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Tk assay

An in-vitro test with mammalian cells (chromosomal damage or mouse lymphoma tk assay)... [Pg.66]

Under Guideline S2B, the following standard test battery is recommended (1) a test for gene mutation in bacteria, (2) an in vitro test with cytogenetic evaluation of chromosomal damage with mammalian cells or an in vitro mouse lymphoma thymidine kinase (TK) assay, and (3) an in vivo test for chromosomal damage using rodent hematopoietic cells. [Pg.306]

The mouse lymphoma assay is in fact just one of several mammalian cell assays designed to determine increases in mutation rate. It focuses on the thymidine kinase (tk) assay in murine lymphoma cells (L5178Y), though tk data have also been produced from the human lymphoblastoid cell line TK6, and at the hgprt (hypox-anthine-guanine phosphoribosyl-transferase) locus in Chinese Hamster ovary or lung (V79) cells and mouse lymphoma cells. Since Ames mutation data and often... [Pg.254]

Amacher, D.E. Turner, GN. (1985) Tests for gene mutational activity in the L5178Y/TK assay system. Prog. Mutat. Res., 5, 487 96... [Pg.125]

Amacher, D.E. Paillet, S.C., and Turner, G.N. (1979b). Utility of the mouse lymphoma L5178Y/TK assay for the detection of chemical mutagens, in Banbury Report 2, Mammalian Cell Mutagenesis The Maturation of Tkst Systems, HsiE, A.W., O Neill, J.R, and McElheny, V.K, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York). [Pg.131]

Cameron, T.P., Rogers-Back, A.M., Lawlor, T.E., Harbell, J.W., Seifried, H.E. Dunkel, V.C. (1991) Genotoxicity of multifunctional acrylates in the /zwo e//a/nianimahan-niicrosome assay and mouse lymphoma TK+ - assay. Environ, mol. Mutag., 17,264-271... [Pg.1229]

Amacher DE, Turner GN. 1982. Mutagenic evaluation of carcinogens and noncarcinogens in the L5178Y/TK assay utilizing postmitochondrial fractions (S9) from normal rat liver. Mutat Res 97 49-65. [Pg.90]

An in vitro cytogenetic test in mammahan cehs or in vitro mouse lymphoma tk + assay. [Pg.160]

An in-vitro test with cytogenetic evaluation of chromosomal damage with mammalian cells or an in-vitro mouse lymphoma thymidine kinase (tK) assay... [Pg.46]

D-Limonene was not mutagenic in four strains of Salmonella, did not significantly increase the number of trifluorothymidine-resistant cells in the mouse L5178Y/TK" assay, and did not induce chromosomal aberrations or sister chromatid exchanges in cultured CHO cells. All assays were conducted in the presence and absence of exogenous metabolic activation. [Pg.1534]

Blackbum GR, Dooley JF, Schreiner CA. 1991. Specific identification of formaldehyde mediated mutagenicity using the mouse lymphoma L5178Y TK +/- assay supplemented with formaldehyde dehydrogenase. In Vitro Toxicol 4 121-132. [Pg.371]

Figure 10.1. Correlation data for the sensitivity and specificity for single in vitro genotoxicity assays and assay combinations. Ames, Salmonella reverse mutation test introduced by B. Ames MLA, tk assay using the L5178Y mouse lymphoma cell line Cab, chromosome aberration test with different mammalian cell types MN, in vitro micronucleus assay with different mammalian cell types. [Data according to Kirkland et al. (2005a).]... Figure 10.1. Correlation data for the sensitivity and specificity for single in vitro genotoxicity assays and assay combinations. Ames, Salmonella reverse mutation test introduced by B. Ames MLA, tk assay using the L5178Y mouse lymphoma cell line Cab, chromosome aberration test with different mammalian cell types MN, in vitro micronucleus assay with different mammalian cell types. [Data according to Kirkland et al. (2005a).]...
II. The in vitro micronucleus test is endorsed as an alternative option to the in vitro chromosome aberration test and the mouse l)miphoma tk assay. [Pg.245]

This section also covers issues for PK/TK assays that are discussed later.)... [Pg.177]

Bacteriai reverse mutation assay In vitro mouse iymphoma tk assay Chromosomai aberration in mammaiian ceiis Carcinogenicity... [Pg.368]

In this context, new assays for TK activity detection have been recently developed. This chapter describes the recent in vivo and in vitro TK assays based on cascade reactions involving one or more auxiliary enzymes and/or protein and/or nonprotein agents (Scheme 15.3). [Pg.316]

Scheme 15.3 TK assays based on cascade reactions. ADH, alcohol dehydrogenase TPI, triose phosphate isomerase BSA, bovine serum albumin PPO, polyphenoloxidase. Scheme 15.3 TK assays based on cascade reactions. ADH, alcohol dehydrogenase TPI, triose phosphate isomerase BSA, bovine serum albumin PPO, polyphenoloxidase.
Scheme 15.4 TK assay based on NADH-dependent dehydrogenase as auxiliary enzyme. Scheme 15.4 TK assay based on NADH-dependent dehydrogenase as auxiliary enzyme.
See other pages where Tk assay is mentioned: [Pg.209]    [Pg.254]    [Pg.114]    [Pg.304]    [Pg.312]    [Pg.765]    [Pg.39]    [Pg.31]    [Pg.160]    [Pg.587]    [Pg.245]    [Pg.247]    [Pg.250]    [Pg.277]    [Pg.331]    [Pg.333]    [Pg.854]    [Pg.38]    [Pg.186]    [Pg.187]    [Pg.138]    [Pg.158]    [Pg.319]   
See also in sourсe #XX -- [ Pg.329 , Pg.331 , Pg.333 ]



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