Humans, viii

Several companies have expressed the cDNA coding for human factor VIII C in a variety of eukaryotic production systems (human VIII C contains 25 potential glycosylation sites). CHO cells and BHK cell lines have been most commonly used, in addition to other cell lines, such as various mouse carcinoma cell lines. The recombinant factor VIII product generally contains only VIII C (i.e. is devoid of vWF). However, both clinical and preclinical studies have shown that administration of this product to patients suffering from haemophilia A is equally as effective as administering blood-derived factor VIII complex. The recombinant VIII C product appears to bind plasma... 

Two nucleation processes important to many people (including some surface scientists ) occur in the formation of gallstones in human bile and kidney stones in urine. Cholesterol crystallization in bile causes the formation of gallstones. Cryotransmission microscopy (Chapter VIII) studies of human bile reveal vesicles, micelles, and potential early crystallites indicating that the cholesterol crystallization in bile is not cooperative and the true nucleation time may be much shorter than that found by standard clinical analysis by light microscopy [75]. Kidney stones often form from crystals of calcium oxalates in urine. Inhibitors can prevent nucleation and influence the solid phase and intercrystallite interactions [76, 77]. Citrate, for example, is an important physiological inhibitor to the formation of calcium renal stones. Electrokinetic studies (see Section V-6) have shown the effect of various inhibitors on the surface potential and colloidal stability of micrometer-sized dispersions of calcium oxalate crystals formed in synthetic urine [78, 79]. 

Fig. 6. Share of U.S. market occupied by human albumin/plasma protein fraction, (—) Factor VIII concentrate, (-) intravenous immunoglobulin...

Title VIII Supervision and sanctions Tide X Special provisions on medicinal products derived from human blood and plasma Tide XI Supervision and sanctions... 

Human Medicines Directive 2001/83/EC o Title IV Manufacture and imports, o Title VIII Advertising, Title IX Pharmacovigilance, o Title XI Supervision and sanctions... 

In past years, treatment for patients with hemophilia A has consisted of administration of cryoprecipitates (enriched in factor VIII) prepared from individual donors or lyophilized factor VIII concentrates prepared from plasma pools of up to 5000 donors. It is now possible to prepare factor Vlll by recombinant DNA technology. Such preparations are free of contaminating viruses (eg, hepatitis A, B, G, or HlV-1) found in human plasma but are at present expensive their use may increase if cost of production decreases. 

Inherited deficiencies of the clotting system that result in bleeding are found in humans. The most common is deficiency of factor VIII, causing hemophilia A, an X chromosome-hnked disease that has played a major role in the history of the royal families of Europe. Hemophilia B is due to a deficiency of factor IX its clinical Feamres are almost identical to those of hemophilia A, but the conditions can be separated on the basis of specific assays that distinguish between the two factors. 

PCCs contain the vitamin K-dependent factors II, VII, IX, and X. These agents represent another attempt to bypass the factor at which the antibody is directed (see Fig. 64-2). However, PCCs carry the risk of serious thrombotic complications. Porcine factor VIII is most useful when the inhibitor titer is less than 50 BU (see Fig. 64-2 for dose and frequency). Owing to its similarity to human factor VIII, porcine factor VIII participates in the coagulation cascade. However, most inhibitors have very weak neutralizing activity against it. Porcine factor VIII is a third-line agent (only after factor Vila and a PCC have failed) owing to a 15% incidence of cross-reactivity.15... 

Administration of porcine factor VIII, which may or may not cross-react with the antibodies raised against human factor Villa. (However, the immune system will soon begin to produce antibodies against the porcine clotting factor.)... 

Owing to the frequency of product administration, the purification procedure for recombinant factor VIII C must be particularly stringent. Unlike the situation pertaining when the product is purified from human blood, any contaminant present in the final product will be non-human and, hence, immunogenic. Sources of such contaminants would include ... 

Individuals who display a deficiency of factor IX develop haemophilia B, also known as Christmas disease. Although its clinical consequences are very similar to that of a deficiency of factor VIII, its general incidence in the population is far lower. Persons suffering from haemophilia B are treated by i.v. administration of a concentrate of factor IX. This was traditionally obtained by fractionation of human blood. Recombinant factor IX is now also produced in genetically engineered CHO cells (Table 12.2 and Box 12.1). 

Legaz, M.E., Schmer, G., Counts, R.B., and Davie, E.W. 1973. Isolation and characterization of human factor VIII (antihaemophilic factor). Journal of Biological Chemistry 248, 3946-3955. 

Wiliemer, H. Freeze-drying process data determination for human blood derivates with factor VIII as example. PDA Fourth International Congress, p. 142-151, Vienna, 1996. Copyright 1996, PDA Inc. Bethesda, Maryland, USA... 

NTP. 1994. Seventh annual report on carcinogens. Summary 1994. U.S. Department of Health and Human Services, iv-viii, 226-229. 

System 20. aquatic plants—bentos, plankton, coastal aquatic plants (XII) aquatic animals including bottom sediment invertebrates, fishes, amphibians, mammals, vertebrates, their biological reactions and endemic diseases (VIII) aerosols, atmospheric air (31, 32)—foodstuffs, forages (XV). Human poisoning through consumption of fish and other aquatic foodstuffs with excessive bioaccumulation of pollutants is the most typical example of biogeochemical migration and its consequences. 

A serious problem with some vegetables and particularly with fruit as a source of protein is the dilution of protein with carbohydrates so that an inordinate amount of calories must be consumed to obtain the needed amount of protein and amino acids for human growth. A survey of calories and protein content/100 g fresh weight of several different leaves, pods, seeds and tubers is shown in Table VIII. This indicates the desirability of certain leaves, pods and seeds over tubers, for example, as a protein source. 

To investigate these two questions, a parametric model of the Jacobian of human erythrocytes was constructed, based on the earlier explicit kinetic model of Schuster and Holzhiitter [119]. The model consists of 30 metabolites and 31 reactions, thus representing a metabolic network of reasonable complexity. Parameters and intervals were defined as described in Section VIII, with approximately 90 saturation parameters encoding the (unknown) dependencies on substrates and products and 10 additional saturation parameters encoding the (unknown) allosteric regulation. The metabolic state is described by the concentration and fluxes given in Ref. [119] for standard conditions and is consistent with thermodynamic constraints. 

Factor VIII from human plasma In-process control, semipreparative purification Anion Exchange disks and tubes [46]... 

Factor VIII from human plasma Purification, comparison with porous particle materials Affinity (peptides) disks [86]... 

Groupe de Recherche sur la Parole—Psychologie des Processus Cognitifs—University of Paris VIII and The Human Behavioral Laboratory—University of Iceland—Address (July) Masholar 6, 111 Reykjavik, Iceland tel. home (354)-557-43-55 Office (354)-569-45-85, e-mail bjalli mmedia.is... 

V, proximal anastomosis within the temporal component, and distal interconnection between the latter and the cervical component VI, two anastomotic rami sent from the buccal division of the cervical to the zygomatic part of the temporal VII, transverse ramus, from the trunk of the nerve, contributing to the buccal ramus formed by anastomosis between the two major divisions VIII, richly plexiform communications, especially within the temporal portion of the nerve. From Barry J. Anson, Atlas of Human Anatomy, p. 37. 

Y. Kurono, T. Furukawa, T. Tsuji, K. Ikeda, Esterase-Like Activity of Human Serum Albumin. VI. Reaction with p-Nitrophenyl Glycinate , Chem. Pharm. Bull. 1988, 36, 4068-4074 Y. Kurono, I. Kushida, H. Tanaka, K. Ikeda, Esterase-Like Activity of Human Serum Albumin. VIII. Reaction with Amino Acid p-Nitrophenyl Esters , Chem. Pharm. Bull. 1992, 40, 2169-2172. 

Y. Kurono, I. Kushida, H. Tanaka, K. Ikeda, Esterase-Like Activity of Human Serum Albumin. VIII. Reaction with Amino Acid p-Nitrophenyl Esters , Chem. Pharm. Bull. 1992, 40, 2169-2172. 

Besman M.J. and Shiba D. (1997), Evaluation of genetic stability of recombinant Human Factor VIII by peptide mapping and on-line mass spectrometric analysis. Pharm. Res. 14(8), 1092-1098. 

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