CDNA coding

Illarionov, B. A., et al. (1992). Cloning and expression of cDNA coding for the calcium-activated photoprotein obelin from the hydroid polyp Obelia longissima. Dokl. Akad. Nauk SSSR 326 911-913. 

Prasher, D., McCann, R. O., and Cormier, M. J. (1985). Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium-binding protein. Biochem. Biophys. Res. Commun. 126 1259-1268. 

Expression of a tomato cDNA coding for phytoene synthase in Escherichia coli, ph)doene formation in vivo and in vitro, and functional analysis of the varions trimcated gene prodncts , J Biochem (Tokyo), 116, 980-85. 

Bartley, G.E. et al.. Molecular cloning and expression in photosynthetic bacteria of a soybean cDNA coding for phytoene desaturase, an enzyme of the carotenoid biosynthesis pathway, Proc. Natl. Acad. Sci. USA 88, 6532, 1991. 

Gonadotrophins are now also produced by recombinant DNA technology. The genes, or cDNAs coding for gonadotrophins from several species, have been identified and expressed in various... 

Several companies have expressed the cDNA coding for human factor VIII C in a variety of eukaryotic production systems (human VIII C contains 25 potential glycosylation sites). CHO cells and BHK cell lines have been most commonly used, in addition to other cell lines, such as various mouse carcinoma cell lines. The recombinant factor VIII product generally contains only VIII C (i.e. is devoid of vWF). However, both clinical and preclinical studies have shown that administration of this product to patients suffering from haemophilia A is equally as effective as administering blood-derived factor VIII complex. The recombinant VIII C product appears to bind plasma... 

Cerezyme is produced in a CHO cell line harbouring the cDNA coding for human (i-glucoccr-ebrosidase. The purified product is presented as a freeze-dried powder, which also contains mannitol, sodium citrate, citric acid and polysorbate 80 as excipients. It exhibits a shelf life of 2 years when stored at 2-8 °C. 

Figure 24.1 Flow chart of RACE-PCR protocol adopted with slight modifications from the instruction manual of the Marathon cDNA amplification kit. Poly A+ RNA is used for the generation of an adaptor-ligated cDNA library. Fragments containing the 5 - and the 3 -end of the cDNA coding for the carrier are amplified from the uncloned library with adaptor primers and gene-specific primers. Afterwards, a full-length clone is generated from the individual RACE products by subcloning or end-to-end PCR.

Seelenfreund D, Chiong M, Lobos S, Perez LM (1996) A full-length cDNA coding for phenylalanine ammonia-lyase from Citrus limon (Accession No.U43338) (PGR96-026). Plant Physiol 111 348... 

Several companies have expressed the cDNA coding for human factor VIILC in a variety of eukaryotic production systems (human VIILC contains 25 potential glycosylation sites). 

Helariutta, Y. et al.. Cloning of a cDNA coding for dihydroflavonol-4-reductase (DFR) and characterization of dfr expression in corrollas of Gerbera hybrida var. Regina (Compositae). 

Iltzsch, M.H., Bieber, D., Vijayasarathy, S., Webster, P., Zurita, M., Ding, J. and Mansour, T.E. (1992) Cloning and characterization of a cDNA coding for the a-subunit of a stimulatory G protein from Schistosoma mansoni. Journal of Biological Chemistry 267, 14504-14508. 

The entire amino acid sequence of all polypeptide chains of human fibrinogen has been determined by the methods of protein chemistry and later deduced from the nucleotide sequences of the cDNA coding for the polypeptide chains (Doolittle, 1984 Henschen and McDonagh, 1986). There are 610, 461, and 411 amino acids in each of the common forms of the human Act, Wft, and 7 chains, respectively. The amino acid sequences of the three chains are homologous, but important differences exist, giving rise to specific functions for certain molecular domains. 

Wicki R, Schafer BW, Erne P, Heizmann CW. 1996b. Characterization of die human and mouse cDNAs coding for S100A13, a new member of die S100 protein family. Biochem Biophys Res Commun... 

---