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Animal cells, cryopreservation

Thus upon comparing the economics of the two methods, embryos and sperm, it is very apparent that the freezing of embryos is considerably more expensive due to the need for more resources for example, with a C57BL/6 background, to produce 250 two-cell embryos for cryopreservation by IVF requires >15 females. If strains are never recovered or only recovered once or twice, then the bulk of this expense remains forever frozen. In contrast, cryopreserving sperm has a low initial cost as only few animals (1-3 males) and relatively little labor and materials are required (12). It is upon recovery from sperm by in vitro fertilization (IVF) that animals and labor are used, but then only the required number animals per recovery are used as the IVF process can be scaled to produce the desired number of offspring. [Pg.26]

Hepatocytes - both freshly prepared or cryopreserved - are commercially available e.g. Gen test, IVT, Xenotech or prepared in-house. Isolation of the animal hepatocytes follows after a two step collagenase perfusion of the liver via the vena portae in situ (Seglen 1976) or via several blunt-end cannulae inserted into vessels available on the cut surface of pieces of human liver obtained from resection. Liver cells are gently scraped out into suspension buffer and washed twice to three times by centrifugation to remove cell fragments and non-vital cells. Hepatocytes are used immediately or cryopreserved for further use. [Pg.505]

Use of ex vivo cell substrates is the most recent technique in vaccine cultivation. In the case of measles and mumps vaccines, the cell substrate is chicken embryo cells that have been generated by trypsin enzyme treatment that dissociates the embryonic cells. Similarly, some rabies vaccines use cells derived from fetal rhesus monkey kidneys. Since the 1960s, cell lines have been generated that can be characterized, banked, and cryopreserved. Cryopreserved cells have been adopted to ensure reproducibility and freedom from contaminating viruses and microorganisms and to bypass the ethical problem of extensive use of animal... [Pg.202]


See other pages where Animal cells, cryopreservation is mentioned: [Pg.198]    [Pg.37]    [Pg.99]    [Pg.212]    [Pg.285]    [Pg.542]    [Pg.1429]    [Pg.142]    [Pg.409]    [Pg.511]    [Pg.107]    [Pg.227]    [Pg.556]    [Pg.125]    [Pg.19]    [Pg.123]    [Pg.102]    [Pg.181]    [Pg.394]   


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