Tissue-engineered application

Multiple applications for resilin-like polypeptides have garnered renewed research interest since the report of the first recombinant resilin in 2005. The excellent mechanical properties of the resilin-like polypeptides has directed investigation toward their use as high-performance materials and in tissue engineering applications. It is widely acknowledged that cells interact and take cues from their microenvironment and, therefore, the development of polymeric scaffolds to mimic the extracellular matrix and drive desired cell or tissue responses has been of wide interest. To this end, our laboratories have developed a modular resilin-like polypeptide (RLP12) (see Fig. 20) that contains not only twelve repeats of the... 

Pradhan S, Farach-Carson MC (2010) Mining the extracellular matrix for tissue engineering applications. Regen Med 5 961-970... 

H. Adeli, S. H. S. Zein, S. H. Tan, H. M. Akil, A. L. Ahmad, Synthesis, characterization and biodegradation of novel poly(L-lactide)/multiwalled carbon nanotube porous scaffolds for tissue engineering applications., Current Nanoscience, vol. 7, pp. 323-333,2011. 

Bioreactor Developments for Tissue Engineering Applications by the Example of the Bioartificial Liver... 

Silva et al. (2006) studied starch-based microparticles as a novel strategy for tissue engineering applications. They developed starch-based microparticles, and evaluated them for bioactivity, cytotoxicity, ability to serve as substrates for cell adhesion, as well as their potential to be used as delivery systems either for anti-inflammatory agents or growth factors. Two starch-based materials were used for the development of starch-based particulate systems (1) a blend of starch and polylactic acid (SPLA) (50 50 w/w) and (2) a chemically modifled potato starch, Paselli II (Pa). Both materials enabled the synthesis of particulate systems, both polymer and composite (with BG 45S5). A simple solvent extraction method was employed for the synthesis of SPLA and SPLA/BG microparticles, while for Pa and Pa/BG... 

Silva, G. A., Coutinho, O. R, Ducheyne, R, Shapiro, I. M., Reis, R. L. (2006). Starch-based microparticles as a novel strategy for tissue engineering applications. Key Engg. Mater., 309-311, 907-910. 

These dendrimers expand the repertoire of polymers available for study. Current investigations are primarily limited to linear polymers that possess ill-defined solution structures and fewer hydroxyl groups for further modification. The introduction of biocompatible building blocks (e.g., glycerol and lactic acid) augments the favorable and already known physical properties of dendrimers. These properties are likely to facilitate the design of new materials for specific biomedical and tissue engineering applications. 

Girotti et al. (2004) have designed and bio-produced elastin-like protein polymers (ELP) which contain biofunctional motifs with cell adhesion sequences required for tissue-engineering applications. The protein polymer contained periodically spaced fibronectin CS5 domains enclosing the cell attachment sequence REDV. The overall sequence,... 

V. E. Santo, A. M. Frias, M. Carida, R. Cancedda, M. E. Gomes, J. F. Mano, and R. L. Reis, Carrageenan-based hydrogels for the controlled delivery of PDGF-BB in bone tissue engineering applications, Biomacromolecules, 10 (2009) 1392-1401. 

Crosslinkable bioresorbable hydrogel block copolymer compositions, (V), were prepared by Loomis [6] for implantable prostheses and as scaffolding for tissue engineering applications. 

Heteroatom biodegradable and electrically conducting polymers, (IV), effective for tissue engineering applications were prepared by Schmidt [4] and used in spinal cord regeneration, wound healing, and bone repair. 

In the future, nanotubes and nanofibers can be administered systemically, if the problem of their toxicity is addressed, for example, by appropriate polymer coating. In this respect, the continuous nanofibers are more likely to be used in implants or tissue engineering applications. 

Nguyen KT and West JL. Photopolymerizable hydrogels for tissue engineering applications. Biomaterials 2002 23 4307-4314. 

Using a similar procedure, a composite material for tissue engineering applications composed of HA and carboxymethylchitosan was obtained by a coprecipitation method. In vitro tests exhibited a great potential of this class of materials for bone tissue-engineering applications.79... 

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