Culture media definition

The site of assembly of the Lp(a) particle, by covalent linkage of apo-B100 to apo(a), is not definitively established. White et al. (W12) proved in baboon hepatocytes that inside the cell two types of apo(a) existed, of which only the larger form was recovered from the culture medium. The lower-molecular-weight form proved to be a precursor with a prolonged residence time in the endoplasmatic reticulum. Density gradient ultracentrifugation and immunoblot analysis showed that the majority of apo(a) was secreted into the medium in a... 

In most instances the test system will be self-evident (e.g., the animal to which the test article is administered or applied). Studies with micro-organisms, however, sometimes present difficulty in defining the test system. In the case of the Ames test, for example, the test system is not merely the colonies of salmonella or yeast, but includes in addition the culture medium, metabolic activation agent (if any), biotin, histidine, and buffer (if any). The last sentence of the definition makes it clear that untreated control groups also meet the definition of test system even though a test or control article is not administered or applied to such groups. 

Mycobacterium tuberculosis. Many polysaccharides from this organism have been studied10 and have been isolated from the culture medium, the somatic portion of the cell, and the cell lipoids. Only in a few cases have the structures been definitely established. 

Synonyms Lactis proteinum Protein, whey Whey protein concentrate Definition Polypeptide obtained from the fluid part of milk after separation from curds Uses Cosmetic protein protein source in animal feeds source of lactose and lactic acid synthesis of riboflavin, acetone, butanol, fuel-grade ethanol cheesemaking culture medium in rigid polyurethane foams moisture retention aid, emulsifier, stabilizer, opacifier, protein fortifier, fat replacer in foods Regulatory FDA 21CFR 135.110, 135.140, 184.1979c... 

The specialist trade also supplies dry culture media in powder form, which contain all the components which are necessary for the cultivation of microorganisms on the particular culture medium. In accordance with the recipes which are given, a definite quantity of the powder must be weighed out. A specified quantity of demineralized water, tap water or the water which is to be analyzed is then poured over this powder. This is then generally dissolved with heating and the pH is set to the desired value. After the solution has been put into test tubes or flasks, it is sterilized either in an autoclave or by sterilization in stages in a steam bath. It is always to... 

For definitive testing of the totipotency concept, single cells or protoplasts must be picked axenically from the culture medium and placed on a suitable growing medium then through manipulation of the mineral, vitamin, amino acid, and hormonal components of the medium cause development of a tissue (usually callus) that gives rise to embryoids that in turn develop roots, stems. 

In recent years there has been a drive to remove serum, serum proteins and other animal-derived materials from cell culture media, motivated in large part by concerns regarding the potential introduction of adventitious agents. The removal of complex additions such as proteins offers other advantages particularly cost reduction and easier purification of product. In addition, chemical definition of the medium greatly assists process optimization. 

The determination of MIC of antibiotics was performed by microtiter method. Antimicrobial agents were diluted by serial dilutions MPB in flat-bottomed plates, they were also added to the culture of Staphylococcus aureus, and were incubated for 24 hr at 34°C. The control was culture Staphylococcus aureus without antimicrobial agents. After that mixture sowed on solid nutrient medium Mueller-Hinton agar for calculation of amount of colony forming particles and MIC definitions. The MIC was considered to be the lowest concentration, which retards the growth of Staphylococcus aureus during the incubation period. 

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