Detergent ionic

A -Nonanoyl-n-methylglucamine (Mega-9) [85261-19-4] M 335.4, m 87-89 . A non-ionic detergent purified as n-decanoyl-A -methylglucamine above. [Hildreth Biochem J 207 363 1982.]... 

Reverse transcriptase (from avian or murine RNA tumour viruses) [9068-38-6] [EC 2.7.7.49]. Purified by solubilising the virus with non-ionic detergent. Lysed virions were adsorbed on DEAE-cellulose or DEAE-Sephadex columns and the enzyme eluted with a salt gradient, then chromatographed on a phosphocellulose column and enzyme activity eluted in a salt gradient. Purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 7977 Smith Methods Enzymol 65 560 1980 see commercial catalogues for other transcriptases.]... 

If the unit becomes badly fouled with suspended matter (for example, after a pipe brake has introduced excessive suspended matter into the system) it must be taken out of service and cleaned. This is done with an extended backwash, possibly at higher flow rates. If this does not remove the dirt, the manhole should be opened and the resin agitated with an air lance. Non-ionic detergents can be used, but not at the same time as the air lance or the resulting froth will be impossible to control. 

We described here the characterisation of the pemB gene and its product the second PME of E. chrysanthemi. The biochemical analysis of the purified protein indicated that PemB is actually an enzyme that demethylates pectin, leading to formation of methanol and PGA. However, PemB is more active on methylated oligogalacturonides than on polymeric pectin. The activating effect of non-ionic detergents on PemB was never pointed out for other pectinases and it is a characteristic of many membrane enzymes (21). 

The membrane-bound preparation from kidney is easily solubilized in non-ionic detergent and analytical ultracentrifugation shows that the preparation consists predominantly (80 85%) of soluble af units with 143000 [28]. The soluble a)S unit maintains full Na,K-ATPase activity, and can undergo the cation or nucleotide induced conformational transitions that are observed in the membrane-bound preparation. A cavity for occlusion of 2K or 3Na ions can be demonstrated within the structure of the soluble a)S unit [29], as an indication that the cation pathway is organized in a pore through the aji unit rather than in the interphase between subunits in an oligomer. 

Detergents have been used for simple solubilization of SWNTs in aqueous solution. Ionic detergents such as SDS will coat the nanotube surface and expose the negatively charged sulfonate groups to the surrounding aqueous environment, thus allowing SWNT dispersion in aqueous... 

Courtot-Coupez and Le Bihan [209,210] determined non-ionic detergents in sea- and fresh-water samples at concentrations down to 2 pg/1 ppm by benzene extraction of the tetrathiocyanatocobaltate (II) (NH4)2 (Co(SCN)4) [ 182] detergent ion-pair, followed by atomic absorption spectrophotometric determination of cobalt [209]. 

Crisp et al. [212] has described a method for the determination of non-ionic detergent concentrations between 0.05 and 2 mg/1 in fresh, estuarine, and seawater based on solvent extraction of the detergent-potassium tetrathiocyana-tozincate (II) complex followed by determination of extracted zinc by atomic AAS. A method is described for the determination of non-ionic surfactants in the concentration range 0.05-2 mg/1. Surfactant molecules are extracted into 1,2-dichlorobenzene as a neutral adduct with potassium tetrathiocyanatozin-cate (II), and the determination is completed by AAS. With a 150 ml water sample the limit of detection is 0.03 mg/1 (as Triton X-100). The method is relatively free from interference by anionic surfactants the presence of up to 5 mg/1 of anionic surfactant introduces an error of no more than 0.07 mg/1 (as Triton X-100) in the apparent non-ionic surfactant concentration. The performance of this method in the presence of anionic surfactants is of special importance, since most natural samples which contain non-ionic surfactants also contain anionic surfactants. Soaps, such as sodium stearate, do not interfere with the recovery of Triton X-100 (1 mg/1) when present at the same concentration (i.e., mg/1). Cationic surfactants, however, form extractable nonassociation compounds with the tetrathiocyanatozincate ion and interfere with the method. 

A very large group of common acceptors are linear, branched or aromatic alcohols. The glycosides formed are widely applicable as, e.g., non-ionic detergents. The alcohol is mostly used as an organic cosolvent and the water... 

Since hematin inhibits Taq polymerase, it is absolutely essential to eliminate red blood cell contamination. Selective lysis of red blood cells can be accomplished with a buffer mixture consisting of 155 mM ammonium chloride, 10 mM potassium bicarbonate, and 0.1 mM EDTA adjusted to pH 7.4. Alternatively, the cytoplasmic membrane of all cells can be dissolved with a buffer mixture containing the non-ionic detergent Triton-X 100, leaving behind nuclei of white blood cells from which DNA can be extracted. However, this technique will result in the loss of cytoplasmic DNA to the supernatant, and hence will not be able to extract mitochondrial DNA (B11). 

Other miscellaneous derivatives of phenol include non-ionic detergents, aspirin, disinfectants (pentachloro phenol), adipic acid (a Nylon 66 intermediate), and plasticizers. 

S3Tithetic detergents are mainly classified into three categories (i) Anionic detergents (ii) Cationic detergents and (iii) Non-ionic detergents... 

Non-ionic Detergents Non-ionic detergents do not contain any ion in their constitution. One such detergent is formed when stearic acid reacts with polyethyleneglycol. 

Rubredoxin is an electron-transfer protein with an Fe(IlI)/Fe(lI) redox couple at -0.31 V (SCE) in water (20). Our peptide model, [Fe( Cys-Pro-Leu-Cys-OMe)2] (Z = benzyloxycarbonyl) (21) exhibits its Fe(lll)/Fe(ll) redox couple at -0.50 V (SCE) in Mc2SO (9). This is similar to the value observed for the native protein when the difference of the solvent is taken into account. When the model complex is solubilized in water by formation of micelles with addition of the non-ionic detergent, Triton X-KX), we also observed a quasi-reversible redox couple at -0.37 V (SCE) (5). The Fe(lll) complexes of Cys-X-Y-Cys peptides also exhibit a characteristic MCD band at 350 nm due to ligand-to-metal charge transfer which has also been found in oxidized rubredoxin (4). 

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