Protein biochips

Bodovitz S, Joos T, Bachmann J. Protein biochips the calm before the storm. Drug Discovery Today 2005 10 283-7, 2005. 

Wright, G. L., Cazares, L. H., Leung, S.-M., Nasim, S., Adam, B.-L., Yip, T.-T., Schellhammer, P. F., Gong, L., and Vlahou, A. (2000). ProteinChip surface enhanced laser desorption/ionization (SELDI) mass spectrometry a novel protein biochip technology for detection of prostate cancer biomarkers in complex protein mixtures. Prostate Cancer and Prostatic Diseases 2, 264-276. 

Emili AQ et al. Large-scale functional analysis using peptide or protein arrays. Nature Biotechnol 2000 18 393-397. Fung ET et al. Protein biochips for differential profiling. Curr Opin Biotechnol 2001 12 65-69. 

No matter what kind of biochip is chosen for use, the first and sometimes the most important step to construct a biochip is to effectively immobilize proteins or biomolecules on a solid surface. The ideal properties of protein biochip surface are ... 

Huels and coworkers (2002) idenfified several areas within the drug development process where protein biochips have potential applicahon. The first... 

Huels, C., Muellner, S., Meyer, H.E., and Cahill, D.J., The impact of protein biochips and microarrays on the drug development process. Drug Discovery Today, 7, S119-S124, 2002. 

Fung, E.T., Thulasiraman, V., Weinberger, S.R., and Dalmasso, E.A., Protein biochips for differential profiling, Curr. Opinion Biotechnol., 12, 65-69, 2001. Garland, P.B. and Serafinowski, P.J., Effects of stray light on the fidelity of photo-directed oligonucleotide array synthesism Nucleic Acid Res., 30(19), 1-9, 2002. 

Huang, T. T., Sturgis, J., Gomez, R., Geng, T., Bashir, R., Bhunia, A. K., Robinson, J. P., and Ladisch, M. R. (2003a). Composite surface for blocking bacterial adsorption on protein biochips. Biotechnol. Bioeng. 81, 618-624. 

Wong, Y.F., Cheung, T.H., Lo, K.W., Wang, V.W., Chan, C.S., Ng, T.B., Chung, T.K., and Mok, S. C. 2004. Protein profiling of cervical cancer by protein-biochips Proteomic scoring to discriminate cervical cancer from normal cervix. Cancer Lett. 211, 227-234. 

Wong YF, Cheung TH, Lo KW, et al. Protein profiling of cervical cancer by protein-biochips Proteomic scoring to discriminate cervical cancer from normal cervix. Cance Lett 2004 211(2) 227-234. 

Rosty C, Christa L, Kuzdzal S, Baldwin WM, Zahurak ML, Carnot F, Chan DW, Canto M, Lillemoe KD, Cameron JL, Yeo CJ, Hruban RH, Goggins M. Identification of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology. Cancer Res 2002 62 1868-1875. 

Maddalena, A.S., Papassotiropoulos, A., Gonzalez-Agosti, C et al. (2004) Cerebrospinal fluid profile of amyloid beta peptides in patients with Alzheimer s disease determined by protein biochip technology. Neurodegener. Dis. 1, 231-235. 

Rusmini F, Zhong Z, Feijen J (2007) Protein immobilization strategies for protein biochips. Biomacromol 8 1775-1789... 

Protein Biochips in the Proteomic Field Table 7.1 Surface. 

From these results, a smaller protein subset containing approximately 50-200 different, purified proteins will be generated and subjected to further qualitative and quantitative on protein biochips. Following bioinformatical analysis, protein antigens will be determined which may be associated with the respective autoimmune disease following confirmation by a second method such as western immunoblotting. 

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