Vector promoter

Expression of genes at a regularly low level, resulting in a low level of product, occurs in every organism. For recombinant proteins, overexpression which leads to considerably enhanced amounts of the recombinant protein is desired. Success of an attempt at recombinant overexpression cannot be predicted as of yet and depends on many factors, such as the protein to be expressed, host, vector, promoter, culture conditions, and, last but not least, the experience of the investigator. 

Phage display vector Promoter Secretion Antibody format used C-domains ir vector Sites heavy chali Sites light chain Tags gill Expression of soluble Ab Reference... 

McCarty, D. M., Monahan, P. E. and Samulski, R. J. (2001). Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis. Gene Ther. 8, 1248-1254. 

Cyanobacteria are procaryotic photosynthetic microorganisms and can provide a simple genetic transformation system. In this study, we established an efficient vector-promoter system for the introduction and expression of foreign... 

Hydroxyethylated cationic cholesterol derivatives in liposome vectors promote gene expression in the lung. Int J Pharm. 354 196-203 doi 10.1016/j.ijpharm.2007.10.051... 

Strain Vector Promoter Source of LDH pH control LA produced (g/L) Yield Reference... 

Registry (http partsregistry.org/Main Page), which can be used, supplemented, or extended by their projects. Available parts include cloning and expression vectors, promoters, reporters, sensors, regulators, and genetic circuits, among others. 

Vector Promoter Other features (size, marker) Source... 

If an antibody to the protein of interest is available, it is sometimes possible to use vector sequences, eg, the beta-galactosidase promoter sequence, to direct the transcription of the passenger DNA into messenger RNA and the translation of that mRNA into protein which can be recognized by the antibody. Although this method is somewhat less reHable than the use of nucleic acid probes, specialized vectors are available for this purpose. 

A variety of tiansformation techniques using E. co/ -ye st shuttle vectors and yeast selectable markets, as well as efficient yeast promoters and signal... 

Note that the strategies discussed thus far create hybrids in which the orientation of the DNA insert within the chimera is random. Sometimes it is desirable to insert the DNA in a particular orientation. For example, an experimenter might wish to insert a particular DNA (a gene) in a vector so that its gene product is synthesized. To do this, the DNA must be placed downstream from a promoter. A promoter is a nucleotide sequence lying upstream of a... 

A vector for in vitro expression of DNA inserts as RNA transcripts can be constructed by putting a highly efficient promoter adjacent to a versatile cloning site. Figure 13.15 depicts such an expression vector. Linearized recombinant vector DNA is transcribed in vitro using SPG RNA polymerase. Large amounts of RNA product can be obtained in this manner if radioactive ribonucleotides are used as substrates, labeled RNA molecules useful as probes are made. 

FIGURE 13.15 Expression vectors carrying the promoter recognized by the RNA polymerase of bacteriophage SPG are useful for making RNA transcripts in vitro. SPG RNA polymerase works efficiently in vitro and recognizes its specific promoter with high specificity. 

These vectors typically have a polylinker adjacent to the SPG promoter. Successive rounds of transcription initiated by SPG RNA polymerase at its promoter lead to the production of multiple RNA copies of any DNA inserted at the polylinker. Before transcription is initiated, the circular expression vector is linearized by a single cleavage at or near the end of the insert so that transcription terminates at a fixed point. 

FIGURE 13.17 A protein expression vector contains the hybrid promoter derived from fusion of the lac and trp promoters. Expression from pi A more than 10 times greater than expression from either the lac or trp promoter alone. Isopropyl-/3-D-thiogalacto-side, or IPTG, induces expression from pi as well as lac. 

The eukaryotic expression cassette is the part of an expression vector that enables production of a protein in a eukaryotic cell. The cassette consists of a eukaryotic promoter for mRNA transcription, the gene and an mRNA termination and processing signal (Poly-A signal). 

Gene Expression Analysis Gene Gun Gene Products Gene Promoter Gene Therapy Gene-therapy Vectors Gene Transfer... 

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