Cell monitoring

Method Abs, chemical reduction, monitored by absorption spectroscopy CD, chemical reduction, monitored by CD spectroscopy CD/OTTLE, electrochemical reduction using an optically transparent thin layer (OTTLE) cell, monitored by CD spectroscopy CV, cyclic voltammetry EPR, chemical reduction, monitored by EPR. 

Raff Among other things, this raises the question of whether cells sense size at all, or sense something else. What is the evidence that an animal cell monitors its size ... 

Daly, P.F., Lyon, R.C., Faustino, P.J. and Cohen, J.S. (1987) Phospholipid-metabolism in cancer-cells monitored by P-31 NMR-spectroscopy. Journal of Biological Chemistry 262, 14875—14878. 

Ramsay, D., Kellett, E., McVey, M., Rees, S., and Milligan, G. (2002) Homo- and het-erooligomeric interactions between G protein-coupled receptors in living cells monitored by two variants of bioluminescene resonance energy transfer. Biochem. J. 365,429 440. 

G. L. Rossi, D. J. Young, S. I. Wasserman, and K. E. Barrett, Calcium mobilization in activated mast cells monitored by flow cytometric analysis. Agents Actions 31, 257-262 (1990). 

Figure 8 The time-dependent behavior of the hydrated electron obtained in the subpicosecond pulse radiolysis of neat water using 2-mm optical path sample cell, monitored at the wavelength of 780 nm.

Munaka, T., Kanai, M., Abe, H., Fujiyama, Y., Sakamoto, T., Mahara, A., Yamay-oshi, A., Nakanishi, H., Shoji, S., Murakami, A., In situ cell monitoring on a microchip using time-resolved fluorescence anisotropy analysis. Micro Total Analysis Systems 2003, Proceedings 7th pTAS Symposium, Squaw Valley, CA, Oct. 5-9, 2003, 283-286. 

Lyon, R.C., Cohen, I.S., Faustino, PJ Megnin, F., Myers, C.E. (1988). Glucose metabolism in drug-sensitive and drug-resistant human breast cancer cells monitored by magnetic resonance spectroscopy. Cancer Res. 48,870-877. 

A microelectrode alone is not enough it has to be used in connection with a fast scan and not only that, but sometimes with an electrocatalyst. This is the case for single-cell monitoring of insulin and a process called exocytosis in which a bunch of insulin molecules, about 360,000 of them, are deposited, eventually to reach the blood stream and to trigger mechanisms that will consume the glucose that has risen beyond a healthy limit. Remarkably, when one considers the cost to the nation of diabetes, all too little is known about it at this molecular level. To craft a cure, here, too, one must look to the microelectrode and its abilities to obtain sufficient knowledge of events at the level of the individual cell. 

HPLC/OMA System. In this study, the HPLC effluent stream was passed through three flow-cells connected in series with different lengths of Whatman micro-capillary (0.007 " I.D.) tubing. The first flow-cell monitored the absorption at 254 nm and was used to key the recording of absorption and fluorescence spectra of the effluent stream passing through the second and third flow-cells, respectively, at peak maxima. 

Q4 The parathyroids produce a peptide hormone, PTH, which controls the level of calcium in the body. A sensor on the surface of the parathyroid cells monitors blood calcium concentration and PTH is secreted in response to a fall in plasma calcium ion concentration. An increase in the level of PTH leads to hypercalcaemia (raised blood calcium) conversely, a reduction in the level of PTH leads to hypocalcaemia. PTH acts on the kidney to reduce reabsorption of phosphate and at the same time to increase reabsorption of calcium. In addition, it promotes the release of calcium and phosphate into the blood by activating osteoclasts, which break down the inorganic matrix of bone. PTH also increases the absorption of calcium by the mucosal cells of the intestine. The latter is a rather slow, indirect action mediated by PTH stimulation of calcitriol secretion by the kidney. 

Gerisch G, Albrecht R, Heizer C et ah Chemoattractant-controlled accumulation of coronin at the leading edge of Dictyostehum cells monitored using a green fluorescent protein-coronin fusion protein. 

Schulte PF. Risk of clozapine-associated agranulocytosis and mandatory white blood cell monitoring. Ann Pharmacother. 2006 40(4) 683-688. 

Clozapine treatment is associated with risk for agranulocytosis. The use of required weekly white blood cell monitoring and a national registry in the United States has led to a decrease in agranulocytosis to less than 1% [78]. 

Alefacept is well tolerated and its safety profile is equivalent to that of placebo. The most common adverse events are mild and include pharyngitis, influenza-like symptoms, chills, dizziness, nausea, headache, injection site pain and inflammation, and nonspecific infection the frequency of these effects is low. Also, the drug produces no increase in the rate of opportunistic infections or malignancies. Weekly visits to a physician s office for drug administration and CD4 T-cell monitoring are required, and not all patients respond to treatment. 

S. p. Arbault, N. Sojic, D. Bmce, C. Amatore, A. Sarasin and M. Vuillaume, Oxidative stress in cancer prone xeroderma pigmentosum fibroblasts. Realtime and single cell monitoring of superoxide and nitric oxide production with microelectrodes. Carcinogenesis an information retrieval publication, 25(4), 509-515 (2004). 

Euchs, B., Schiller, J. and Cross, M.A., Apoptosis-associated changes in the glycerophos-pholipid composition of hematopoietic progenitor cells monitored by NMR spectroscopy and MALDI-TOE mass spectrometry, Chem Phys Lipids, 150 (2007c) 229-238. 

Figure 5. Fuel Cell Monitoring and Control Electronics Circuit Board Mounted on Scooter Drive Unit...

R. Fields, E.J. Rowley, M. Wilson, and C. Zawodzinski, "A Fuel Cell Monitoring and Control System for a Personal Mobility Vehicle". To be presented at the Electrochemical Society Meeting, Salt Lake City, UT (Fall 2002). 

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