Eukaryotic Expression Cassette

The eukaryotic expression cassette is the part of an expression vector that enables production of a protein in a eukaryotic cell. The cassette consists of a eukaryotic promoter for mRNA transcription, the gene and an mRNA termination and processing signal (Poly-A signal). 

The blasticidin deaminase gene is placed in a eukaryotic expression cassette to enable selection for the desired mammalian clones by using the nucleoside antibiotic blasticidin. 

Most DNA vaccines tested over the past decade have consisted of conventional plasmids with a eukaryotic expression cassette. The important elements of such plasmids are the promoter, the gene insert, the polyadenylation termination sequence, a bacterial origin of replication for production in Escherichia coli, and an antibiotic resistance gene for selection (see Fig. 1). Typically, strong viral promoters, such as the intermediate early promoter of cytomegalovirus with the intron A, are used. This ensures constitutive, high levels of antigen production in many tissue... 

ABC (ATP binding cassette) transporters are ubiquitous proteins that are expressed by prokaryotic and eukaryotic organisms. About 50 human ABC transporters are known, which are divided into seven different subfamilies, designated A-G. 

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