Seeding of cells

Schematic of Flow Chip Seeding of Cells to Each Chamber Rowing of Culture medium...

Leave for 2 hr at room temperature, invert the coverslips (coated side up), and rinse them three times with sterile PBS. The coverslips are now ready for the immediate seeding of cells or can be stored for later use at room temperature for up to 4 months. 

The seeding of cells should be performed at least 36 hr before the import assay. Place up to 12 coated coverslips, coated side facing up, into a 10-cm tissue dish. Trypsinize cells from a confluent dish and seed at 1 X 10 cells/lO-cm dish for permeabilization with 0.2 U/ml SLO and at 3 X 10 cells/lO-cm dish for permeabili-zation with 2.0 U/ml. Avoid cell clumping because it impairs the permeabilization with SLO. Two hours before the import assay the medium should be changed. 

GIBCO Cat. No. 21103-049) supplemented with B27-supplement (GIBCO Cat. No. 17504-044). These dishes are stored in the incubator at 37° under 5 % CO2, until seeding of cells. 

Because the tissue engineering technology is based essentially on the seeding of cells into three-dimensional matrices, the material properties... 

Fig. 5- Effect of salt molarity during seeding of cell monolayers with viral RNA on induction of plaque formation. Confluent HeLa cell monolayers in 60 mm petri dishes were washed with balanced salt solution, drained thoroughly and seeded with 0.1 ml of viral RNA in a Tris buffered (0.03 M, pH 8.2) solution containing the stated concentrations of KCl...

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