Glass microscope slide

The most common method to measure the compatibility of resins with other substances is to dissolve both materials in a mutually compatible solvent, and to cast a film on a glass slide. After solvent evaporation, a compatible system gives a clear film, while incompatibility results in an opaque film. A more accurate procedure is to melt the resin and the substance under a phase microscope, and compatibility is observed on the film after cooling. 

An interesting approach involved microscopic observation of fretting corrosion a glass slide mounted on the stage of a microscope was used for the bearing surface which pressed against a spherical specimen being vibrated by a solenoid... 

Figure 7.3 shows the two-beam photon-force measurement system using a coaxial illumination photon force measurement system. Two microparticles dispersed in a liquid are optically trapped by two focused near-infrared beams ( 1 pm spot size) of a CW Nd YAG laser under an optical microscope (1064 nm, 1.2 MWcm , lOOX oil-immersion objective, NA = 1.4). The particles are positioned sufficiently far from the surface of a glass slide in order to neglect the interaction between the particles and the substrate. Green and red beams from a green LD laser (532 nm, 21 kWcm ) and a He-Ne laser (632.8 nm, 21 kW cm ) are introduced coaxially into the microscope and slightly focused onto each microparticle as an illumination light (the irradiated area was about 3 pm in diameter). The sizes of the illumination areas for the green and red beams are almost the same as the diameter of the microparticles (see Figure 7.4). The back scattered light from the surface of each microparticle is...

Phase dilution test Place two emulsion droplets on a glass slide and add a droplet of one component to each emulsion droplet, stir, and observe under a microscope. This test is based on the principle that an emulsion can only be diluted with the liquid that constitutes the continuous phase. 

A reasonable objection to any in vitro model is whether it accurately mirrors the actual process. A strength of this model is that the peptides in the array, mounted on the microscope glass slide, are the very same as the antibody epitopes in the native proteins. Therefore, the types of formaldehyde-induced chemical reactions at or near the epitope are the same as would likely occur in a tissue sample. An additional strength of the model is that the experimental data using the peptide array completely account for the loss of immunoreactivity after formalin fixation and the recovery of immunoreactivity after antigen retrieval (Fig. 16.5). Nonetheless, our data do not prove that the model accurately represents formaldehyde reactions in tissue specimens. For example, our data do not exclude other causes of steric interference. 

A thin, uniform layer of cells, stained by Papanicolaou and Diff-Quik methods is prepared on glass slides for microscopic examination. 

In all microscopic methods, sample preparation is key. Powder particles are normally dispersed in a mounting medium on a glass slide. Allen [7] has recommended that the particles not be mixed using glass rods or metal spatulas, as this may lead to fracturing a small camel-hair brush is preferable. A variety of mounting fluids with different viscosities and refractive indices are available a more viscous fluid may be preferred to minimize Brownian motion of the particles. Care must be taken, however, that the refractive indices of sample and fluid do not coincide, as this will make the particles invisible. Selection of the appropriate mounting medium will also depend on the solubility of the analyte [9]. After the sample is well dispersed in the fluid, a cover slip is placed on top... 

Other TIRF configurations for inverted microscopes have been employed. Figure 7.10 shows an alternative system.(42) Instead of a prism fixed with respect to the beam as above, the prism is fixed with respect to the sample. The glass slide substrate propagates the incident beam toward the... 

A dilute emulsion comprising oil drops dispersed in an aqueous solution was sheared between two glass slides under an optical microscope [149]. Original drops were deformed into long threads (Fig. 1.13a) that broke in numerous identical aligned and regularly spaced droplets (Fig. 1.13b). One can distinguish some very... 

A recent report describes the radioactive labeled chromium (iii) ion adsorption on stearic acid LB films. The adsorption of chromium (iii) on a stearic acid monolayer on the surface of CrCl3 was described. Stearic acid monomolecular films on I0 3 M CaCl2 subsolutions were deposited in paraffin-coated microscope glass slides by the LB technique (pH range 2-9). 

Finally, microscopic examination of samples often requires their preparation as cross sections or thin sections, or by mounting the sample on a glass slide by means of a mounting medium. For preparing thin and cross sections, samples are embedded in a polymer solution. After curing of the polymer, the thin or cross section is obtained by polishing the embedded sample with SiC abrasive disks. Aluminum suspensions or diamond paste are occasionally employed in a final polishing step. 

B) Form. For Type I LA spread a thin layer of crysts on a glass slide, allow to dry in the air at RT and examine under a microscope using a magnification 150 (approx). If... 

Check the extent of cell wall breakage in the pellet by placing drops of the homogenate on glass slides and then adding a drop of Ponceau 2R (see Basic Protocol 2, step 7) and iodine in potassium iodide solution (see Basic Protocol 1, step 4), respectively. View with a compound light microscope. 

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