Bone marrow culture

L Vova TS. 1984. [Study of the mutagenic effect of 5 promising pesticides in mouse bone marrow cultured human peripheral blood lymphocytes, and in the yeast Saccharomyces-cerevisiae.] Tsitol Genet 18 455-457. (Russian)... 

Abe E, Yamamoto M, Taguchi Y, Lecka-Czernik B, O Brien CA, Economides AN, Stahl N, Jilka RL, Manolagas SC (2000) Essential requirement of BMPs-2/4 for both osteoblast and osteoclast formation in murine bone marrow cultures from adult mice antagonism by noggin. J Bone Miner Res 15 663-673... 

Devlin RD, Reddy SV, Savino R, Ciliberto G, Roodman GD (1998) IL-6 mediates the effects of IL-1 or TNF, but not PTHrP or l,25(OH)2D3, on osteoclast-like cell formation in normal human bone marrow cultures. J Bone Miner Res 13 393-399... 

Qu Q, Harkonen PL, Vaananen HK (1999) Comparative effects of estrogen and antiestrogens on differentiation of osteoblasts in mouse bone marrow culture. J Cell BioChem 73 500-507... 

Inaba, K. et al., Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor, J. Exp. Med., 176, 1693, 1992. 

Diagnosis Blood cultures require a prolonged period of incubation in the acute phase. Bone marrow cultures produce a higher yield. Confirmation requires phage-typing, oxidative metabolism, or genotyping procedure. Enzyme-Linked Immunosorbent Assays... 

Three years later, Lajtha, Oliver, and Ellis performed similar studies with human bone-marrow cultures exposed to 32P, or 14C-adenine. Control smears were treated with M HC1 at 60 °C for 6.5 min to remove 32P not incorporated into DNA. Grain counts were made over individual nuclei so that the rate of uptake into DNA could be estimated. The cycle time for the dividing cells in the culture was 40-48 h. DNA synthesis took 12-15 h in the second half of the cycle and was divided from mitosis by a 3-4 h non-synthesizing period (G2). 

Schoeters GER, Vander Plaetse F, Leppens H, et al. 1995. Haemopoietic and osteogenic toxicity testing in vitro using murine bone marrow cultures. Toxicol In Vitro 9 421-428. 

Nifontova IN, Ershler MA, Drize NI. Dynamics of precursor cell composition in bone marrow culture derived from mice deficient by tumor necrosis factor. BuU Exp Biol Med. 2003 135 285-288. 

In conclusion, we have shown that successful treatment of MI and I/R AKI with MSC holds substantial promise for the development of novel, MSC-based interventions that can improve the treatment of severe, and still largely therapy-resistant, clinical ischemic myocardial infarction as well as ARF that results from I/R injury. Pluripotent MSC, because of their versatility and the ease with which they can be harvested from the bone marrow, culture expanded, and engineered, appear to be a particularly well-suited stem cell type for these clinical indications. 

Mycobacterium Avium Complex (MAC) Blood, stool, sputum, bone marrow culture Microbiology laboratory BACTEC medium... 

Marx. M.P., Smith, S., Heyns, A.P van Tonder, I.Z. (1983) Fanconi s anemia a cytogenetic study on lymphocyte and bone marrow cultures utilizing l,2 3,4-diepoxybutane. Cancer Genet. Cytogenet., 9, 51-60... 

FIG. 15 Effect of lycopene on resorption of the calcium phosphate substrate coating of osteologic multitest slides in the presence of osteoclasts (Rao et al., 2003). (Lycopene I -Effect on osteoclasts Lycopene inhibits basal and parathyroid hormone-stimulated osteoclast formation and mineral resorption mediated by reactive oxygen species in ray bone marrow cultures. Reprint from Journal of Medicinal Food. 2003 6, pp. 69-78 by permission of Mary Ann Liebert, Inc., Publishers.)... 

Soma S, Matsumoto S,Takano-Yamamoto T. Enhancement by conditioned medium of stretched calvarial bone cells of the osteoclast-like cell formation induced by parathyroid hormone in mouse bone marrow cultures. Archs Oral Biol. 1996 42(3) 205-211. 

Schoeters et al. (1995) evaluated the hematopoietic and osteogenic toxicity of benzene, phenol, hydroquinone, and catechol in vitro using murine bone marrow cultures. Evaluation of toxicity to 3T3-fibroblasts was included to determine specific toxicity to marrow cells. Benzene and phenol showed little effect. However, hydroquinone inhibited proliferation of 3T3 cells and marrow precursor cells, and calcification of bone cells, although it was more specific for marrow cells. Catechol inhibited all cells, and showed no specificity. 

Abraham N. 1996. Hematopoietic effects of benzene inhalation assessed by long term bone marrow culture. Environ Health Perspect 104 (Suppl 6) 1277-1282. 

Hauser SP, Allewelt MC, Lipschitz DA. Effects of myelotoxic agents on cytokine production in murine long-term bone marrow cultures. Stem Cells 1998 16(4) 261-70. 

Deferoxamine has a strong depressant effect on proliferation of bone marrow cultures in vitro (72). On the other hand, deferoxamine improves hemopoiesis in patients with anemia, for example in rheumatoid arthritis, hemolysis, or myelodysplastic sjmdromes, and reduces transfusion dependency (73-78). The mechanism is unknown, but increased erythropoietin responsiveness secondary to iron chelation may play a role (77). 

Romeril KR, Duke DS, Hollings PE. Sulindac induced agranulocytosis and bone marrow culture. Lancet 1981 2(8245) 523. 

Clutterbuck, E.J., Hirst, E.M. and Sanderson, C.J. (1989). Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrow cultures comparison and interaction with IL-1, IL-3, IL-6 and GMCSF. Blood 73, 1504-1512. 

Fischer, H.G., Frosch, S., Reske, K. and Reske-Kunz, A.B. (1988). Granulocyte-macrophage colony-stimulating fector activates macrophages derived from bone marrow cultures to synthesis of MHC class II molecules and to augmented antigen presentation function. J. Immunol. 141, 3882-3888. 

An initial evaluation of myelotoxicity should be performed. If a compound is myelotoxic, there may be no need to proceed with additional evaluation. The methodology for bone marrow culture systems is published and well characterized. In vitro bone marrow culture systems are commercially available, and they would probably have to be modified slightly to accommodate in vitro exposure to test material. Assays of immunosuppression have been validated to predict the maximum tolerated dose (MTD) in humans. Their suitability for use in immunotoxicology should be determined and would require prevalidation. These assays are relatively expensive if human cells are used, and the standardized nature of commercial systems should provide good feasibility. 

Bone marrow culture - positive CSF culture - positive... 

Hinoshita F, Hardin JA, Sherr DH. 1992. Fluoranthene induces programmed cell death and alters growth of immature B cell populations in bone marrow cultures. Toxicology 73(2) 203-218. 

Resegotti L, Pistone MA, Testa D, et al. Bone marrow culture in patients treated with ticlopidine. Nouv Rev Fr Hematol 1985 27 19-22. 

Gotuzzo E, Carrillo C, Guerra J, Llosa L. An evaluation of diagnostic methods for brucellosis—The value of bone marrow culture. J Infect Dis. 1986 153(1) 122—125. 

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