Elastin-like protein polymers

Girotti et al. (2004) have designed and bio-produced elastin-like protein polymers (ELP) which contain biofunctional motifs with cell adhesion sequences required for tissue-engineering applications. The protein polymer contained periodically spaced fibronectin CS5 domains enclosing the cell attachment sequence REDV. The overall sequence,... 

Dandu, R., Von Cresce, A., Briber, R. et al. (2009) Silk-elastin-like protein polymer hydrogels Influence of monomer sequence on physicochemical properties. Polymer, 50, 366-374. 

Hatefi, A., Cappello, J. and Ghandehari, H. (2007) Adenoviral gene delivery to solid tumors by recombinant silk-elastin-like protein polymers. Pharmaceutical Research, 24,773-779. 

Cresce, A. V., Dandu, R., Burger, A. et al. (2008) Characterization and real-time imaging of gene expression of adenovirus embedded silk-elastin-like protein polymer hydrogels. Molecular Pharmaceutics, 5, 891-897. 

Ehnerman, A.A., CappeUo, J., Ghandehari, H. and Hoag, S.W. (2002) Solute diffusion in genetically engineered silk-elastin-like protein polymer hydrogels. Journal of Controlled Release, 82,277-287. 

Qiu, W.G., Teng, W.B., CappeUo, J. and Wu, X. (2009) Wet-spinning of recombinant SUk-elastin-like protein polymer fibers with high tensile strength tmd high deformability. Biomacromolecules, 10, 602-608. 

Anumolu, R., Gustafson, J.A., Magda, J.J. et al. (2011) Fabrication of highly uniform nanoparticles from recombinant sUk-elastin-like protein polymers for therapeutic agent delivery. ACS Nano, 5, 5374-5382. 

Price, R., Gustafson, J., Greish, K. et al. (2012) Comparison of silk-elastin-like protein polymer hydrogel and poloxamer in matrix-mediated gene deUvery. International Journal of Pharmaceutics, 427, 97-104. 

Xia, X., Xu, Q., Hu, X. et al. (2011) Tunable self-assembly of genetically engineered sdk-elastin-like protein polymers. Biomacromolecules, 12,3844—3850. 

Reactive noncanonical amino adds have also been integrated into elastin-like protein polymers to enhance their application as biomaterials. The Tinell laboratory has used the photoreactive amino add p-azidophenylalanine to pattern elastin-like protein polymers on surfaces. " Zhang et d. used p-azidophenylalanine to photochemically cross-link an elastin-leudne zipper protein to a glass surface. A target protein linked to a second leudne zipper domain can then be captured from solution and patterned on the surface by dimerization of the leucine zipper domains. Carrico et al. used similar p-azidophenylalanine-fiinctionalized elastin protein polymers... 

Protein engineering strategies have also allowed the incorporation of biological function into elastin-like protein polymers and SELPs. The incorporation of cell-binding amino acid seqnences, such as RGD (82,142) and REDV (from the CS5 domain of fibronectin) (66) (Table 2), permits endothelial cell adhesion to... 

Girotti A, Reguera J, Rodriguez-Cabello JC, Arias FJ, Alonso M, Testera AM (2004) Design and bioproduction of a recombinant multi(bio)functional elastin-like protein polymer containing cell adhesion sequences for tissue engineering purposes. J Mater Sci Mater Med 15 479 84... 

A particular kind of materials are the silk-elastin-like protein polymers (SELPs), consisting of the repeating units of silk and elastin blocks, which combine a set of outstanding physical and biological properties of silk and elastin. SELPs can undergo tunable... 

Ravi S, Krishnamurthy VR, Caves JM, Haller CA, Chaikof EL [2012], Maleimide-thiol coupling of a bioactive peptide to an elastin-like protein polymer. Acta Biomater, 8,627-635. 

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