CDNA library, preparing

The amino acid sequence of the human erythrocyte glucose transporter was deduced from the nucleotide sequence of a cDNA clone in 1985 [106]. Polyclonal antibodies raised against the protein were used to screen a Xgtl I cDNA library prepared from the human hepatocellular carcinoma cell line HepG2. (Like many other transformed... 

The screening of a cDNA library prepared from the skin of Ph. sauvagei established the amino acid sequence of several dermorphin precursors. The... 

The main disadvantage of RNA-seq results from the costly price of the NGS platforms. Other shortcomings are the lengthy and biased cDNA library preparation procedures and the required computer power to address the algorithmic challenges to align and... 

To this end, cDNA libraries, prepared from cells or tissues known to be rich in certain receptors, were screened by low stringency hybridization, or were used for polymerase chain reaction (PCR) amplification of candidate genes using degenerate primers. Proof of function was obtained after the... 

Originally it was thought that PMN were terminally differentiated and incapable of synthesizing new proteins however, in the last 20 years, it has been revealed that PMN can synthesize and secrete a plethora of proteins. Gene expression in peripheral blood PMN has been the focus of several recent studies (table 4). In a 3 -directed cDNA library prepared from unstimulated human... 

A X.gtll cDNA library prepared from RNA of 4 day-old etiolated pumpkin cotyledons was screened using an affinity purified antibody against pumpkin aconitase [2]. The aconitase cDNA of 3154 bp was obtained by the combination of immunoscreening and plaque hybridization technique. The aconitase cDNA contains an open reading frame of2694 bp capable of encoding a 98 kD protein. 

The molecular cloning of CVF was accomplished by using a A,gtll cDNA library prepared from a poly-A RNA from freshly obtained cobra venom glands (22). The screening of this expression library was performed with a monospecific polyclonal goat anti-CVF antiserum. 

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