Tissue regeneration cell seeding

In order to perform its function well, after implantation a scaffold must have the best achievable biocompatibility, it should also have a microstructure resembling that of natural tissue (for cell seeding, ingrowth and proliferation), adequate mechanical strength and biodegradability matching the time-scale of tissue regeneration. 

Stock et al. used P4HB scaffolds and tissue engineered the patch with a porosity of 95% and pore sizes in the range of 180-240 p,m by salt-leaching and solvent evaporation. The sheep autologous cells (endothelial, smooth muscle, and fibroblast cells) were seeded on the scaffold before implantation. Results confirmed that the cell-seeded implants induced progressive tissue regeneration with no thrombus formation, stenosis, or dilatation. 

These mature, but naive T cells exit peripheral blood and seed lymphoid organs in T-cell specific zones to be acquired by adaptive immune responses for elimination of infected or tumor cells, support for humoral immune responses, formation of immunologic memory, prevention of excessive tissue damage, and facilitation of tissue regeneration (see Chapter 12). 

In 2010, Jeong et al. published a study to compare the performance of chondrocyte-seeded scaffolds made of similar architectures to determine the influence of material on in vitro cartilage regeneration [140]. Three-dimensional scaffolds of the same design were fabricated using PCL, polyCglycerol sebacate) (PGS), or POC, and tissue regeneration was characterized by cell phenotype, cellular proliferation and differentiation, and matrix production. The... 

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