Cell seeding

Stock et al. used P4HB scaffolds and tissue engineered the patch with a porosity of 95% and pore sizes in the range of 180-240 p,m by salt-leaching and solvent evaporation. The sheep autologous cells (endothelial, smooth muscle, and fibroblast cells) were seeded on the scaffold before implantation. Results confirmed that the cell-seeded implants induced progressive tissue regeneration with no thrombus formation, stenosis, or dilatation. 

Beumer GJ, van Blitterswijk CA, Bakker D, and Ponec M. A new biodegradable matrix as a part of a cell seeded skin substitute for the treatment of deep skin defects A physico-chemical characterisation. Clin Mater, 1993, 14, 21-27. 

Stock UA, Sakamoto T, Hatsuoka S, Martin DP, Nagashima M, Moran AM, Moses MA, Khalil PN, Schoen FJ, Vacanti JP, and Mayer JE Jr. Patch augmentation of the pulmonary artery with bioabsorbable polymers and autologous cell seeding. J Thorac Cardiovasc Surg, 2000, 20, 1158-1168. 

Schiraldi et al. [64] have developed this kind of material by combining silica particles and pHEMA. pHEMA is a biocompatible hydrogel that has been widely studied in the past decades due to its chemical-physical structure and mechanical properties. It has been widely used in ophthalmic prostheses (contact or intraocular lenses), vascular prostheses, drug delivery systems and soft-tissue replacement [65]. These authors have shown that by incorporating silica nanoparticles, the resulting hybrid material is highly biocompatible and promotes bone cell adhesion and proliferation of bone cells seeded on it.1 ... 

Correa-Duarte MA, Wagner N, Rojas-Chapana J, Morsczeck C, Thie M, Giersig M (2004). Fabrication and biocompatibility of carbon nanotube-based 3D networks as scaffolds for cell seeding and growth. Nano Lett. 4 2233-2236. 

Alice et al studied the turnover kinetics of Listeria OTonocytogenex-secreted p60 protein (a murein hydrolase) by host cell cytosolic proteasomes. J774 cells, seeded in flasks and incubated overnight in culture medium, were infected with log-phase cultures of E. monocytogenes for 30 min, washed, and incubated in culture medium for 3 h, with gentamicin (50 tg/ml) added after the first 30 min to inhibit extracellular bacterial growth. Cells then were washed and placed in methionine-free medium with spectinomycin, gentamicin, the eukaryotic protein synthesis inhibitors [cycloheximide (50 tg/mL) and anisomycin (30 tg/ml),] and 25 dVI calpain inhibitor I. After 30 min, [ S]methionine was added, and the cells were pulse-labeled for periods of 20 to 60 min. Cells... 

Spheroids are generally used 4 days after cell seeding when, depending on the cell line doubling time, spheroids reach an average size of 300-500 pm (11) (see Note 1). 

Wait at least 24 h after transfection to allow for sufficient expression of the resistance gene before adding antibiotics. Include two plates as controls one plate of transfected cells without antibiotic selection as positive control for cell viability and another plate of untransfected cells, seeded at the same density and treated with blasticidin to monitor cell resistance and antibiotic activity. All cells should die in this control under antibiotic selective pressure. 

When collagen-GAG matrices which can significantly delay the onset of wound contraction are seeded with a minimum density of easily separable skin cells (basal cells) from the same animal, and the cell-seeded matrices are then grafted, wound healing is affected in an even more profound way In this case,... 

A variety of researches on bio-conjugate (or bio-mimicking) materials have been carried out during the last few years. As seen in Table 1, the aims and scope of many of the researchers are directly connected with clinical applications. For instance, endothelial-cell seeding (or sodding) on the luminal surface of vascular grafts is a widely-known technique [165-169] for improving the blood compatibility of polymetric materials. On the other hand, not a few of researchers are oriented to the exploitation of future possibilities of biomaterials. 

Adherence to Principles of GMP in a Prechnical Developmental Process Efficient Standardized MSC Propagation Using Low Cell Seeding Density Superior MSC Proliferation Resulting from HPL-Driven as Compared to FBS-Driven Cultures... 

Efficient Standardized MSC Propagation Using Low Cell Seeding Density... 

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