Mouse neuroblastoma cell lines

Tissue Culture Assay. Kogure et al. (48) report a novel tissue culture assay for detecting several types of sodium channel blockers. The mouse neuroblastoma cell line ATCC CCL 131 is grown in RPMI 1640 supplemented with 13.5% fetal bovine serum and 100 pg/ml gentamycin, in an atmosphere of 5% C0 95% air at 37 C. Ninety-six well plates are seeded with 1 x 10 cells in 200 pi of medium containing 1 mM ouabain and 0.075 mM veratridine. Veratridine and ouabain cause neuroblastoma cells to round-up and die. In the presence of sodium channel blockers (e.g., TTXs or STXs), the lethal action of veratridine is obviated and cells retain normal morphology and viability. An important feature of this assay is that a positive test for sodium channel blockers results in normal cell viability. Since bacterial extracts can contain cytotoxic components, this assay offers an advantage over tests that use cell death as an endpoint. The minimum detectable level of TTX is approximately 3 nM, or approximately 1/1000 mouse unit. 

Jamaicamides A, B, and G exhibited cytotoxidty to both the H-460 human lung carcinoma and neuro-2a mouse neuroblastoma cell lines. The LG50S were approximately 15 p,M for all three compounds to both cell lines. All three compounds also exhibited sodium charmel-blocking activity at 5 p,M concentration. In the goldfish toxicity assay, a system that has been useful for the detedion of neurotoxic activity in cmde extrads as well as purified compounds, jamaicamide B was the most active (100 % lethality at 5 ppm after 90 min), followed by jamaicamide G (100 % lethality at... 

Novel cyclic depsipeptides, guineamides A-F 120-125, were isolated from a Papua New Guinea collection of the marine cyanobacterium Lyngbya majuscule Guineamides B 121 and C 122 possessed moderate cytotoxicity to mouse neuroblastoma cell line with IC50 values of 15 and 16 pM respectively. 

Fig. 1. Pertussis toxin-mediated ADP ribosylation of membrane G proteins. Isolated cell membranes (50 ng of protein) from N1E 115 cells (mouse neuroblastoma cell line), N2A cells (mouse neuroblastoma cell line), S49-1 eye cells (S49(-) mutated mouse lymphoma cell line deficient in Ga ), 549 wt cells (wild-type mouse lymphoma cell line), RBL (RBL 2H3 rat basophilic leukemia cell line), GH3 cells (GH3 rat hypophyseal tumor cell line), PC-12 (rat pheochromocytoma cell line), HIT-T15 cells (hamster insulinoma cell line), Y-1 cells (mouse adrenal cortex tumor cell line), 108 cc 15 cells (mouse/rat neuroblastoma x glioma hybrid cell line), HL-60 cells (DMSO-differentiated human leukemia cell line), HL-60 (+PT) cells (HL-60 cells pretreated with 25 ng/ml of pertussis toxin for 24 h prior to preparation of membranes), RINm5F cells (rat insulinoma cell line), and C6-2 cells (rat glioma cell line) were subjected to P-ADP-ribosylation as described in section 4.3.3. Samples were precipitated as outlined in section 4.3.5 and subjected to SDS-PAGE with separating gels containing 8% acrylamide (w/v). An autoradiogram of the dried gel is shown. Molecular masses of marker proteins are indicated (kDa). Modified Ga proteins migrate at approximately 40 kDa. Radioactivity running in front of the 30 kDa marker protein comigrates with the dye front...

NB2a mouse neuroblastoma cell line (Harvey and Sharma, 1980)... 

NBR4 is a mouse neuroblastoma cell line which has been shown to have elevated levels of HPRT protein vivo (3) and higher than... 

J. Brennand/ A.C. Chinault/ D.S. Konecki/ D.W. Melton and C.T. Caskey/ Cloned cDNA sequences of the hypoxanthine/guanine phos-phoribosyltransferase gdfne from a mouse neuroblastoma cell line found to have amplified genomic sequences/ Proc. Natl. Acad. 

Tsuji S, Yamashita T, Tanaka M, Nagai Y. Synthetic sialyl compounds as well as natural gangliosides induce neuritogenesis in a mouse neuroblastoma cell line (Neuro2a). J Neurochem. 1988 50(2) 414-423. 

Yamashita, T., Tsuji, S., and Nagai, Y., 1991, Sialyl cholesterol is translocated into cell nuclei and it promotes neurite outgrowth in a mouse neuroblastoma cell line, Glycobiology 1 149-154. 

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