Gene expression, differential

All of these characteristics can be under the regulation of the cell and influenced by the cell culture conditions. The age of the cell monolayer in culture can have a profound impact on the quality of the barrier. In monolayers with actively dividing cells, resistance increases with time in culture as tight junctions form (see Fig. 15, Section III.C.4). Resistance reaches a plateau, then decreases as cell viability declines (Section III.C.4). Time in culture may also be a factor in the expression of polarity, which is related to tight junction formation as well as the state of differentiation of the cells (e.g., differential gene expression). 

Zawia NH, Harry GJ. 1996. Developmental exposure to lead interferes with glial and neuronal differential gene expression in the rat cerebellum. Toxicol Appl Pharmacol 138 43-47. 

Rininger JA et al. Differential gene expression technologies for identifying surrogate markers of dmg efficacy and toxicity. DDT 2000 5 560-568. 

Johnson BJ et al. Differential gene expression in response to adjunctive recombinant human interleukin-2 immunotherapy in multidrug-resistant tuberculosis patients. Infect Immunity 1998 66 2426-2433. 

Differential Gene Expression -cell type specific -developmental-stage specific... 

Liang P, Pardee AB. 2003. Analyzing differential gene expression in cancer. Nat Rev 3 869. 

To K. 2000. Identification of differential gene expression by high throughput analysis. Comb Chem High Throughput Screen 3 235. 

In this review we focus on the use of DNA microarrays for the purpose of differential gene expression analysis. Discussions are intended to provide an overview of DNA microarray technology focusing on themes currently believed important to ensuring a successful DNA microarray experiment. Specifically, we address practical issues surrounding the use of DNA microarray technology with emphasis placed on its utility in the investigation of experimental stroke. 

The results obtained from the histone acetylation and phosphorylation studies suggest that cyclo(Trp-Trp) and cyclo(Pro-Trp) induce differential gene expression through different signal transduction pathways. Cyclo(Trp-Trp) induced the highest level of acetylation of histones whereas cyclo(Phe-Pro) induced high levels of phosphorylation of histones." ... 

Monk, M. (1995) Epigenetic programming of differential gene expression in development and evolution. Dev. Genet. 17, 188-197. 

Table 5.2 Lung Squamous Cell Carcinoma Differential Gene Expression... 

Differential gene expression in astrocytes from human normal and glaucomatous optic nerve head analyzed by cDNA microarray, GLIA, 38, 45-64, 2002. 

Tibshirani R, Hastie T (2007) Outlier stuns for differential gene expression analysis. Biostatistics 8 2-8... 

Bachem, C. W. B., van der Hoeven, R. S., de Bruijn, S. M., Vreugdenhil, D., Zabeau, M., and Visser, R. G. F. (1996). Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP Analysis of gene expression during potato tuber development. Plant J. 9, 745-753. 

The EST has continued to be a popular test subject to further optimization, especially through the addition of alternative pathways of differentiation and the addition of molecular markers of effects. Besides cardiac muscle differentiation, other cell types such as neurons (29-31), osteoblasts (32), adipocytes (33), and hepato-cytes (34) have been generated in dedicated differentiation protocols, providing additional opportunities for testing the interference of differentiation pathways with chemical exposures. The addition of transcriptomics approaches to measure differential gene expression, both as influenced by the differentiation process as well as due to chemical exposure, has proven informative and may enhance the predictability of EST assays (35). The extension of this concept to human embryonic stem cell lines is still in its infancy, but has opened a new realm of options that bypasses the need for interspecies extrapolation (36). 

---