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Protein-Mediated Cell Adhesion on Biomaterial Surfaces

Protein-Mediated Cell Adhesion on Biomaterial Surfaces [Pg.143]

Select proteins that mediate adhesion of specific anchorage-dependent cells (such as osteoblasts, fibroblasts, and endothelial cells) on substrate surfaces have been identified (Underwood and Bennett, 1989 Thomas et al., 1997 Ayad et al, 1994). For example, adsorption of fibronectin and vitronectin on tissue-culture polystryene subsequently enhanced osteoblast, fibroblast, and endothelial cell adhesion (Underwood and Bennett, 1989). More importantly, fibronectin and vitronectin adsorption on borosilicate glass, in a competitive environment, maximized fibroblast and osteoblast adhesion, respectively (Thomas et al., 1997). Ayad et al. (1994) reported that enhanced adsorption of laminin on tissue-culture polystyrene promoted subsequent endothelial cell adhesion. These studies provided evidence that adsorption of specific protein(s) can, subsequently, control select cell adhesion on material surfaces. [Pg.143]

Integrins are a family of transmembrane heterodimeric glycoproteins that are receptors for specific epitopes of extracellular matrix proteins and for other cell-surface molecules (Kramer et al, 1993). Integrins exist as a dimer complex composed of an a-subunit (120-180 kD) noncovalently associated with a /1-subunit (90-110 kD) (Hynes, 1992). At least 8 /1-subunits and 14 -units have been identified and are concentrated at loci, called focal adhesion sites, of close proximity between cells and extracellular matrices on substrates (Hynes, 1992). Focal adhesion sites are points of aggregation of, and are physically associated with, intracellular cytoskeletal molecules that control, direct, and modulate cell function in response to extracellular signals (Schwartz, 1992). [Pg.143]

In addition to mediating cell adhesion, it has been demonstrated that integrin expression by osteoblasts determines their phenotypic expression (see the section Osteoblasts The Bone-Forming Cells ). For example, addition of either soluble concentrations of RGD (Moursi et al, 1996) or antibodies of the integrin pair avp() (Moursi et al., 1997) to confluent osteoblast cultures, blocked initiation and formation of mineral nodules these results provided evidence that integrin-fibronectin interactions leading to extracellular matrix development play a crucial role in osteoblast function (Moursi etal., 1996,1997). [Pg.144]

Reports found in the literature suggested that the peptide sequence lysine-arginine-serine-arginine (KRSR) selectively enhanced osteoblast adhesion by possibly binding to heparan sulfate on the membranes of osteoblasts (Dee et al, 1996). Compared to unmodified glass, Dee (1996) demonstrated enhanced osteoblast, fibroblast, and endothelial cell adhesion [Pg.144]


Fig. 8. Schematic representation of protein-mediated cell adhesion on biomaterial surfaces. Biomaterial surface properties (such as hydrophilicity/hydrophobicity, topography, energy, and charge) affect subsequent interactions of adsorbed proteins these interactions include but are not limited to adsorbed protein type, concentration, and conformation. Changes in protein-surface interactions may alter accessibility of adhesive domains (such as the peptide sequence arginine-glycine-aspartic acid) to cells (such as osteoblasts, fibroblasts, or endothelial cells) and thus modulate cellular adhesion. (Adapted and redrawn from Schakenraad, 1996.)... Fig. 8. Schematic representation of protein-mediated cell adhesion on biomaterial surfaces. Biomaterial surface properties (such as hydrophilicity/hydrophobicity, topography, energy, and charge) affect subsequent interactions of adsorbed proteins these interactions include but are not limited to adsorbed protein type, concentration, and conformation. Changes in protein-surface interactions may alter accessibility of adhesive domains (such as the peptide sequence arginine-glycine-aspartic acid) to cells (such as osteoblasts, fibroblasts, or endothelial cells) and thus modulate cellular adhesion. (Adapted and redrawn from Schakenraad, 1996.)...
C. Protein-Mediated Cell Adhesion on Biomaterial Surfaces... [Pg.143]

The topography of a surface is an influential parameter for the performance of a biomaterial, particularly in the case of tissue engineering. Cells attach to a surface via focal adhesion sites that are based on the presence of cellular attachment proteins and factors (Curtis and Wilkinson 1997). Cell behavior will be heavily influenced by the dimensions of a surface upon which attachment sites can be formed and thus dictate the cell shape and migration (Von Recum and Van Kooten 1996). Topographical cues can therefore mediate cell orientation and migration (Clark et al. 1991),... [Pg.25]


See other pages where Protein-Mediated Cell Adhesion on Biomaterial Surfaces is mentioned: [Pg.152]    [Pg.154]    [Pg.152]    [Pg.154]    [Pg.213]    [Pg.215]    [Pg.507]    [Pg.363]    [Pg.370]    [Pg.927]    [Pg.138]    [Pg.296]    [Pg.91]    [Pg.4]    [Pg.29]    [Pg.340]    [Pg.341]    [Pg.342]    [Pg.18]    [Pg.541]    [Pg.79]    [Pg.80]    [Pg.93]    [Pg.175]    [Pg.175]    [Pg.845]    [Pg.846]    [Pg.111]    [Pg.22]    [Pg.346]    [Pg.371]   


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Adhesion, cell surface

Adhesives surface adhesion

Cell adhesion

Cell adhesion proteins

Cell adhesion, protein-mediated

Cell adhesion, protein-mediated biomaterials

Cell adhesive

Cell mediated

Cell surface

Cell surface proteins

Cell-adhesive proteins

On protein

On-cells

Protein adhesion

Surface adhesion

Surface biomaterials

Surface mediation

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