Differentiated cell lines

Van Langendonck, N., Bottreau, E., Bailly, S., Tabouret, M., Marly, J., Pardon, P., and Verge, P. (1998). Tissue culture assays using Caco-2 cell line differentiate virulent from non-virulent Listeria monocytogenes strains. /. Appl. Microbiol. 85, 337-346. 

Fernandez-Martinez AB, Collado B, Bajo AM, Sanchez-Chapado M, Prieto JC, Carmena MJ. 2007. Vasoactive intestinal peptide induces cyclooxygenase-2 expression through nuclear factor-kappaB in human prostate cell lines Differential time-dependent responses in cancer progression. Mol Cell Endocrinol 270 8-16. 

The key to successful transfection is careful optimization of reaction conditions for each individual cell type. Cells of a lower passage number typically respond better to transfection and ensure higher efficiency than those with higher passage number. Also, some cell lines differentiate and change their features after many passages. Conversely, it is best to allow cells to settle down into... 

Among the adverse effects of statins, rhabdomyolysis is considered to be a rare but significant one [30]. Global gene expression analysis of two skeletal muscle cell lines (differentiated rat L6 myo-tubes and a human skeletal muscle cell line) treated with statins... 

Fig. 2 Human pluripotent stem cells (NT2 cell line) differentiated in neuronal culture (96DIV). Groups of neurons are linked by the dense network of neurites, positively stained against NF-200 (green) with cell nuclei co-stained by DAPI...

NB2a mouse neuroblastoma cell line differentiated with dBcAMP (Fowler et aL 2001)... 

NB4I43 mouse neuroblastoma and SK.-N-SH-SY5Y human neuroblastoma cell lines differentiated with retinoic acid (Ehrich etal.. J997)... 

Ranaldi, G. Consalvo, R. et al. (2003). "Permeability characteristics of parental and clonal human intestinal Caco-2 cell lines differentiated in serum-supplemented and serum-free media." Toxicol In Vitro, 17(5-6), 761-7. 

SI. Mnsil, L. S., Cunningham, B. A., Edelman, G. M. and Goodenongh, D.. A. (1990). Differential phosphorylation of the gap jnnetion protein conncxin43 in junctional commnnicarlon-compe-tent and -deficient cell lines. J. Cell Biol. 111(5, pr. 1), 2077-2088. 

Most recently, a phase-I-study defined a dose of 13-ris-retinoic acid that was tolerable in patients after myeloablative therapy, and a phase-III-trial showed that postconsolidation therapy with 13-cis-retinoic acid improved EFS for patients with high-risk neuroblastoma [7]. Preclinical studies in neuroblastoma indicate that ATRA or 13-cw-RA can antagonize cytotoxic chemotherapy and radiation, such that use of 13-cis-RA in neuroblastoma is limited to maintenance after completion of cytotoxic chemotherapy and radiation. It is likely that recurrent disease seen during or after 13-cis-RA therapy in neuroblastoma is due to tumor cell resistance to retinoid-mediated differentiation induction. Studies in neuroblastoma cell lines resistant to 13-cw-RA and ATRA have shown that they can be sensitive, and in some cases collaterally hypersensitive, to the cytotoxic retinoid fenretinide. Here, fenretinide induces tumor cell cytotoxicity rather than differentiation, acts independently from RA receptors, and in initial phase-I-trials has been well tolerated. Clinical trials of fenretinide, alone and in combination with ceramide modulators, are in development. 

Embryonal carcinoma cell lines have also been established in culture from transplantable teratocarcinomas. The embryonal carcinoma (EC) stem cells present in the tumors actually differentiated, and give rise to a variety of different types of embiyonic and adult cells. When these EC cells are cultured, they still possess the... 

Included among other differentiating cell lines which have been established in culture, is the human promyelocytic cell line HL-60, which differentiates into more mature myeloid cells upon treatment with retinoic acid and prostaglandin E] (PGEi). Friend erythroleukemia cells differentiate into hemoglobin-producing cells when treated with either dimethyl sulfoxide, or hexamethylene bis-acetamide. 

Sato, G.H. (1980). Differentiated Animal Cell Lines. Alan R. Liss, New York. 

Musil LS et al. 1990. Differential phosphorylation of the gap junction protein connexin43 in junctional communication-competent and -deficient cell lines. J Cell Biol 111 2077-2088. 

Alexaki A, Quiterio SJ, Liu Y, Irish B, RUareski E, Nonnemacher MR, Wigdahl B (2007) PMA-induced differentiation of a bone marrow progenitor cell line activates HIV-1 LTR-dtiven transcription. DNA CeU Biol 26(6) 387-394... 

Whether or not the production of /1-amyloid can be curtailed, it would be desirable to either replace the damaged neurons or encourage the remaining functional ones to ramify further and exhibit more influence. The former, which requires tissue or cell line grafts, is currently not feasible and barely investigated experimentally but there is much interest in the possible use of neurotrophic proteins (neurotrophins) that encourage neuronal growth and differentiation. 

GAMET L, DAViAUD D, DENis-POUXViEL 0, REMESY 0, MURAT J 0 (1992) Effects of shoit-chain fatty acids on growth and differentiation of the human colon-cancer cell line HT29. Int J Cancer. 52 286-9. 

Hematopoiesis is defined as the development and maturation of blood cells and their precursors. In utero, hematopoiesis may occur in the liver, spleen, and bone marrow. However, after birth, it occurs exclusively in the bone marrow. All blood cells are generated from a common hematopoietic precursor, or stem cell. These stem cells are self-renewing and pluripotent and thus are able to commit to any one of the different lines of maturation that give rise to platelet-producing megakaryocytes, lymphoid, erythroid, and myeloid cells. The myeloid cell line produces monocytes, basophils, neutrophils, and eosinophils, whereas the lymphoid stem cell differentiates to form circulating B and T lymphocytes. In contrast to the ordered development of normal cells, the development of leukemia seems to represent an arrest in differentiation at an early phase in the continuum of stem cell to mature cell.1... 

Zweibaum A, M Laburthe, E Grasset, D Louvard. (1991). Use of cultured cell lines in studies of intestinal cell differentiation and function. In M Field, CA Frizzell, eds. Handbook of Physiology, Section 6, The Gastrointestinal System, Vol. IV, Intestinal Absorption and Secretion. Bethesda, MD Am Physiol Society, pp 223-255. 

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