions of DTNB (5,5 -dithiobis(2-nitrobenzoate)), EDTA (ethylenediamine tetraacetate), cisplatin, and other reagents, which has been attributed to a rearrangement of the protein. The fast step is more rapid by an order of magnitude, which suggests that other mechanisms are prevailing. 

EDTA Ethylenediamine tetraacetic acid ako known as etidronic acid EE Eosinophilic eosinophils EEG Electroencephalogram EET Epoxyeicosatrienoic acid EFA Essential fatty acid EFS Electrical field stimulation EGl Monoclonal antibody specific for the cleaved form of eosinophil cationic peptide... 

Excessive lead exposure has also been implicated as a causative agent in kidney disease associated with gout and essential hypertension (Batuman et al. 1981, 1983). Gout patients with renal impairment, and hypertensive patients with renal impairment, had significantly higher lead stores (as determined by the 3-day EDTA lead mobilization test) than gout patients or hypertensive patients without renal impairment, respectively. Therefore, excessive lead absorption may be involved in the renal impairment seen in patients with gout or essential hypertension. 

Water soluble Se in the North West region varied from 0.0002-0.0429 mg/kg. Water soluble Se accounted for 2.13-6.34% of the total Se in the soils of North China. Selenium is an essential element to animals and humans. When water soluble Se in soils is less than 0.003 mg/kg, Se deficiency in animal and human beings may occur. EDTA-extractable Se in the alkali desert soils of North China was in the range of 0.011-0.090 mg/kg this was about 5-11% of the total Se in the soils. Selenium deficiency was mostly found in the Loess Plateau and Tibet region. NH4OAc-cxtractable Ni in soils from Beijing was 0.29 mg/kg. 

The poly(3HO) depolymerase differs from poly(HASCL) depolymerases in several of its biochemical properties it is specific for poly(HAMCL) and for artificial esters such as p-nitrophenylacyl esters with six or more carbon atoms in the fatty acid moiety. Poly(3HB) and other poly(HASCL) are not hydrolyzed. The enzyme is not inhibited by dithioerythritol or by EDTA and therefore apparently does not contain essential disulfide bonds. It is also not dependent on Ca2+ or other divalent cations. 

It is possible to determine the concentration of certain metal ions by performing a titration in which the complexation of the metal is the essential reaction. Typically, a chelating agent such as EDTA is used because the complexes formed are so stable. The specific composition of complexes formed in solutions often depends on the concentrations of the reactants. As a part of the study of the chemistry of coordination compounds, some attention must be given to the systematic treatment of topics related to the composition and stability of complexes in solution. This chapter is devoted to these topics. 

In general, all EDTA complexation reactions essentially have the ratio of EDTA to metal ion as 1 1. Thus, we have ... 

Since hematin inhibits Taq polymerase, it is absolutely essential to eliminate red blood cell contamination. Selective lysis of red blood cells can be accomplished with a buffer mixture consisting of 155 mM ammonium chloride, 10 mM potassium bicarbonate, and 0.1 mM EDTA adjusted to pH 7.4. Alternatively, the cytoplasmic membrane of all cells can be dissolved with a buffer mixture containing the non-ionic detergent Triton-X 100, leaving behind nuclei of white blood cells from which DNA can be extracted. However, this technique will result in the loss of cytoplasmic DNA to the supernatant, and hence will not be able to extract mitochondrial DNA (B11). 

In the literature there are only few studies on the water-exchange processes of the manganese(II) species in general (33,38- 1), and the only seven-coordinate Mn(II) complexes studied are [Mn(EDTA) (H20)] and its derivatives (38,39,42,43). Such studies are essential for understanding the mechanism of the manganese-containing SOD mimetics. The volume of activation for the water-exchange reaction... 

Methods based on the inhibitory effect of the analyte and the use of an enzyme thermistor have primarily been applied to environmental samples and typically involve measuring the inhibitory effect of a pollutant on an enzyme or on the metabolism of appropriate cells [162]. The inhibiting effect of urease was used to develop methods for the determination of heavy metals such as Hg(II), Cu(II) and Ag(I) by use of the enzyme immobilized on CPG. For this purpose, the response obtained for a 0.5-mL standard pulse of urea in phosphate buffer at a flow-rate of 1 mL/min was recorded, after which 0.5 mL of sample was injected. A new 0.5-mL pulse of urea was injected 30 s after the sample pulse (accurate timing was essential) and the response compared with that of the non-inhibited peak. After a sample was run, the initial response could be restored by washing the column with 0.1-0.3 M Nal plus 50 mM EDTA for 3 min. Under these conditions, 50% inhibition (half the initial response) was obtained for a 0.5-mL pulse of 0.04-0.05 mM Hg(II) or Ag(I), or 0.3 mM Cu(II). In some cases, the enzyme was inhibited irreversibly. In this situation, a reversible enzyme immobilization technique... 

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