Solid supports microscope slides

Nonetheless, both these adsorbants are powdered and require a solid support. Microscope slides are extremely convenient. To keep the powder from just falling off the slides, manufacturers add a gypsum binder (plaster). Adsorbants with the binder usually have a G stuck on the name or say For thin-layer use on the container. 

Classic solid phase substrates used in biotesting, such as microtiter plates, membrane filters or microscope slides, have been the first supports used for NA immobilization in array fabrication [27]. Desired attributes of any DNA array substrate include (i) chemical homogeneity (ii) thermal and chemical stability (iii) ability to control surface chemical properties such as polarity or hydrophobicity (iv) ability to be activated with a wide range of chemical functionalities (v) reproducibihty of the surface modification processes involved (vi) inert with respect to enzymatic activity especially ones involved in DNA manipulation and (vii) ultra-low intrinsic fluorescence. 

In this approach, NAs are directly deposited onto a glass support using a robot able to deliver with high precision a sample to a specific x y programmed location. The NA sample is loaded into a spotting pin (highly miniaturized stainless-steel fountain-pen nibs with a gap) by capillary action, and small volumes are transferred to a solid surface, such as a microscope slide, by direct physical contact between the pin and the solid substrate. Spot size depends on the acceleration of the pen towards and away from the slide, and the surface tension of the slide. After the first spotting cycle, the pin is washed and a second sample is then transferred to an adjacent address. A robotic control system and multiplexed print heads allow the automated immobilization of many different probes simultaneously onto the slide [29]. 

Cells or tissue sections under study are immobilized onto a solid support to facilitate later manipulation. The biological materials are generally secured to a glass slide on which they subsequently can be viewed with a microscope. This can be accomplished by growing adherent cells on a slide or chemically attaching the materials to the slide. 

Peptide microarrays are prepared by immobilizing many peptide molecules on the surface of a solid support in a small area in an addressable fashion. The immobilization can be achieved via in situ synthesis or chemical ligation through a covalent bond. A hydrophihc linker between the sohd surface and the peptide usually is added to minimize steric hinderance caused by the sohd support. The most commonly used solid support for microarray printing is a standard microscope glass slide. Other solid supports also have been used such as polystyrene, nitrocellulose membranes, PVDF membranes, Hybond ECL membranes, gold surfaces, and chemical vapor deposited diamond films. 

In situ hybridization is a specialized type of solid-support assay in which morphologically intact tissue, cells, or chromosomes affixed to a glass microscope slide provide the... 

Microscope glass slides (Perfecta, Brazil) were used as the solid support for the drying of the films of pasty material, having the following dimensions 48.5 mm long, 24.5 mm wide, and 1.0 mm thick. 

Like paper chromatography, thin-layer chromatography is a form of plane chromatography in that the stationary phase is held on a plane rather than in a column. Table 12.1 lists important stationary phases used in TLC along with the respective predominant sorption process operative with each of them. The solid phase is supported on to glass, metal or a plastic substance. (Microscope slides... 

Miniaturized, multiplexed, solid-phase immunoassays were developed in the 1980s using protein microdots spotted manually onto nitrocellulose sheets and other solid supports. It was shown quite clearly that this multianalyte immunoassay format was far more sensitive than standard immunoassays carried out in microtiter dishes because the sample volumes were so much smaller. Over the last few years, these devices have evolved into protein biochips, in which microdots of antibodies or protein analytes are arranged in a dense miniature grid on a solid support such as a microscope slide. Two types of... 

The method consists of placing a sample of the powder in the beam of a conventional X-ray diffraction machine and measuring the width of a characteristic line for the solid. The powder is usually prepared by mixing with an organic adhesive, a few drops of the slurry being placed on a glass microscope slide and allowed to dry. The slide then serves to support the powder in the instrument. It is not necessary to disperse the powder (as is done in sample... 

Figure 2 Measured scattering spectra of a single gold nanoparticle (left, dashed line) and a single silver nanoparticle (right, solid line). Both nanoparticles are supported by a glass slide. Measurements are taken on air in a evanescent field microscope.

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