MRNA transcripts

Regulation of mRNA transcription and DNA bending by using triple helix forming oligonucleotide 99YGK194. 

The eukaryotic expression cassette is the part of an expression vector that enables production of a protein in a eukaryotic cell. The cassette consists of a eukaryotic promoter for mRNA transcription, the gene and an mRNA termination and processing signal (Poly-A signal). 

With respect to tissue distribution, the available data is limited to mRNA transcripts which, for all TAARs studied so far, are present at generally low levels when compared to e.g., 5-HT and DA receptors. The most... 

The entire population of mRNA transcripts in the cell, weighted by their expression levels. 

Ramagopal, S. (1987). Differential mRNA transcription during salinity stress in barley. Proceedings of the National Academy of Sciences, USA, 84, 94-8. 

Recently, the related phenomenon of RNA interference (RNAi) has attracted much attention [5]. RNAi occurs when a short (generally 21 nucleotides in length) double-stranded RNA (dsRNA) catalyticaUy represses the translation of a fully complementary mRNA sequence. The process appears to proceed via a complex formed between the antisense RNA strand and a protein with RNase activity [6]. Upon binding to the target mRNA sequence, the ribonucleoprotein complex initiates cleavage of the mRNA transcript thus preventing translation of intact protein. After dissociation from the truncated mRNAs, the ribonucleoprotein complex is free to act on other intact mRNAs. Such small interfering RNAs (siRNAs) have... 

Figure 37-1. This figure illustrates that genes can be transcribed off both strands of DNA. The arrowheads indicate the direction of transcription (polarity). Note that the template strand is always read in the 3 to 5 direction. The opposite strand is called the coding strand because it is identical (except for T for L) changes) to the mRNA transcript (the primary transcript in eukaryotic cells) that encodes the protein product of the gene.

Figure 38-5. Examples of the effects of deletions and insertions in a gene on the sequence of the mRNA transcript and of the polypeptide chain translated therefrom. The arrows indicate the sites of deletions or insertions, and the numbers in the ovals indicate the number of nucleotide residues deleted or inserted. Blue type indicates amino acids in correct order.

Jaffe, H.A., Buhl, R., Borok, Z., Trapnell, B. and Crystal, R.G. (1989). Activated alveolar macrophages express increased levels of cytochrome b245 heavy chain mRNA transcripts correlating with enhanced capacity to release oxidants. Clin. Res. 37, 477A. 

Intracellular replication of viral particles depends entirely upon successful intracellular transcription of viral genes with subsequent translation of the viral mRNA. Translation of viral or cellular mRNA is dependent upon ribosome formation. Normally, several constituent molecules interact with each other on the mRNA transcript, forming the smaller ribosomal subunit. Subsequent for-mation/attachment of the larger subunit facilitates protein synthesis. 

The product inhibits replication of human CMV (HCMV) via an antisense mechanism. Its nucleotide sequence is complementary to a sequence in mRNA transcripts of the major immediate early region (IE2 region) of HCMV. These mRNAs code for several essential viral proteins and blocking their synthesis effectively inhibits viral replication. 

Other solute transporters (solute carrier protein [SLC]) are also expressed in the alveolus. The mRNA transcripts of glucose transporters, GLUT1, GLUT4, GLUT5 and SGLT1, have been detected in freshly isolated rat ATII cells by... 

Perhaps one of the most exciting areas in neuroscience to which in situ hybridization has made significant contributions in recent years is the discovery that specific mRNA transcripts can be localized to certain compartments of neuronal cells (Steward and Schuman, 2003), such as axons (Tohda, 2003), dendrites (Ma and Morris, 2002 Bockers et al., 2004), cell bodies (Lu et al., 1998), and growth... 

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