Promyelocytic cells

Included among other differentiating cell lines which have been established in culture, is the human promyelocytic cell line HL-60, which differentiates into more mature myeloid cells upon treatment with retinoic acid and prostaglandin E] (PGEi). Friend erythroleukemia cells differentiate into hemoglobin-producing cells when treated with either dimethyl sulfoxide, or hexamethylene bis-acetamide. 

Tretinoin, also referred to ATRA, which stands for all-trans-retinoic acid, is a retinoic acid that is not cytotoxic but promotes the maturation of early promyelocytic cells and is specific to the t(15 17) cytogenetic marker. The time to peak concentrations is 1 to 2 hours after an oral dose. The elimination half-life is 21 to 51 minutes.32 These maroon-and-gold capsules are dosed at 45 mg/m2 per day divided into two doses. The most significant side effect is the retinoic acid syndrome, which may occur anywhere from the first couple of days of therapy until the end of therapy and consists of symptoms of... 

The enzyme source was HL-60, a human promyelocytic cell line. Cells were washed in phosphate-buffered saline and suspended and sonicated in a low... 

JbUo O, Derocq J-M, Segui M, Le Fur G, Casellas P (1999) Stimulation of peripheral cannabinoid receptor CB2 induces MCP-1 and IL-8 gene expression in human promyelocytic cell line HL60. FEES Lett 448 273-277. 

Derocq JM, JbUo O, Bouaboula M, Segui M, Clere C, Casellas P (2000) Genomic and functional changes induced by the activation of the peripheral cannabinoid receptor CB2 in the promyelocytic cells HL-60. Possible involvement of the CB2 receptor in cell differentiation. J Biol Chem 275 15621-15628... 

The possibility that the CB2 receptor plays a role in the control of immune cell fate is supported by studies on the gene expression profile ofhuman promyelocytic cells, which show that receptor activation up-regulates genes involved in the cell differentiation program (Derocq et al. 2000). These data, together with reports of CB2-induced blockade of neutrophilic differentiation (Alberich Jorda et al. 2003) and changes in CB2 receptor expression during the differentiation and activation ofhuman B cells (Carayon et al. 1998) point to an involvement of the CB2 receptor in the control of immune cell maturation. 

Vitamin A derivative (retinoid) induces differentiation, maturation of immature promyelocytic cells... 

A number of chemically distinct compounds induce differentiation of the HL-60 human promyelocytic cell line as, dimethyl-sulfoxide (DMSO), 1,25-dihydroxy-vitamin Ds, and bezafibrate. During differentiation, promyelocytic HL-60 cells stop growing, become much smaller in size and acquire the ability to form reactive oxygen species (R.O.S.) as shown by the appearance of the phorbol-12-myristate-13-acetate (PMA)-stimulated respiratory burst, detect by the chemiluminescence technique and other methods. ... 

He P, Liu Y, Zhang M, Wang X, Xi J, Wu D, Li J, Cao Y. Interferon-gamma enhances promyelocytic leukemia protein expression in acute promyelocytic cells and cooperates with allotrans-retinoic acid to induce maturation of NB4 and NB4-R1 cells. Exp Ther Med. 2012 3 776-80. 

The involvement of ADPRT in cell differentiation has been confirmed and very much extended by a number of workers. A. Johnstone and G. WilUams and subsequently W. Greer and G. Kaplan have described the blocking of lymphocyte activation by ADPRT inhibitors. Our splendid conference organiser, F. Althaus, has demonstrated that ADPRT activity is required for the expression of some foetal proteins in adult hepatocytes in culture. G. Williams and A. Johnstone have described how ADPRT inhibitors will block the differentiation, but not the proliferation of the parasite Trypanosoma cmzi. We have shown that the differentiation of a human promyelocyte cell line (HL60) can be blocked by ADPRT inhibitors. 

Transient Formation of DNA Strand Breaks During DMSO-Induced Differentiation of Promyelocytic Cell Line, HL-60... 

Fimognari C, Berti F, Niisse M, Cantelli-Forti G, HreliaP (2004) Induction of apoptosis in two human leukemia cell lines as well as differentiation in human promyelocytic cells by cyanidin-3-O-beta-glucopyranoside. Biochem Pharmacol 67 2047... 

Proliferation, 1448,2321, 2404, 3525 Proliferation of smooth muscle cells, 2298 Proliferative effect, 2399,2402 Proline, 50,1571 Proline redox cycle, 1571 Prolonged nerve block, 66 Prolyl oligopeptidase (POP), 1309 Promastigotes, 845, 1266,1290 Promiscuous tmzymes, 1666 Promyelocytic cell lines, 3561 Pronase E, 1085 Pro-oncogene MDM2, 2404 Pro-oxidant respmise, 2370 Propeller, 2777... 

Gonzalez de Quevedo, M., Alcam, F. J., Gonz lez-Reyes, J. A., and Navas, P., 1991, Growth patterns of the HL-60 human promyelocytic cell line stimulated by ascorbate free radical. Cancer J. 4 262-266. 

Ldpez-Lluch, G., Alcam, F. J., Buron, I., Navas, P., and Quesada, J. M., 1994, Effect of ascorbate and calcitriol on HL-60 promyelocytic cell line proliferation and differentiation, Calcif. Tissue Int. 54 457. 

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