Cell line colon carcinoma caco

OITATE M, NAKAKI R, KOYABU N, TAKANAGA H, MATSUO H, OHTANI H, SAWADA Y (2001) TranSCellular transport of genistein, a soybean-derived isoflavone, across human colon carcinoma cell line (Caco-2). Biopharm Drug Dispos. 22 23-9. 

The Caco-2 cell line was isolated from a human colon carcinoma, and has been characterized as one of the best in vitro models of intestinal epithelium. Indeed, in contrast to other intestinal cell lines, Caco-2 cells are able to constitute a homogenous monolayer and to spontaneously differentiate into polarized cells, highly similar to human mature enterocytes, after approximately 2 weeks of culture. Furthermore, the Caco-2 cells present microvillosities at the apical side and have a high transmembrane resistivity, which confirms the fact that the cells are confluent and link to one another via gap junctions. Finally, they can absorb different compounds, express many enzymes involved in intestinal metabolic pathways (Pinto et al. 1983, Musto et al. 1995, Salvini et al. 2002), and give reproducible in vitro results consistent with results obtained in in vivo studies (Artursson and Karlsson 1991). 

Pinto, M. et al. (1983). Enterocyte-like differentiation an polarizationof the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 47 323-330. 

Borchardt, R. T., Characterization of the human colon carcinoma cell line (Caco-2) as a model for intestinal epithelial permeability, Gastroenterology 1989, 96, 736-749. 

M. D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assman, P., Happen, K., Fogh, J., Zweibaum, A., Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture, Biol. Cell 1983, 47, 323-330. 

Brandsch, M., Miyamoto, Y., Ganapathy, V., Leibach, F. H., Expression and protein C-dependent regulation of peptide/H+ co-transport system in the Caco-2 human colon carcinoma cell line, Biochem. J. 1994, 299, 253-260. 

Grasset, E., Pinto, M., Dussaulx, E., Z WEI BAUM, A., Desjeux, J.-F., Epithelial properties of human colonic carcinoma cell line Caco-2 electrical parameters, Am. J. Physiol. 1984, 247, C260-C267. 

In various human tumour cell lines (lymphoblastic cell line, CCRF CEM gastric carcinoma cell line, St 23132 hepatoma-derived cell line, HepG2 colon-carcinoma cell line, Caco-2) coumarin [IC50 values ranging from 232 to 522 pg/mL] and its major metabolite 7-hydroxycoumarin [IC50 values ranging from 110 to 436 pg/mL] inhibited... 

UGT1A9. UGT1A9 along with UGT1A6 were inducible by 10 (iM tetra-chlorodibenzodioxin (TCDD) in Caco-2 cells, a human-derived colon carcinoma cell line (78). 

Carriere V, Lessufleur T, Barbat A et al. (1994) Expression of cy-tochrom P450 3A in HT-29-MTX cells and CACO-2 clone TC7. FEBS Letter 355 247-250 Chantret I, Barbat A, Dussaulx E et al. (1988) Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells a survey of 20 cell-lines. Cancer Res 48 1936-1942 Cordon-Cardo C, O Brien JP, Boccia J et al. (1990) Expression of the multidrug resistance product (P-glycoprotein) in human normal and tumor tissues. J Histochem Cytochem 38 1277-1287... 

In vitro experiments can sometimes provide valuable insight into what is happening in vivo that is limiting oral bioavailability. The typical experiments, often employed in tandem, to understand bioavailablilty are determinations of compound solubility, membrane permeability, and stability in subcellular fractions. The membrane permeability assays that are most often employed are either a measurement of permeability through an artificial membrane (Parallel artificial membrane permeability assay, PAMPA, is the most common technique) or a cell monolayer (Caco-2, a human colon carcinoma-derived cell line, is the most common cell monolayer). The subcellular fractions most often employed are plasma (for ester-containing compounds) and liver microsomes with the addition of either reduced nicotinamide adenine dinucleotide phosphate (NADPH) or uridine diphosphoglucuronic acid (UDPGA) as cofactor. 

In order to effectively assess drug transport, a cell line must form polarized monolayers of differentiated enterocytes. In addition, the cells must retain the morphological and biochemical characteristics of the human intestine.8,9 Primary cultures of human enterocytes have low viability and do not differentiate or form monolayers.10 Immortalized cell lines have been developed with mixed results.11 The Caco-2 cell fine, derived from a human colon carcinoma, is the cell culture... 

Hidalgo IJ, Raub TJ, and Borchardt RT. Characterization of Human Colon Carcinoma Cell Line (Caco-2) as a Model System for Intestinal Epithelial Permeability. Gastroenterology 1989 96 736-749. 

Rousset M. The human colon carcinoma cell lines ElT-29 and Caco-2 two in vitro models for the study of intestinal differentiation. Biochimie 1986 Sep 68(9) 1035-1040. 

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