Growth of Human Diploid Fibroblasts for Vaccine Production Multiplate Culture

In this section growth in plastic tissue culture vessels with scale-up to multiplate units is described. [Pg.254]

PROCEDURE PROPAGATION AND SUBCULTIVATION OF HUMAN DIPLOID CELLS IN ISO-cm PLASTIC CULTURE VESSELS [Pg.254]

This method describes the establishment and subcultivation of 150-cm cell cultures originating from a human diploid working cell bank. The harvests of these cultures will be used to seed further similar vessels or microcarrier culture systems. [Pg.254]

Cell and Tissue Culture Laboratory Procedures in Biotechnology, edited by A. Doyle and J.B. Griffiths. 1998 John Wiley Sons Ltd. [Pg.254]

Place frozen ampoule/vial of MRC-5/WI-38 from a working cell bank (3.0 x 10 cells at a predetermined population doubling level (PDL)) in warm water [Pg.255]

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