Triglycerides fractionation

Nova-Pak C18 column in a methanol water chloroform gradient.92 Choline chloride was added to the mobile phase. One review of techniques used in the analysis of triacylglycerols lists over 300 references on separations of the triglyceride fraction of fats using nonaqueous RPLC, aqueous RPLC, argen-tation chromatography, and other chromatographic methods.93... 

Practical use of the solidification and crystallization characteristics of milk lipids has been made in the manufacture of butter which is more easily spread than butter made conventionally. Based on the knowledge that temperature and mechanical manipulation can influence crystallization behavior, various methods of working butter have been devised to produce a softer product (Taylor et al. 1971 Schaap et al. 1981). Another approach has been to separate triglyceride fractions according to solidification or melting ranges and reblend fractions to achieve a softer butter (McGillivray 1972 Black 1975 Frede et al. 1980). 

Extraction of nonpolar compounds using equal volumes of sample and the Folsch mixture (2 1, chloroform/MeOH) gives a very broad polarity cut. Everything from steroids to triglycerides is pulled down into the bottom chloroform-rich layer. Extraction with methylene chloride from a sample acidified with sulfuric acid is more specific, pulling in steroids, fat-soluble vitamins, and free fatty acids. The triglyceride fraction can be extracted using i-PrOH/ hexane (1 9) with little emulsification. 

Fractionated triglyceride of coconut oil (medium-chain triglyceride) Fractionated triglyceride of palm seed oil (medium-chain triglyceride)... 

Although subsequent studies of Deuteromycotina (Table X) and Ascomycotina (Table XI), performed by Block [57] for the triglyceride fraction and by Cooney [58] for total fatty acids, confirmed the preponderance of unsaturated fatty acids (33 to 79%, but generally 58 to 79%) [58] and co3 and co6 acids, polyenic acids in C20 were only found in small quantities, and no acids were detected in C22.6 [58,59]. 

These recent studies show that the major fatty acids are palmitic (16 0), oleic (18 1 co9) and linoleic (18 2 (d6) acids, which most often account for 80 to 90% of total fatty acids [58] or of the triglyceride fraction [57]. These results are concordant with those of [60] in 11... 

A. Calculated Carbon Numbers and Number of Double Bonds of Triglyceride Fractions of LEAR (cv. Lobra) Oil... 

B. Main TG Types and Main Fatty Acid Contents of Triglyceride Fractions of LEAR Oil... 

The change in the 22 1 content with time on diet for the three species is shown in Fig. 2. The concentration of 22 1 in the cardiac triglycerides is compared to the concentration of this acid in the dietary oil and expressed as a ratio. As evident from these results, the 22 1 concentration of the rat heart triglycerides is the same as that of the dietary oil during the first week on the experimental diet. The ratio of 22 1 then decreases on continued feeding to a ratio of approximately 0.5 after 16 weeks. In the pig, the ratio was 0.2 throughout the 1 year study. The monkey shows a tendency to accumulate 22 1 in the cardiac triglyceride fraction. Erucic acid (22 1 n-9)... 

D. Experiments with Highly Purified Triglyceride Fractions Isolated... 

The results presented in Table XXI support the same conclusion that the triglycerides are in fact the cardiotoxic principle. In this experiment, a very highly purified triglyceride fraction was obtained by column chromatography (Fig. 6, Table XIX), and no reduction in lesion incidence or severity was found when diets containing this fraction (AC2) was fed to male Spra-gue-Dawley rats. A repetition of this experiment gave identical results (Experiment B in Table XXI). 

Myocardial Lesions in Male Sprague-Dawley Rats Fed Pure Triglyceride Fractions of Soybean Oil and LEAR (cv. Tower) Oil for 16 Weeks ... 

Oiticica oil triglycerides have been separated by silica column chromatography into four fractions a monoketo fraction (27.3%), dike to fraction (32.1%), a more polar triglyceride fraction (12.9%) and others (15.9%). The level of licanic acid was reported to be lower than that previously reported, i.e. 46% instead of 70-80% (Evans 1967). 

Separation of interesterified fats from various oils into their free fatty acid and mono-, di-, and triglyceride fractions was rapidly achieved on a silica column (ELSD) using a 90/10 iso-octane/IPA mobile phase [699], Baseline resolution was obtained along with complete elution in under 15 min. Tallow, sun oil, and sunflower oil elution profrIes are shown. 

One can demonstrate, for example, that the fractions of a naturally occurring lipid mixture, separated by TLC, are not contaminated by one another. This is shown in Fig. 94. A small amount of hot tripal-mitin was mixed with shark liver oil and separation then carried out with adsorption TLC. An autoradiograph of the chromatogram showed that the total radioactivity was in the triglyceride fraction. The glyceryl ether diesters were not contaminated with the triglycerides despite their very similar structure. 

Thin-layer and gas chromatography have been used to characterise the lipids in the protoplast membranes of some streptococci [44] and for analysis of the hydrocarbons from the micrococcus Sarcina lutea [2]. Diesters and alkenyl ether-esters of various diols have been found in the triglyceride fraction of a yeast, in the same way [12]. Using TLC, it... 

Two groups have reported that lipid extracts of mouse tumours contain compounds which riiigrate ahead of the triglyceride fractions on adsorbent layers [113, 191] these substances were considered at first to be methyl esters of fatty acids but more detailed studies of the material isolated through TLC, showed that they were alkyl diglycerides [191]. 

Study of Fatty Acids Making Up the Plasma Triglyceride Fraction in Normal and Thalassemic Subjects Using Gas Chromatography 1419,... 

Diet and Fatty Acid Distribution in Subcutanous Fats and in the Chol-sterol-Triglyceride Fraction of Serum of Young Infants J. Clin. Invest. 42 1-9 (1963) CA 58 12910g... 

The phospholipid fraction comprised 2.9% of the crude oil. The composition is 55.8% PC, 18.7% PE, 17.2% PI, 2.8% LpoPC and 5.5% others (Scheerens and Berry, 1986). The composition is very similar to soybean lecithins and may indicate possible commercial uses. The fatty acid profile of isolated phospholipids revealed interesting contrasts to common patterns of the triglycerides fraction. Greater proportions of myristic and palmitic acids (7.4% and 21.45%) were found in the phospholipid fraction. 

The triglycerid fraction was separated on AgNO impregnated silica gel layer and detected by sulfuric acid. The fatty acids of fractions was analysed by GLC. The apparatus CHROM41 column 10% polyethyleneglycoladipate on Chromosorb WHP, at 190 °C. 

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